Cell targeted phagemid rescued by preselected landscape phage

Mount, Jane D.; Samoylova, Tatiana I.; Morrison, Nancy E.; Cox, Nancy R.; Baker, Henry J.; Petrenko, Valery A.

doi:10.1016/j.gene.2004.07.006

Cited by 35 publications

(39 citation statements)

References 28 publications

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“…Landscape phages have been shown to serve as substitutes for antibodies against various soluble and cell-displayed antigens and receptors (Legendre and Fastrez, 2002;Mount et al, 2004;Petrenko and Smith, 2000;Petrenko et al, 2002;Romanov et al, 2001;Samoylova et al, 2003;Samoylova et.al., 2004) including bacterial cells and spores (Brigati et al, 2004;Petrenko and Sorokulova, 2004). The phage probes have been used in ELISA and thickness shear mode quartz sensors to detect bacterial and mammalian antigens (Petrenko and Smith, 2000;Petrenko and Vodyanoy, 2003).…”

Section: Salmonella Typhimurium {S Typhimurium) Ranks As a Leading Cmentioning

confidence: 99%

Landscape phage probes for Salmonella typhimurium

Sorokulova

Olsen

Chen

et al. 2005

Journal of Microbiological Methods

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109

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Section: Salmonella Typhimurium {S Typhimurium) Ranks As a Leading Cmentioning

confidence: 99%

Landscape phage probes for Salmonella typhimurium

Sorokulova

Olsen

Chen

et al. 2005

Journal of Microbiological Methods

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109

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“…We attempted to use the ligand sequence from this clone to modify the pIII gene of the filamentous helper phage M13KO7. In a previous study, it was demonstrated that the pVIII of helper phages could be modified by the addition of a peptide (19). Phagemids without the coding sequence of pVIII can be encapsulated by the helper phage.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Peptide-Targeted Phagemid Particles Using a Protein III-Modified Helper Phage

Zhang

Zhao

et al. 2005

BioTechniques

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Ligand or peptide-targeted phagemid particles are being pursued as vehicles for receptor-mediated gene delivery. Here we describe a helper phage in which the protein III (pIII) protein is modified by the addition of a ligand peptide sequence at the amino terminus. Phagemid particles can be prepared with the help of this modified helper phage and should display the ligand peptide in most of the pIII proteins on the phagemid surface. Using such a method, it is not necessary for the phagemid to encode the pIII protein, which leaves a larger space for cloning genes of interest. In addition, the technique should allow for the rapid testing of peptide ligands selected from phage display libraries using phagemids encoding various reporter genes (e.g., green fluorescent protein, luciferase, beta-galactosidase) and therapeutic genes.

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“…Phages can be selected from landscape phage display libraries with affinities for a wide range of simple targets such as dioxin, Cibacron blue, β-galactosidase, streptavidin, neutravidin and fibrinogen [11,13,14,27], as well as for more complex targets such as prostate cancer cells [15,16,28], malignant glial cells [17,25,30], or serum antibodies [31]. Landscape phages have been shown to serve as substitutes for antibodies and detection probes in enzyme-linked immuno assay (ELISA) and biosensors [13][14][15][16][17][18][19][20][21][22][23], as immunogens [24], gene-delivery vehicles [25] and affinity matrices [30,32].…”

Section: Landscape Phages As Substitute Antibodiesmentioning

confidence: 99%

“…Landscape phages have been shown to serve as substitutes for antibodies and detection probes in enzyme-linked immuno assay (ELISA) and biosensors [13][14][15][16][17][18][19][20][21][22][23], as immunogens [24], gene-delivery vehicles [25] and affinity matrices [30,32].…”

Section: Landscape Phages As Substitute Antibodiesmentioning

confidence: 99%

Landscape phage as a molecular recognition interface for detection devices

Petrenko

2008

Microelectronics Journal

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Filamentous phages are thread-shaped bacterial viruses. Their outer coat is a tube formed by thousands equal copies of the major coat protein pVIII. Libraries of random peptides fused to pVIII domains were used for selection of phages probes specific for a panel of test antigens and biological threat agents. Because the viral carrier in the phage borne bio-selective probes is infective, they can be cloned individually and propagated indefinitely without needs of their chemical synthesis or reconstructing. As a new bioselective material, landscape phages combine unique characteristics of affinity reagents and self assembling proteins. Biorecognition layers formed by the phage-derived probes bind biological agents with high affinity and specificity and generate detectable signals in analytical platforms. The performance of phage-derived materials as biorecognition interface was illustrated by detection of Bacillus anthracis spores and Salmonella typhimurium cells. With further refinement, the phage-derived analytical platforms for detecting and monitoring of numerous threat agents may be developed, since phage interface against any bacteria, virus or toxin may be readily selected from the landscape phage libraries. As an interface in the field-use detectors, they may be superior to antibodies, since they are inexpensive, highly specific and strong binders, resistant to high temperatures and environmental stresses.

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Cell targeted phagemid rescued by preselected landscape phage

Cited by 35 publications

References 28 publications

Landscape phage probes for Salmonella typhimurium

Landscape phage probes for Salmonella typhimurium

Preparation of Peptide-Targeted Phagemid Particles Using a Protein III-Modified Helper Phage

Landscape phage as a molecular recognition interface for detection devices

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