Cell Therapy for Acute Myocardial Infarction

Welt, Frederick G.P.; Losordo, Douglas W.

doi:10.1161/circulationaha.105.613034

Cited by 48 publications

(32 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These parameters, however, cannot be readily obtained in the clinical setting in contrast to preclinical animal studies since extensive histologic analysis of myocardial tissue after cell transplantation cannot be performed in patients. Therefore, the development of noninvasive imaging approaches to monitor the fate of implanted stem cells is needed and may help in successful translation of stem cell therapies to patients (18)(19)(20)(21)(22). Several different imaging approaches for visualizing stem cell fate in the heart are currently under investigation, including magnetic resonance (MR) imaging, optical imaging, and PET.…”

Section: Molecular Imaging: Imaging Gene Expression In Human Mesenchymentioning

confidence: 99%

“…Furthermore, conflicting reports on the clinical benefit in patients with coronary artery disease following cell transplantation have raised several questions, including which subpopulation of patients with coronary artery disease may benefit most from which cell type implanted through which delivery approach in the heart (14)(15)(16)(17). The development of objective and quantitative noninvasive (imaging) approaches for tracking stem cell fate, including stem cell location, survival, engraftment, and differentiation, may help in better understanding stem cell biology and may lead to better patient stratification and eventual successful implementation of cardiac stem cell therapy into the clinic (18)(19)(20)(21)(22).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Willmann¹,

Paulmurugan²,

Rodriguez-Porcel³

et al. 2009

Radiology

View full text Add to dashboard Cite

Purpose:To evaluate the feasibility of reporter gene imaging in implanted human mesenchymal stem cells (MSCs) in porcine myocardium by using clinical positron emission tomography (PET)-computed tomography (CT) scanning. Materials and Methods:Animal protocols were approved by the Institutional Administrative Panel on Laboratory Animal Care. Transduction of human MSCs by using different doses of adenovirus that contained a cytomegalovirus (CMV) promoter driving the mutant herpes simplex virus type 1 thymidine kinase reporter gene (Ad-CMV-HSV1-sr39tk) was characterized in a cell culture. A total of 2.25 ϫ 10 6 transduced (n ϭ 5) and control nontransduced (n ϭ 5) human MSCs were injected into the myocardium of 10 rats, and reporter gene expression in human MSCs was visualized with micro-PET by using the radiotracer 9-(4-[fluorine 18]-fluoro-3-hydroxymethylbutyl)-guanine (FHBG). Different numbers of transduced human MSCs suspended in either phosphatebuffered saline (PBS) (n ϭ 4) or matrigel (n ϭ 5) were injected into the myocardium of nine swine, and gene expression was visualized with a clinical PET-CT. For analysis of cell culture experiments, linear regression analyses combined with a t test were performed. To test differences in radiotracer uptake between injected and remote myocardium in both rats and swine, one-sided paired Wilcoxon tests were performed. In swine experiments, a linear regression of radiotracer uptake ratio on the number of injected transduced human MSCs was performed.

show abstract

Section: Molecular Imaging: Imaging Gene Expression In Human Mesenchymentioning

confidence: 99%

mentioning

confidence: 99%

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Willmann¹,

Paulmurugan²,

Rodriguez-Porcel³

et al. 2009

Radiology

View full text Add to dashboard Cite

show abstract

“…The road from vascular biological principles underpinning the promise of cell therapy to an approvable therapy for individuals with CLI will require us to sustain optimism, share data generously, and evaluate risk and benefit diligently. 21 When this is accomplished for CLI, this challenging manifestation of PAD will no longer be an orphan disease for which there is no effective medical treatment.…”

Section: Resultsmentioning

confidence: 99%

Critical Limb Ischemia and Stem Cell Research

Hirsch

2006

Circulation

View full text Add to dashboard Cite

“…They also explained improvement as an endocrine or exocrine effect, or regarded progenitor cells as inflammatory cells when in the myocardium. 17 In the myocardium, monocyte-derived macrophages and mast cells may also produce cytokines and growth factors necessary for fibroblast proliferation and neovascularization, leading to effective repair and scar formation. 18 Generally, circulating mononuclear cells are an important source of inflammatory cytokines in the plasma and myocardium.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory responses after intracoronary mononuclear BM cell therapy in swine

Guo

et al. 2009

Bone Marrow Transplant

View full text Add to dashboard Cite

The aim of this study was to investigate the influence of intracoronary injection of mononuclear BM cells (mBMCs) on inflammatory mediators in swine with acute myocardial infarction (AMI) in two groups. Group 1 received the therapy immediately after AMI, but group 2 received it after 3 weeks. The levels of cytokines and heart functions, respectively, were examined 3 weeks after cell therapy. The serum levels of TNF-a and IL-8 were significantly lower than in the control group: in contrast, IL-10 was significantly elevated in the cell recipients, especially in group 1. TNF-a correlated with IL-8 between groups. The ventricular functions were significantly improved. These results show that although the intracoronary injection of mBMCs induces a marked short-term inflammatory response in the acute stage, mBMCs may play an anti-inflammatory role topically and systematically and early cell therapy may be preferable.

show abstract

Cell Therapy for Acute Myocardial Infarction

Cited by 48 publications

References 40 publications

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Imaging Gene Expression in Human Mesenchymal Stem Cells: From Small to Large Animals

Critical Limb Ischemia and Stem Cell Research

Inflammatory responses after intracoronary mononuclear BM cell therapy in swine

Contact Info

Product

Resources

About