Cell surface properties of rhizobial strains isolated from soils contaminated with hydrocarbons: hydrophobicity and adhesion to sandy soil

Mehmannavaz, Reza; Prasher, Shiv O.; Ahmad, Darakhshan

doi:10.1016/s0032-9592(00)00266-1

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…During transport through the soil, the bacteria can lose viability due to nutrient limitation, predation, cell lysis or parasitism (9). Bacterial hydrophobicity has been cited as a criterion to select strains for injection in porous matrices, since it will determine which strain will remain suspended for a long period, facilitating its transport into the porous medium (21). Besides, the cells may adsorb to soil surfaces, making their transport difficult through a porous matrix.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen starvation affects bacterial adhesion to soil

Borges¹,

Nascimento²,

Rocha³

et al. 2008

Braz. J. Microbiol.

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One of the main factors limiting the bioremediation of subsoil environments based on bioaugmentation is the transport of selected microorganisms to the contaminated zones. The characterization of the physiological responses of the inoculated microorganisms to starvation, especially the evaluation of characteristics that affect the adhesion of the cells to soil particles, is fundamental to anticipate the success or failure of bioaugmentation. The objective of this study was to investigate the effect of nitrogen starvation on cell surface hydrophobicity and cell adhesion to soil particles by bacterial strains previously characterized as able to use benzene, toluene or xilenes as carbon and energy sources. The strains LBBMA 18-T (non-identified), Arthrobacter aurescens LBBMA 98, Arthrobacter oxydans LBBMA 201, and Klebsiella sp. LBBMA 204–1 were used in the experiments. Cultivation of the cells in nitrogen-deficient medium caused a significant reduction of the adhesion to soil particles by all the four strains. Nitrogen starvation also reduced significantly the strength of cell adhesion to the soil particles, except for Klebsiella sp. LBBMA 204–1. Two of the four strains showed significant reduction in cell surface hydrophobicity. It is inferred that the efficiency of bacterial transport through soils might be potentially increased by nitrogen starvation.

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Section: Introductionmentioning

confidence: 99%

Nitrogen starvation affects bacterial adhesion to soil

Borges¹,

Nascimento²,

Rocha³

et al. 2008

Braz. J. Microbiol.

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“…The capacity of some species to adhere to soil particles more strongly than others (Mehmannavaz et al 2001) will affect their ease of extraction. However, even if large and representative numbers are extracted, their culturability and, therefore, their enumeration will be determined by the nutritional and growth requirements of the individual species.…”

Section: Discussionmentioning

confidence: 99%

Seasonal variation of different microorganisms with nickel and cadmium in the industrial wastewater and agricultural soils

Ansari

Malik

2009

Environ Monit Assess

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Wastewater and soil samples were collected from the industrial area of Ghaziabad City, India from January 2005 to December 2007 and were analyzed for the presence of heavy metals by atomic absorption spectrophotometry. Test samples revealed high levels of Fe, Cr, Cu, Ni, Zn, and Cd as 967.03, 34.63, 27.97, 19.7, 16.70, and 3.20 mg/L of wastewater, respectively. The concentrations of inorganic minerals were higher in the soil samples irrigated with wastewater. Total coliforms were found to be maximum (1,133x10(4) most probable number per 100 mL) during spring and summer followed by winter and postmonsoon in the wastewater samples. The microbial count in soil as well as in wastewater decreases as the metal concentration increases. The concentration 200 microg/mL of nickel and cadmium inhibits majority of the population, while, at some points, it inhibits 100% of the population. The exponential decay model for microbial count at the increasing metal concentrations indicate that asymbiotic N2 fixers were best fitted to the model. In all the seasons, the order of decline in terms of exponential decay of the population of different microbial groups in soil was asymbiotic N2 fixers>actinomycetes>fungi>aerobic heterotrophic bacteria. The different microbial groups that have different values of slope in different seasons indicate that the resistant population of microorganisms was variable with seasons.

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“…Passive FBC004 movements by diffusion may nevertheless have contributed to the spatial spreading observed after benzoate percolation and long-term incubation. Bacterial growth occurred during the benzoate percolation, and it is known that actively growing soil bacteria are less firmly attached to soil particles than are the remainder of the bacterial community (18), probably because of their cell surface properties (1,28), and could thus disperse more easily than nondividing cells. In the case of long-term incubations, another mechanism may explain spatial spreading by passive movement: in soil, a reduction in the length of introduced P. fluorescens cells has been observed, probably due to the stress caused by nutrient scarcity (50).…”

Section: Discussionmentioning

confidence: 99%

Impact of the Microscale Distribution of a Pseudomonas Strain Introduced into Soil on Potential Contacts with Indigenous Bacteria

Dechesne

Pallud

Bertolla

et al. 2005

Appl Environ Microbiol

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Soil bioaugmentation is a promising approach in soil bioremediation and agriculture. Nevertheless, our knowledge of the fate and activity of introduced bacteria in soil and thus of their impact on the soil environment is still limited. The microscale spatial distribution of introduced bacteria has rarely been studied, although it determines the encounter probability between introduced cells and any components of the soil ecosystem and thus plays a role in the ecology of introduced bacteria. For example, conjugal gene transfer from introduced bacteria to indigenous bacteria requires cell-to-cell contact, the probability of which depends on their spatial distribution. To quantitatively characterize the microscale distribution of an introduced bacterial population and its dynamics, a gfp-tagged derivative of Pseudomonas putida KT2440 was introduced by percolation in repacked soil columns. Initially, the introduced population was less widely spread at the microscale level than two model indigenous functional communities: the 2,4-dichlorophenoxyacetic acid degraders and the nitrifiers (each at 10 6 CFU g ؊1 soil). When the soil was percolated with a substrate metabolizable by P. putida or incubated for 1 month, the microscale distribution of introduced bacteria was modified towards a more widely dispersed distribution. The quantitative data indicate that the microscale spatial distribution of an introduced strain may strongly limit its contacts with the members of an indigenous bacterial community. This could constitute an explanation to the low number of indigenous transconjugants found most of time when a plasmid-donor strain is introduced into soil.

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Cell surface properties of rhizobial strains isolated from soils contaminated with hydrocarbons: hydrophobicity and adhesion to sandy soil

Cited by 13 publications

References 27 publications

Nitrogen starvation affects bacterial adhesion to soil

Nitrogen starvation affects bacterial adhesion to soil

Seasonal variation of different microorganisms with nickel and cadmium in the industrial wastewater and agricultural soils

Impact of the Microscale Distribution of a Pseudomonas Strain Introduced into Soil on Potential Contacts with Indigenous Bacteria

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