Cell surface hydrophobicity: a predictor of biofilm production in Candida isolates?

Borghi, Elisa; Sciota, Rita; Biassoni, Caterina; Cirasola, Daniela; Cappelletti, Laura; Vizzini, Loredana; Boracchi, Patrizia; Morace, Giulia

doi:10.1099/jmm.0.026898-0

Cited by 49 publications

(23 citation statements)

References 10 publications

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“…The hydrophobic cells exhibit greater adherence to acrylic surfaces and host cells and tissues, and decreased susceptibility to being killed by polymorphonuclear neutrophils, which can contribute to the colonization and dissemination of the yeast [37]. Most Candida isolates exhibited hydrophobic property in this study, and as previously observed [38], this characteristic was strongly associated with biofilm formation on polystyrene surfaces. The formation of biofilm by Candida spp.…”

Section: Discussionsupporting

confidence: 75%

Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: antifungal susceptibility and virulence factors

Paula

Morey

Santos

et al. 2015

J Infect Dev Ctries

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Introduction: Host colonization by Candida species is an important predisposing factor to candidiasis, which seems to be more frequent in human immunodeficiency virus (HIV)-infected patients. Knowledge about the distribution, antifungal susceptibility, and virulence of oral Candida isolates is important for effective management of candidiasis. Methodology: Oral rinses were collected from 242 HIV-infected patients without clinical evidence of candidiasis seen at the AIDS referral center in Londrina, Brazil. Species were identified by standard phenotypic and molecular methods, and characterized in vitro according to antifungal susceptibility, cell surface hydrophobicity, biofilm formation, and enzyme activities. Results: Oral Candida colonization was detected in 50.4% of patients and combined use of antiretroviral therapy and protease inhibitor had a protective effect against colonization. Candida albicans (75.2%) was the most prevalent species. A high proportion of Candida spp. (39.9%) showed decreased susceptibility to fluconazole. Five isolates were resistant to nystatin. Protease and phospholipase activities were detected in 100% and 36.8% of isolates, respectively. Most isolates displayed a hydrophobic property that was associated with biofilm formation ability. Conclusions: A significant number of oral Candida species exhibiting decreased susceptibility to fluconazole were isolated from colonized HIV-infected individuals. Furthermore, all isolates expressed potential virulence attributes in vitro. Given the high incidence and severity of fungal infections in HIV-infected individuals, the results of this study reinforce the importance of antifungal susceptibility testing, which contributes to therapeutic strategies and highlights the need for continuous surveillance of Candida colonization in this population.

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Section: Discussionsupporting

confidence: 75%

Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: antifungal susceptibility and virulence factors

Paula

Morey

Santos

et al. 2015

J Infect Dev Ctries

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“…on different surfaces [32, 33]. Moreover, it has been shown that CSH of planktonic cells of C. albicans isolated from different sources correlates positively with biofilm formation on polystyrene [34]. In this study, the presence of eugenol (0.5 × MIC) caused a significant reduction in CSH and adhesion to polystyrene and HEp-2 cells of almost all planktonic cells of C. tropicalis and C. dubliniensis .…”

Section: Discussionmentioning

confidence: 63%

Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV‐Infected Patients

Paula

Bartelli

Raimo

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Most Candida spp. infections are associated with biofilm formation on host surfaces. Cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. The present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity of Candida dubliniensis and Candida tropicalis isolated from the oral cavity of HIV-infected patients. All isolates were able to form biofilms on different substrate surfaces. Eugenol showed inhibitory activity against planktonic and sessile cells of Candida spp. No metabolic activity in biofilm was detected after 24 h of treatment. Scanning electron microscopy demonstrated that eugenol drastically reduced the number of sessile cells on denture material surfaces. Most Candida species showed hydrophobic behavior and a significant difference in cell surface hydrophobicity was observed after exposure of planktonic cells to eugenol for 1 h. Eugenol also caused a significant reduction in adhesion of most Candida spp. to HEp-2 cells and to polystyrene. These findings corroborate the effectiveness of eugenol against Candida species other than C. albicans, reinforcing its potential as an antifungal applied to limit both the growth of planktonic cells and biofilm formation on different surfaces.

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“…demonstrated that the biomass or biofilms formed by some Candida spp. are associated with their hydrophobicity (Borghi et al ., ; Silva‐Dias et al ., ). Thus, we analysed biofilm formation by different T .…”

Section: Discussionmentioning

confidence: 99%

“…demonstrated that the biomass or biofilms formed by some Candida spp. are associated with their hydrophobicity (Borghi et al, 2011;Silva-Dias et al, 2015). Thus, we analysed biofilm formation by different T. asahii colony morphologies to investigate whether the cell surface hydrophobicity was a good predictor of biofilm formation in T. asahii.…”

Section: Discussionmentioning

confidence: 99%

Cell surface hydrophobicity and colony morphology ofTrichosporon asahiiclinical isolates

Ichikawa

Hirata

Takei

et al. 2016

Yeast

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Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity. The hydrophobicity of heat-killed T. asahii cells was greatly reduced after periodate oxidation of the cell surface carbohydrates. Furthermore, the cell wall and extracellular polysaccharide components differed among the morphologies. Our results suggest that T. asahii cell surface hydrophobicity is affected by cell surface carbohydrate composition. Copyright © 2016 John Wiley & Sons, Ltd.

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Cell surface hydrophobicity: a predictor of biofilm production in Candida isolates?

Cited by 49 publications

References 10 publications

Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: antifungal susceptibility and virulence factors

Oral Candida colonization in HIV-infected patients in Londrina-PR, Brazil: antifungal susceptibility and virulence factors

Effect of Eugenol on Cell Surface Hydrophobicity, Adhesion, and Biofilm of Candida tropicalis and Candida dubliniensis Isolated from Oral Cavity of HIV‐Infected Patients

Cell surface hydrophobicity and colony morphology ofTrichosporon asahiiclinical isolates

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