Cell Tracking

Grimm, Jan; Kircher, Moritz F.; Weissleder, Ralph

doi:10.1007/s00117-006-1449-5

Cited by 25 publications

(12 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Direct labeling involves a relatively simple step of in vitro incorporation of the marker molecule before the cell therapy. 20 A range of molecules can be used, and this technology is considered fairly well established and yields consistent and reproducible results. SPIONs, fluorescent dyes, or radionuclides can be used as probes to directly prelabel stem cells for noninvasive tracking.…”

Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

View full text Add to dashboard Cite

Superparamagnetic iron oxide nanoparticles (SPIONs) have been used for diagnoses in biomedical applications, due to their unique properties and their apparent safety for humans. In general, SPIONs do not seem to produce cell damage, although their long-term in vivo effects continue to be investigated. The possibility of efficiently labeling cells with these magnetic nanoparticles has stimulated their use to noninvasively track cells by magnetic resonance imaging after transplantation. SPIONs are attracting increasing attention and are one of the preferred methods for cell labeling and tracking in preclinical and clinical studies. For clinical protocol approval of magnetic-labeled cell tracking, it is essential to expand our knowledge of the time course of SPIONs after cell incorporation and transplantation. This review focuses on the recent advances in tracking SPION-labeled stem cells, analyzing the possibilities and limitations of their use, not only focusing on myocardial infarction but also discussing other models.

show abstract

Section: Labeling Stem Cells and Molecular Imaging Methodsmentioning

confidence: 99%

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Jasmin¹,

Souza²,

Louzada³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…PET suffers from other limitations, such as the high uptake of 18 F-fluorodeoxyglucose ( 18 F-FDG) in normal liver tissue, its limited spatial resolution, and the drawback of exposure to ionizing radiation. 4–6 Ultrasound is the modality that is currently used most frequently for metastatic survey of the liver in the operating room. 7–8 However, ultrasound has limited sensitivity and signal specificity, resulting in the difficulty of distinguishing cancer from normal tissue and inability to visualize microscopic disease.…”

mentioning

confidence: 99%

Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles

et al. 2016

Self Cite

View full text Add to dashboard Cite

Complete surgical resection is the first-line treatment for most liver malignancies. This goal would be facilitated by an intraoperative imaging method that enables more precise visualization of tumor margins, and detection of otherwise invisible microscopic lesions. To this end, we synthesized silica-encapsulated surface-enhanced Raman scattering (SERS) nanoparticles (NPs) that act as a molecular imaging agent for liver malignancies. We hypothesized that, after intravenous administration, SERS NPs would avidly home to healthy liver tissue, but not to intrahepatic malignancies. We tested these SERS NPs in genetically engineered mouse models of hepatocellular carcinoma and histiocytic sarcoma. After intravenous injection, liver tumors in both models were readily identifiable with Raman imaging. In addition, Raman imaging using SERS NPs enabled detection of microscopic lesions in liver and spleen. We compared the performance of SERS NPs to fluorescence imaging using Indocyanine Green (ICG). We found that SERS NPs delineate tumors more accurately and are less susceptible to photobleaching. Given the known advantages of SERS imaging, namely high sensitivity and specific spectroscopic detection, these findings hold promise for improved resection of liver cancer.

show abstract

“…Superparamagnetic iron oxide nanoparticles have found widespread applications in the biomedical field spanning in vitro diagnostic tests such as nanosensors, 1−4 in vivo imaging 5−9 and therapies such as magnetic fluid hyperthermia 10,11 or drug delivery. 12,13 Recent investigations have also explored the capability of controlling the position or temperature of magnetic nanoparticles within cells and tissues by remote application of magnetic fields.…”

mentioning

confidence: 99%

Dynamic Magnetic Fields Remote-Control ApoptosisviaNanoparticle Rotation

Zhang

Kircher

Koch³

et al. 2014

ACS Nano

Self Cite

193

197

View full text Add to dashboard Cite

The ability to control the movement of nanoparticles remotely and with high precision would have far-reaching implications in many areas of nanotechnology. We have designed a unique dynamic magnetic field (DMF) generator that can induce rotational movements of superparamagnetic iron oxide nanoparticles (SPIONs). We examined whether the rotational nanoparticle movement could be used for remote induction of cell death by injuring lysosomal membrane structures. We further hypothesized that the shear forces created by the generation of oscillatory torques (incomplete rotation) of SPIONs bound to lysosomal membranes would cause membrane permeabilization, lead to extravasation of lysosomal contents into the cytoplasm, and induce apoptosis. To this end, we covalently conjugated SPIONs with antibodies targeting the lysosomal protein marker LAMP1 (LAMP1-SPION). Remote activation of slow rotation of LAMP1-SPIONs significantly improved the efficacy of cellular internalization of the nanoparticles. LAMP1-SPIONs then preferentially accumulated along the membrane in lysosomes in both rat insulinoma tumor cells and human pancreatic beta cells due to binding of LAMP1-SPIONs to endogenous LAMP1. Further activation of torques by the LAMP1-SPIONs bound to lysosomes resulted in rapid decrease in size and number of lysosomes, attributable to tearing of the lysosomal membrane by the shear force of the rotationally activated LAMP1-SPIONs. This remote activation resulted in an increased expression of early and late apoptotic markers and impaired cell growth. Our findings suggest that DMF treatment of lysosome-targeted nanoparticles offers a noninvasive tool to induce apoptosis remotely and could serve as an important platform technology for a wide range of biomedical applications.

show abstract

Cell Tracking

Cited by 25 publications

References 76 publications

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Tracking stem cells with superparamagnetic iron oxide nanoparticles: perspectives and considerations

Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles

Dynamic Magnetic Fields Remote-Control ApoptosisviaNanoparticle Rotation

Contact Info

Product

Resources

About