Cell surface shaving of <i><scp>C</scp>andida albicans</i> biofilms, hyphae, and yeast form cells

Vialás, Vital; Palani, P.; Gutiérrez, Dolores; Ximénez-Embún, Pilar; Nombela, César; Gil, Concha; Chaffin, W. LaJean

doi:10.1002/pmic.201100588

Cited by 53 publications

(58 citation statements)

References 23 publications

(48 reference statements)

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“…Likewise, the profiling of C. albicans surface proteins was reported by two other groups in different analytical approaches [29, 32]. Broadly speaking, similar functions were observed in Tables 1 and 2.…”

Section: Resultssupporting

confidence: 73%

Miniaturized Digestion and Extraction of Surface Proteins fromCandida albicansfollowing Treatment with Histatin 5 for Mass Spectrometry Analysis

Fan

Moffa

Xiao

et al. 2016

International Journal of Proteomics

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A common approach to isolate surface proteins from fungal and bacterial cells is to perform a proteolytic cleavage of proteins on the surface of intact cells suspended in solution. This paper describes miniaturization of this technique, in which cells are adhered on glass surfaces, and all sample treatments are conducted at μL volumes. Specifically, Candida albicans cells were attached onto HSA-coated glass slides. By depositing the appropriate reagent solutions on the adhered cells, we successfully performed cell washing, treatment with antifugal peptide, Histatin 5, and a proteolysis on intact cells with trypsin. The resulting peptides were subsequently analysed by mass spectrometry. In general, the data obtained was similar to that collected with suspended cells in much larger sample volumes. However, our miniaturized workflow offers the benefit of greatly reducing the consumption of cells and reagents.

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Section: Resultssupporting

confidence: 73%

Miniaturized Digestion and Extraction of Surface Proteins fromCandida albicansfollowing Treatment with Histatin 5 for Mass Spectrometry Analysis

Fan

Moffa

Xiao

et al. 2016

International Journal of Proteomics

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“…The cell wall, as the outermost cell structure represents the contact surface with host cells and therefore gathers many antigens, virulence factors and Pathogen Associated Molecular Patterns (PAMPs) [40]. Proteins involved in hyphal growth are also relevant in pathogenesis, in the sense that hyphae have been proven key for invasiveness whereas the switch back to yeast form plays a role in dissemination [41].…”

Section: Resultsmentioning

confidence: 99%

A Candida albicans PeptideAtlas

Vialás

Sun

Penha

et al. 2014

Journal of Proteomics

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Candida albicans public proteomic data sets, though growing steadily in the last few years, still have a very limited presence in online repositories. We report here the creation of a C. albicans PeptideAtlas comprising near 22000 distinct peptides at a 0.24 % False Discovery Rate (FDR) that account for over 2500 canonical proteins at a 1.2% FDR. Based on data from 16 experiments, we attained coverage of 41% of the C.albicans open reading frame sequences (ORFs) in the database used for the searches. This PeptideAtlas provides several useful features, including comprehensive protein and peptide-centered search capabilities and visualization tools that establish a solid basis for the study of basic biological mechanisms key to virulence and pathogenesis such as dimorphism, adherence, and apoptosis. Further, it is a valuable resource for the selection of candidate proteotypic peptides for targeted proteomic experiments via selected reaction monitoring (SRM) or SWATH-MS.

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“…Studies describing the transcriptome, proteome and lipidomics of developing C. albicans biofilms as well as comparative analyses of C. albicans biofilms with planktonically growing cells have improved our understanding of the resistance and pathogenicity traits of the biofilm lifestyle. Distinguished lipid and protein (secreted, cell wall-associated and complete protein fractions) profiles have been observed between developing biofilms and planktonic cells [13][14][15][16][17]. Also transcriptional studies revealed time-dependent changes in global gene expression between different stages of biofilm development.…”

Section: Pathogenesismentioning

confidence: 99%

Combinatorial drug approaches to tackleCandida albicansbiofilms

Cremer

Staes

Delattin

et al. 2015

Expert Review of Anti-infective Therapy

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The human fungal opportunistic pathogen Candida albicans resides in the human gut, genitourinary tract and on the skin. The majority of infections caused by C. albicans are biofilm-related. In the first part of this review, we discuss new insights into C. albicans biofilm characteristics, concentrating on the extracellular matrix, phenotypic switching, efflux pumps and persister cells. It is widely accepted that this multicellular lifestyle is more resistant to traditional antifungal treatment compared to free-living cells. Therefore, much effort is put in the search for combinations of drugs leading to synergistic interactions against microbial biofilms to achieve lower effective doses of the drugs. In the second part of this manuscript, we review all recently identified compounds that act synergistically with azoles, echinocandins and/or polyenes against C. albicans biofilms.

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Cell surface shaving of Candida albicans biofilms, hyphae, and yeast form cells

Cited by 53 publications

References 23 publications

Miniaturized Digestion and Extraction of Surface Proteins fromCandida albicansfollowing Treatment with Histatin 5 for Mass Spectrometry Analysis

Miniaturized Digestion and Extraction of Surface Proteins fromCandida albicansfollowing Treatment with Histatin 5 for Mass Spectrometry Analysis

A Candida albicans PeptideAtlas

Combinatorial drug approaches to tackleCandida albicansbiofilms

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