Cell suspension culture extract of Eriobotrya japonica attenuates growth and induces apoptosis in prostate cancer cells via targeting SREBP-1/FASN-driven metabolism and AR

Hsieh, Po Fan; Jiang, Wenping; Basavaraj, Praveenkumar; Huang, Shu‐Mei; Ruangsai, Phakkhathorn; Wu, Jin‐Bin; Huang, Guan-Jhong; Huang, Wen‐Chin

doi:10.1016/j.phymed.2021.153806

Cited by 11 publications

(6 citation statements)

References 34 publications

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“…The results from the in vitro and in vivo experiments demonstrated that the ethanol extract of Davallia formosana can suppress proliferation, migration, and invasion in PCa cells by inhibiting the levels of SREBP-1, FASN, AR, and PSA, suggesting that SREBP-1/FASN/lipogenesis and the AR axis could be potential targets for the treatment of PCa ( 173 ). The cell suspension culture extract from Eriobotrya japonica significantly inhibits PCa cell growth, migration, and invasion by decreasing the SREBP-1/FASN-mediated lipid metabolism and AR signaling pathway in the cell and mouse models ( 174 ). Moreover, the polysaccharides from Astragalus membranaceus (APS) greatly inhibit the proliferation and invasion of PCa cells in a dose-dependent and time-dependent manner.…”

Section: Targeting the Srebp-1 Signaling Pathway For Cancer Therapymentioning

confidence: 99%

Targeting SREBP-1-Mediated Lipogenesis as Potential Strategies for Cancer

2022

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Sterol regulatory element binding protein-1 (SREBP-1), a transcription factor with a basic helix–loop–helix leucine zipper, has two isoforms, SREBP-1a and SREBP-1c, derived from the same gene for regulating the genes of lipogenesis, including acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase. Importantly, SREBP-1 participates in metabolic reprogramming of various cancers and has been a biomarker for the prognosis or drug efficacy for the patients with cancer. In this review, we first introduced the structure, activation, and key upstream signaling pathway of SREBP-1. Then, the potential targets and molecular mechanisms of SREBP-1-regulated lipogenesis in various types of cancer, such as colorectal, prostate, breast, and hepatocellular cancer, were summarized. We also discussed potential therapies targeting the SREBP-1-regulated pathway by small molecules, natural products, or the extracts of herbs against tumor progression. This review could provide new insights in understanding advanced findings about SREBP-1-mediated lipogenesis in cancer and its potential as a target for cancer therapeutics.

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Section: Targeting the Srebp-1 Signaling Pathway For Cancer Therapymentioning

confidence: 99%

Targeting SREBP-1-Mediated Lipogenesis as Potential Strategies for Cancer

2022

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“…Validation of this flow cytometric approach was accomplished with appropriate specificity, accuracy, and sensitivity for all compounds, and it is thus possible to detect and identify drug-induced apoptosis in the 3D bioprinted hydrogel scaffolds. In order to study whether the anti-cancer drug etoposide caused cell death and apoptosis, an Annexin V/PI staining technique was chosen as it has been used widely for various apoptosis detection and drug efficacy studies [64][65][66]. Annexin V is a protein that can adhere to PS translocated to the cell membrane's outer surface, and can be used to observe apoptotic cells by marking it with a fluorescent chemical (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Because this protein binding may be recognized on the surface of necrotic cells, a second non-vital dye PI is added to enable the simultaneous detection of two distinct cells [63]. Annexin V and PI have been used widely for various apoptosis detection and drug efficacy studies [64][65][66]. A375 melanoma cells cultured in 2D and cultured in the 3D in the alginate-gelatine hydrogel scaffolds were treated with different concentrations of etoposide for 4 h and then stained and analysed.…”

Section: Anti-cancer Activity Of Etoposide On 3d Bioprinted A375 Mela...mentioning

confidence: 99%

Flow cytometry as an analytical method of drug-induced apoptosis in 3D bioprinted melanoma cells

Villiers

Plessis

2023

Biomed. Mater.

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Three-dimensional (3D) cell culture systems have gained increasing interest in drug discovery and tissue engineering due to its inherent advantages in providing more physiologically relevant information and more predictive data for in vivo tests. Along with the development of more physiologically relevant 3D cell culture models, researchers bear the responsibility to validate new cell assay techniques capable of measuring and evaluating constructs that are physically larger and more complex compared to two- dimensional (2D) cell cultures. It is important to note that assays based on monolayer cultures may be insufficient for the use in 3D cell cultures models. In this study we firstly fabricated a 3D bioprinted hydrogel melanoma scaffold. This was used to validate a flow cytometry-based analytical method as a tool for 3D bioprinted structures to assess drug-induced apoptosis. The results indicated high robustness, reproducibility and sensitivity of the flow cytometric method established on the 3D cell-laden A375 melanoma hydrogel scaffolds. Over and above this, it was possible to determine the effect of etoposide on A375 melanoma cells using Annexin V and PI apoptosis assay.

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“…This in turn can promote PCa growth, migration, invasion and depot resistance (36,56) and is positively associated with the clinical Gleason classification of human PCa (56). SREBP-1/FASN inhibition reduces fatty acid levels and lipid droplet accumulation in PCa cells (57). In healthy cells, there are two proteins SREBP-1, SREBP-1a and SREBP-1c, the latter of which is involved in controlling the ab initio production of endogenous fatty acids (55).…”

Section: Sources Of Fatty Acidsmentioning

confidence: 99%

New insights into lipid metabolism and prostate cancer (Review)

et al. 2023

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Prostate cancer (PCa) is the most common malignant tumor of the male urological system and poses a severe threat to the survival of middle-aged and elderly males worldwide. The development and progression of PCa are affected by a variety of biological processes, including proliferation, apoptosis, migration, invasion and the maintenance of membrane homeostasis of PCa cells. The present review summarizes recent research advances in lipid (fatty acid, cholesterol and phospholipid) metabolic pathways in PCa. In the first section, the metabolism of fatty acids is highlighted, from formation to catabolism and associated proteins. Subsequently, the role of cholesterol in the pathogenesis and evolution of PCa is described in detail. Finally, the different types of phospholipids and their association with PCa progression is also discussed. In addition to the impact of key proteins of lipid metabolism on PCa growth, metastasis and drug resistance, the present review also summarizes the clinical value of fatty acids, cholesterol and phospholipids, as diagnostic and prognostic indicators and therapeutic targets in PCa. Contents1. Introduction 2. Fatty acid metabolism in prostate cancer 3. Cholesterol and its ester metabolism in prostate cancer 4. Phospholipid metabolism in prostate cancer 5. Lipid metabolism and the diagnosis and treatment of prostate cancer 6. Conclusions and future perspectives

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Cell suspension culture extract of Eriobotrya japonica attenuates growth and induces apoptosis in prostate cancer cells via targeting SREBP-1/FASN-driven metabolism and AR

Cited by 11 publications

References 34 publications

Targeting SREBP-1-Mediated Lipogenesis as Potential Strategies for Cancer

Targeting SREBP-1-Mediated Lipogenesis as Potential Strategies for Cancer

Flow cytometry as an analytical method of drug-induced apoptosis in 3D bioprinted melanoma cells

New insights into lipid metabolism and prostate cancer (Review)

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