Cell, Tissue, and Organoid Cultures of Blood Vessels

Martin, George M.; Ogburn, Charles E.

doi:10.1016/b978-0-12-598303-7.50007-2

Cited by 6 publications

(5 citation statements)

References 138 publications

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“…For fetal bovine aortic endothelial cells, MEMU' (which, unlike MEM, contains all of the nucleosides found in nucleic acids) was judged to be superior to all other tested media, including MEM and M199 which are most commonly employed in culturing vascular endothelial cells (Gimbrone, 1976;Martin and Ogburn, 1977). In accord with the findings of Duthu and Smith (1980), we observed that MEM was better than M199 for supportingcell growth in cultures plated at initially moderate cell densities.…”

Section: Discussionsupporting

confidence: 88%

Nucleoside requirements for the in vitro growth of bovine aortic endothelial cells

Fenselau

Kaiser

Wallis

1981

Journal Cellular Physiology

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The nutritional needs of cultured fetal bovine aortic endothelial cells were studied with regard to their nucleotide metabolism. When Medium 199 containing calf serum was supplemented with up to 5 microgram/ml of the deoxyribo- or ribonucleosides found in DNA or RNA, the rate of endothelial cell growth increased. The effect was entirely attributable to the pyrimidine nucleosides. The combination of deoxythymidine and deoxycytidine was much more effective than either deoxyribonucleoside used alone or than the combination of uridine and cytidine. Addition of deoxythymidine and deoxycytidine (each at 1 microgram/ml) to the medium supported the growth of endothelial cell cultures from initially sparse populations (ca. 50 cells/cm2), even at low concentrations (1%) of fetal bovine serum. The pyrimidine deoxyribonucleosides on their own were unable to stimulate cell growth; other bonafide growth stimulatory factors, such as those present in serum, serum dialysates, or retinal extracts, were needed in the medium to signal the initiation of DNA synthesis and cell replication. The significance of these findings with respect to improving cell performance under in vitro conditions and controlling endothelial cell growth in vivo are discussed.

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Section: Discussionsupporting

confidence: 88%

Nucleoside requirements for the in vitro growth of bovine aortic endothelial cells

Fenselau

Kaiser

Wallis

1981

Journal Cellular Physiology

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“…Also, intraspecific genetic differences may play a role as suggested from the fact that fibroblast cultures from humans who age prematurely, Werner syndrome and progeria, may undergo fewer PDs if compared to normal cells (8,35,36). However, it should be noted that the timing of the growth crisis and chromosomal changes obtained here are within the range ofthe variation previously reported for the well-studied species such as man (4,6), mouse (18)(19)(20), rat (13,14), and rabbit (21).…”

Section: Resultsmentioning

confidence: 44%

“…In animal cell cultures such as mouse, rat, and rabbit, phase III has been demonstrated prior to or coincidentally with the establishment of many continuous cell lines (13,14,(18)(19)(20)(21). Thus phase III is the indicator of the replicative end of normal animal cells, because in the case of mouse and rabbit most of the cells after phase III had changed chromosomal constitutions (18,19,21).…”

mentioning

confidence: 99%

Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo

Röhme

1981

Proc. Natl. Acad. Sci. U.S.A.

311

135

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The replicative life spans of mammalian fibroblasts in vitro were studied in a number of cell cultures representing eight species. Emphasis was placed on determining the population doubling level at which phase III (a period of decrease in the rate of proliferation) and chromosomal alterations occur. All the cell cultures studied went through a growth crisis, a period of apparent growth cessation lasting for at least 2 weeks. In most cultures, the crisis represented the end of their replicative capacities, but in some cultures cell proliferation was resumed after the crisis. A predominantly diploid chromosome constitution (more than 75%) was demonstrated prior to the growth crisis. In cultures in which cell proliferation was resumed after the crisis, a nondiploid constitution prevailed in all cases except the rat (with 90% or more diploid cells all the time). The growth crisis occurred at population doubling levels that were characteristic for the species and was shown to be related to the species' maximal life span by a strict power law, being proportional to the square root of the maximal life span. Based on data in the literature, the same relationship was also valid for the lifespans ofcirculating mammalian erythrocytes in vivo. These results may indicate the prevalence of a common functional basis regulating the life span of fibroblasts and erythrocytes and thus operating in replicative as well as postmitotic cells in vitro and in viva

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“…Human arterial smooth muscle cells from donors aged 0-80 years had cultural life-spans of5-15 PDs (Bierman, 1978) and monkey aortic smooth muscle cultures had life-spans of 8-27 PDs (Martin and Sprague, 1973). Human umbilical vein smooth muscle cells had culture life-spans of 1 0 3 0 PDs (Martin and Ogburn, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…In summary, we have presented evidence of cellular senescence in cultured bovine fetal aortic endothelial cells, a system showing both similarities to and differences from diploid fibroblastlike cell cultures. The possible physiologic significance of primary age-related cellular changes in the endothelium has been discussed (Levine and Mueller, 1979;Martin et al, 1975;Martin and Ogburn, 1976). Such changes may contribute to the genesis of atherosclerosis and to the process of tumor invasion and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Proliferative characteristics of clonal endothelial cell strains

Rosen

Mueller

Noveral

et al. 1981

Journal Cellular Physiology

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We have utilized clonal strains of bovine fetal aortic endothelial cells to study cellular senescence in a differentiated cell type of physiological significance. Serial subcultivation of nine endothelial clones derived from three fetal calf aortas revealed proliferative life-spans in vitro of 53--125 population doublings (PDs), compared with 60 and 143 PDs for two lines of bovine fetal lung cells and 85 and 147 PDs for two lines of bovine vascular smooth muscle cells. Serial growth curves showed marked reductions associated with endothelial cellular senescence both in cellular growth rate and culture plateau density. Studies of the 24-hour [3H]-thymidine labeling index versus percentage of proliferative life-span completed indicated that clonal endothelial cultures contained a large proportion (greater than 90%) of rapidly cycling cells until about 75% of the lifespans were completed. Senescent endothelial cells showed evidence of large increases in cell area, cell volume, and protein content. In those clones examined, one specialized endothelial function, Factor VIII antigen expression, was retained qualitatively throughout the life-spans.

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Cell, Tissue, and Organoid Cultures of Blood Vessels

Cited by 6 publications

References 138 publications

Nucleoside requirements for the in vitro growth of bovine aortic endothelial cells

Nucleoside requirements for the in vitro growth of bovine aortic endothelial cells

Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo

Proliferative characteristics of clonal endothelial cell strains

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