Cell-targetable DNA nanocapsules for spatiotemporal release of caged bioactive small molecules

Veetil, Aneesh Tazhe; Chakraborty, Kasturi; Xiao, Kangni; Minter, Myles R.; Sisodia, Sangram S.; Krishnan, Yamuna

doi:10.1038/nnano.2017.159

Cited by 105 publications

(90 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fundamental aspects underlying MISS, such as receptor binding, kinetics of ion-channel opening, and production of downstream effector molecules remain obscure because a pristine molecular technology that could trigger the release of signaling steroids was not available. We have recently described a prototype DNA nanocapsule which can be programmed to release small molecules upon photoirradiation [Veetil AT, et al (2017) Nat Nanotechnol 12:1183-1189]. Here we show that this DNA-based molecular technology can now be programmed to chemically trigger MISS, significantly expanding its applicability to systems that are refractory to photoirradiation.…”

mentioning

confidence: 86%

“…S3 and S4 and Schemes S1-S3. It is known from previous studies that the fluorescence of methoxyfluorescein and 4-MU are effectively quenched when they are derivatized as carbonates (17,19). Thus, in their chemically caged forms, both FLD 10 and CD 10 show extremely weak background fluorescence.…”

Section: Significancementioning

confidence: 99%

“…Particularly, the DNA icosahedron has been used to encapsulate diverse cargo such as gold nanoparticles, fluorescent polymers (15,16), and quantum dots for bioimaging (13). Recently, we have shown that small molecules can be released from DNA icosahedra in response to a light stimulus in transparent organisms (17). Despite being a clean and traceless trigger, light can only be applied to systems that are transparent.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Chemical control over membrane-initiated steroid signaling with a DNA nanocapsule

Veetil

Jani

Krishnan

2018

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Membrane-initiated steroid signaling (MISS) is a recently discovered aspect of steroidal control over cell function that has proved highly challenging to study due to its rapidity and ultrasensitivity to the steroid trigger [Chow RWY, Handelsman DJ, Ng MKC (2010) Endocrinology 151:2411-2422]. Fundamental aspects underlying MISS, such as receptor binding, kinetics of ion-channel opening, and production of downstream effector molecules remain obscure because a pristine molecular technology that could trigger the release of signaling steroids was not available. We have recently described a prototype DNA nanocapsule which can be programmed to release small molecules upon photoirradiation [Veetil AT, et al. (2017) Nat Nanotechnol 12:1183-1189]. Here we show that this DNA-based molecular technology can now be programmed to chemically trigger MISS, significantly expanding its applicability to systems that are refractory to photoirradiation.estradiol | DNA nanotechnology | eNOS | cell signaling | live imaging

show abstract

mentioning

confidence: 86%

Section: Significancementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Chemical control over membrane-initiated steroid signaling with a DNA nanocapsule

Veetil

Jani

Krishnan

2018

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The pH‐sensitive property of fluorescein enabled monitoring of pH changes associated with endosomal maturation. In a subsequent study, photoresponsive polymers attached to small molecule payloads were loaded into DNA icosahedrons . Light‐activated release of the small molecule could be monitored with spatial and temporal precision.…”

Section: Aptamer‐based Probesmentioning

confidence: 99%

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

View full text Add to dashboard Cite

The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.

show abstract

“…15 For large, megadalton-sized structures typically measuring tens to hundreds of nanometers, the DNA origami approach became particularly popular due to its robustness and versatility. [16][17][18] For some applications, smaller structures such as tetrahedra, 19 icosahedra, 20 or structures from DNA minicircles (MCs). [21][22][23][24][25] consisting of fewer 4 peer-reviewed) is the author/funder.…”

Section: -11mentioning

confidence: 99%

DNA-Encircled Lipid Bilayers

Iric

Subramanian²,

Oertel

et al. 2018

Preprint

View full text Add to dashboard Cite

Lipid bilayers and lipid-associated proteins play a crucial role in biology. As in vivo studies and manipulation are inherently difficult, several membrane-mimetic systems have been developed to enable investigation of lipidic phases, lipid-protein interactions, membrane protein function and membrane structure in vitro. Controlling the size and shape, or site-specific functionalization is, however, difficult to achieve with established membrane mimetics based on membrane scaffolding proteins, polymers or peptides. In this work, we describe a route to leverage the unique programmability of DNA nanotechnology and create DNA-encircled bilayers (DEBs), which are made of multiple copies of an alkylated oligonucleotide hybridized to a single-stranded minicircle. To stabilize the hydrophobic rim of the lipid bilayer, and to prevent formation of lipid vesicles, we introduced up to 2 alkyl chains per helical that point to the inside of the toroidal DNA ring and interact with the hydrophobic side chains of the encapsulated lipid bilayer. The DEB approach described herein provides unprecedented control of size, and allows the orthogonal functionalizations and arrangement of engineered membrane nanoparticles and will become a valuable tool for biophysical investigation of lipid phases and lipid-associated proteins and complexes including structure determination of membrane proteins and pharmacological screenings of membrane proteins.

show abstract

Cell-targetable DNA nanocapsules for spatiotemporal release of caged bioactive small molecules

Cited by 105 publications

References 33 publications

Chemical control over membrane-initiated steroid signaling with a DNA nanocapsule

Chemical control over membrane-initiated steroid signaling with a DNA nanocapsule

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

DNA-Encircled Lipid Bilayers

Contact Info

Product

Resources

About