Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12

Aono, Rikizo; Kobayashi, Hideki

doi:10.1128/aem.63.9.3637-3642.1997

Cited by 128 publications

(53 citation statements)

References 33 publications

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“…the increased hydrophilicity would repulse butanol avoiding its penetration into the cell. This cells' response is consistent with a previous finding in E. coli K-12 mutants, which had a cell surface less hydrophobic compared to the parental cells, and displayed higher tolerance to organic solvents [28]. Similar adaptation mechanism to the toxic effects of organic solvents was described by Torres et al…”

Section: Natural Shift Of Csh Of Lab Strains In Butanol Environmentsupporting

confidence: 92%

Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria

Petrova

Tsvetanova

Petrov

2018

Engineering in Life Sciences

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Highly butanol‐tolerant strains have always been attractive because of their potential as microbial hosts for butanol production. However, due to the amphiphilic nature of 1‐butanol as a solvent, the relationship between the cell surface hydrophobicity and butanol resistance remained ambiguous to date. In this work, the quantitatively estimated cell surface hydrophobicity of 74 Lactic acid bacteria strains were juxtaposed to their tolerance to various butanol concentrations. The obtained results revealed that the strains’ hydrophobicity was inversely proportional to their butanol tolerance. All highly butanol‐resistant strains were hydrophilic (cell surface hydrophobicity<1%), whereas the more hydrophobic the strains were, the more sensitive to butanol they were. Furthermore, cultivation at increasing butanol concentrations showed a clear tendency to decrease the level of hydrophobicity in all tested organisms, thus suggesting possible adaptation mechanisms. Purposeful reduction of cell surface hydrophobicity (by removal of S‐layer proteins from the cell envelope) also led to an increase of butanol resistance. Since the results covered 23 different Lactic acid bacteria species of seven genera, it could be concluded that regardless of the species, the lower degree of cells’ hydrophobicity clearly correlates with the higher level of butanol tolerance.

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Section: Natural Shift Of Csh Of Lab Strains In Butanol Environmentsupporting

confidence: 92%

Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria

Petrova

Tsvetanova

Petrov

2018

Engineering in Life Sciences

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“…Pseudomonas aeruginosa PseA cells of log phase cultivated in the absence and presence of solvents at 30°C were centrifuged at 8000 g and resuspended in saline (0AE8% NaCl) to A 660 = 0AE6. The suspension was analysed by the MATH test as described by Aono and Kobayashi (1997). H e p t a n e ( 4 · 0 ) I s o o c t a n e ( 4 · 5 ) D o d e c a n e ( 6 · 6 ) T e t r a d e c a n e ( 7 · 6 ) Solvents…”

Section: Microbial Adhesion To Hydrocarbon (Math) Testmentioning

confidence: 99%

Cellular response mechanisms inPseudomonas aeruginosaPseA during growth in organic solvents

Gaur¹,

Khare²

2009

Letters in Applied Microbiology

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Aims: Solvent‐tolerant bacteria have emerged as a new class of micro‐organisms able to grow at high concentrations of toxic solvents. Such bacteria and their solvent‐stable enzymes are perceived to be useful for biotransformations in nonaqueous media. In the present study, the solvent‐responsive features of a lipase–producing, solvent‐tolerant strain Pseudomonas aeruginosa PseA have been investigated to understand the cellular mechanisms followed under solvent‐rich conditions. Methods and Results: The solvents, cyclohexane and tetradecane with differing log P‐values (3·2 and 7·6 respectively), have been used as model systems. Effect of solvents on (i) the cell morphology and structure (ii) surface hydrophobicity and (iii) permeability of cell membrane have been examined using transmission electron microscopy, atomic force microscopy and other biochemical techniques. The results show that (i) less hydrophobic (low log P‐value) solvent cyclohexane alters the cell membrane integrity and (ii) cells adapt to organic solvents by changing morphology, size, permeability and surface hydrophobicity. However, no such changes were observed in the cells grown in tetradecane. Conclusions: It may be concluded that P. aeruginosa PseA responds differently to solvents of different hydrophobicities. Bacterial cell membrane is more permeable to less hydrophobic solvents that eventually accumulate in the cytoplasm, while highly hydrophobic solvents have lesser tendency to access the membrane. Significance and Impact of the Study: To the best of our knowledge, these are first time observations that show that way of bacterial solvent adaptability depends on nature of solvent. Difference in cellular responses towards solvents of varying log P‐values (hydrophobicity) might prove useful to search for a suitable solvent for carrying out whole‐cell biocatalysis.

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“…The presence of divalent cations (e.g. Mg 2þ and Ca 2þ ions) was found to improve survival when added to the growth medium supplemented with organic solvents in several P. putida strains (Inoue et al, 1991;Ramos et al, 1995;Weber and de Bont, 1996). It is likely that the divalent cations electrostatically linked adjacent polyanionic LPS molecules and reduced charge repulsion.…”

Section: Alterations In Other Membrane Components In Response To Orgamentioning

confidence: 99%

“…(i) A number of microorganisms tolerant to different organic solvents cannot metabolize the toxic compound, e.g. Escherichia coli strains tolerant to 1% (v/v) hexane do not use (or biotransform) this compound at all (Aono et al, 1991). Furthermore, a number of Pseudomonas strains tolerant to supersaturating concentrations of toluene did not use this compound as a carbon source (Inoue and Horikoshi, 1989).…”

Section: Introductionmentioning

confidence: 99%

Multiple responses of Gram‐negative bacteria to organic solvents

Segura

Duque

Mosqueda

et al. 1999

Environmental Microbiology

114

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Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12

Cited by 128 publications

References 33 publications

Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria

Low cell surface hydrophobicity is one of the key factors for high butanol tolerance of Lactic acid bacteria

Cellular response mechanisms inPseudomonas aeruginosaPseA during growth in organic solvents

Multiple responses of Gram‐negative bacteria to organic solvents

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