Cell Tagging with Clinically Approved Iron Oxides: Feasibility and Effect of Lipofection, Particle Size, and Surface Coating on Labeling Efficiency

Matuszewski, Lars; Persigehl, Thorsten; Wall, Alexander; Schwindt, Wolfram; Tombach, Bernd; Fobker, Manfred; Poremba, Christopher; Ebert, Wolfgang; Heindel, Walter; Bremer, Christoph

doi:10.1148/radiol.2351040094

Cited by 173 publications

(166 citation statements)

References 42 publications

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“…For example, carboxydextran-coated SPIO (of equal or even smaller sizes) appear to be internalized by macrophages to a greater extent than dextran-coated SPIO. 90 It should be noted, however, that uptake of SPIO by macrophages is not associated with cell activation as no interleukine-1 release is observed. 116 When used as a contrast agent in living subjects, adverse events from USPIO (dextran-coated SPIO) were noted as not serious, mild in severity, and short in duration.…”

Section: Biocompatibilitymentioning

confidence: 95%

“…124 It should be noted that the efficiency of SPIO uptake by macrophage and MR contrast is highly dependent on both circulation time and surface charge. 90 Typically, in vivo, smaller nanoparticles (15-30 nm hydrodynamic diameter) with longer circulation times exhibit improved contrast at sites of inflammation compared with larger nanoparticles. 53 The role of macrophages in pathologic tissue alterations in the central nervous system (CNS) has also provided the opportunity for the use of SPIO agents for the imaging of strokes 127 (Fig.…”

Section: Macrophage Imagingmentioning

confidence: 99%

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Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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Abstract-The field of molecular imaging has recently seen rapid advances in the development of novel contrast agents and the implementation of insightful approaches to monitor biological processes non-invasively. In particular, superparamagnetic iron oxide nanoparticles (SPIO) have demonstrated their utility as an important tool for enhancing magnetic resonance contrast, allowing researchers to monitor not only anatomical changes, but physiological and molecular changes as well. Applications have ranged from detecting inflammatory diseases via the accumulation of non-targeted SPIO in infiltrating macrophages to the specific identification of cell surface markers expressed on tumors. In this article, we attempt to illustrate the broad utility of SPIO in molecular imaging, including some of the recent developments, such as the transformation of SPIO into an activatable probe termed the magnetic relaxation switch.

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Section: Biocompatibilitymentioning

confidence: 95%

Section: Macrophage Imagingmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

et al. 2006

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“…For inorganic-based NPs, similar results were reported. Some researchers reported an optimal particle size for uptake of Au NPs, CNTs, and MSNs ($50 nm) [6,[39][40][41], but some others found smaller Au, silica, and IO NPs had improved cellular uptake than larger ones in a certain size range [42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

Dong

Parekh

2015

Journal of Colloid and Interface Science

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a b s t r a c tIt is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake; (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value; and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.

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“…The use of an HIV-1 transactivator (Tat) peptide sequence, for example, can increase their uptake by lymphocytes more than 100-fold compared to untagged nanoparticles (21,22), permitting in vivo tracking of labeled cells (23)(24)(25). Other approaches to increase cellular uptake include receptor-mediated endocytosis, the use of carrier transporters as magnetodendrimers (26,27), and transfection methods combined with simple incubation (28,29).…”

mentioning

confidence: 99%

In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy

Смирнов

Lavergne

Gazeau

et al. 2006

Magnetic Resonance in Med

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Cell Tagging with Clinically Approved Iron Oxides: Feasibility and Effect of Lipofection, Particle Size, and Surface Coating on Labeling Efficiency

Abstract: Lipofection-based cell tagging is a simple method for efficient cell labeling with clinically approved iron oxide-based contrast agents. Large particle size and carboxydextran coating are preferable for cell tagging with endocytosis- and lipofection-based methods.

Cited by 173 publications

References 42 publications

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging

Particle size- and number-dependent delivery to cells by layered double hydroxide nanoparticles

In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy

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