Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of <i>Bacillus subtilis</i>

Chung, Young Sup; Breidt, Fred; Dubnau, David

doi:10.1046/j.1365-2958.1998.00989.x

Cited by 81 publications

(88 citation statements)

References 44 publications

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“…A comparable result was obtained for bdbD and bdbC in transcript profiling experiments with DNA arrays. 5 These observations show that ComK is a positive regulator of the bdbDC operon and that the bdbD and bdbC genes can be regarded as late competence genes. 4 Z. Pragai, personal communication.…”

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 87%

“…6, B and D). An unprocessed, membrane-associated ComGG band is usually detectable (5) and is visible in Fig. 6D.…”

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 96%

“…Since very few transformants were obtained when the bdbDC operon was inactivated, this pilin-like protein is an excellent candidate for a BdbD/BdbC substrate. ComGG, another essential, pilin-like competence protein, contains an intermolecular disulfide bond (5). However, only a minor fraction of ComGG is in this disulfide-bonded, dimerized form.…”

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 99%

“…Pre-ComGC, which contains a single predicted membrane-spanning segment, exists as an integral membrane protein with its C terminus facing the cell wall. Upon processing by the ComC signal peptidase, the mature form of ComGC is liberated from the membrane and found in the protoplast supernatant fraction (5,31). In contrast, some pre-ComGG molecules are present as integral membrane proteins, arranged with their C terminus in the cytosol, while other preComGG molecules are peripheral membrane proteins, exposed on the cytosolic face of the membrane.…”

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 99%

“…Instead, the pilin-like ComG proteins, which are translocated to the cell wall and the exterior surface of the membrane after processing, appear to permit contact between transforming DNA and the membrane-localized DNA receptor ComEA (4). It has been shown that ComGC contains an intramolecular disulfide bond and that a minor fraction of ComGG molecules exist as dimers, stabilized by intermolecular disulfide bonds (5). The expression of the genes encoding these transformation proteins is regulated by a complex signal transduction mechanism that culminates in the synthesis of ComK, a factor required for the transcription of the DNA transport genes (6).…”

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confidence: 99%

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The bdbDC Operon of Bacillus subtilisEncodes Thiol-disulfide Oxidoreductases Required for Competence Development

Meima¹,

Eschevins²,

Fillinger³

et al. 2002

Journal of Biological Chemistry

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The development of genetic competence in the Grampositive eubacterium Bacillus subtilis is a complex postexponential process. Here we describe a new bicistronic operon, bdbDC, required for competence development, which was identified by the B. subtilis Systematic Gene Function Analysis program. Inactivation of either the bdbC or bdbD genes of this operon results in the loss of transformability without affecting recombination or the synthesis of ComK, the competence transcription factor. BdbC and BdbD are orthologs of enzymes known to be involved in extracytoplasmic disulfide bond formation. Consistent with this, BdbC and BdbD are needed for the secretion of the Escherichia coli disulfide bond-containing alkaline phosphatase, PhoA, by B. subtilis. Similarly, the amount of the disulfide bond-containing competence protein ComGC is severely reduced in bdbC or bdbD mutants. In contrast, the amounts of the competence proteins ComGA and ComEA remain unaffected by bdbDC mutations. Taken together, these observations imply that in the absence of either BdbC or BdbD, ComGC is unstable and that BdbC and BdbD catalyze the formation of disulfide bonds that are essential for the DNA binding and uptake machinery.

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Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 87%

“…6, B and D). An unprocessed, membrane-associated ComGG band is usually detectable (5) and is visible in Fig. 6D.…”

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 96%

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 99%

Section: Bdbc and Bdbd Are Both Required For The Stability Of The Pilmentioning

confidence: 99%

mentioning

confidence: 99%

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The bdbDC Operon of Bacillus subtilisEncodes Thiol-disulfide Oxidoreductases Required for Competence Development

Meima¹,

Eschevins²,

Fillinger³

et al. 2002

Journal of Biological Chemistry

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Uptake of extracellular DNA: Competence induced pili in natural transformation of Streptococcus pneumoniae

et al. 2015

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Transport of DNA across bacterial membranes involves complex DNA uptake systems. In Gram-positive bacteria, the DNA uptake machinery shares fundamental similarities with type IV pili and type II secretion systems. Although dedicated pilus structures, such as type IV pili in Gram-negative bacteria, are necessary for efficient DNA uptake, the role of similar structures in Gram-positive bacteria is just beginning to emerge. Recently two essentially very different pilus structures composed of the same major pilin protein ComGC were proposed to be involved in transformation of the Gram-positive bacterium Streptococcus pneumoniae – one is a long, thin, type IV pilus-like fiber with DNA binding capacity and the other one is a pilus structure that was thicker, much shorter and not able to bind DNA. Here we discuss how competence induced pili, either by pilus retraction or by a transient pilus-related opening in the cell wall, may mediate DNA uptake in S. pneumoniae.

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Novel Frontiers in the Production of Compounds for Biomedical Use

Broekhoven¹,

Shapiro²,

Anne³

2002

Focus on Biotechnology

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COLOPHON Focus onBiotechnology is an open-ended series of reference volumes produced for Kluwer Academic Publishers BV in co-operation with the Branche Belge de la Société de Chimie Industrielle a.s.b.l.The initiative has been taken in conjunction with the Ninth European Congress on Biotechnology.The present book entitled "Novel Frontiers in the Production of Compounds for Biomedical Uses" can perhaps be placed in its best perspective by the Shakespearean character in The Tempest who exclaimed" What's past is prologue". Indeed, this compilation of some of the outstanding presentations in the field of biomedicine made at the 9 th European Congress on Biotechnology (Brussels, Belgium, July 11-15, 1999) not only reflects the achievements of the recent past, but provides a privileged glimpse of the biotechnology that is emerging in the first decade of the new Millennium.It is becoming increasingly apparent that biotechnology is offering biomedicine novel approaches and solutions to develop a sorely needed new generation of biopharmaceuticals. This is all the more necessary because in recent years, new diseases have emerged with extraordinary lethality in all corners of the globe, while age-related chronic illnesses have filled the gap wherever biomedicine has made successful inroads. The rise of antibiotic resistance also poses major threats to public health. Thus, as disease patterns evolve, the rational development of new drugs is becoming urgent, not only for the clinical outcome of patients, but also in optimising the allocation of scarce health care resources through the use of cost-effective productions methods. It is in response to all these challenges that biotechnology offers new strategies that go beyond the more traditional approaches.By the mid-1990's, the number of recombinant products approved annually for therapeutic use reached double digits. With the advent of the genomics revolution. coupled with proteomics, bioinformatics, high-throughput screening, and microelectronics, the number of potential bioproducts entering clinical trials will surely grow almost exponentially. The improved availability of structural and functional biochemical information, together with rational drug design, will also help fill the drug development pipeline. And it should certainly not be forgotten that the increasing demand for recombinant compounds necessitates improved production systems.In order to better focus on the challenges at hand, the Editors have striven to adopt an integrative approach by selecting contributions linking genomics with bacterial resistance, antibiotics with improved production strategies, optimised production approaches with chemical development programs, chemical modifications with new antibody derivatives and biomaterials, the relation between bioartificial organs and xenotransplantation, apoptosis and process biotechnology, etc. It should be readily apparent from these chapters that the development of truly novel compounds for biomedical use not only requires leading-edge science and technolog...

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Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis

Cited by 81 publications

References 44 publications

The bdbDC Operon of Bacillus subtilisEncodes Thiol-disulfide Oxidoreductases Required for Competence Development

The bdbDC Operon of Bacillus subtilisEncodes Thiol-disulfide Oxidoreductases Required for Competence Development

Uptake of extracellular DNA: Competence induced pili in natural transformation of Streptococcus pneumoniae

Novel Frontiers in the Production of Compounds for Biomedical Use

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