Cell surface hydrophobicity: a key component in the degradation of polyethylene succinate by<i>Pseudomonas</i>sp. AKS2

Tribedi, Prosun; Sil, Alok Kumar

doi:10.1111/jam.12375

Cited by 121 publications

(81 citation statements)

References 18 publications

(27 reference statements)

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“…The procedures of PA03 biofilm protein extraction were described previously with a few modifications [31]. Briefly, the preformed PA03 biofilms were incubated with or without AZM and BER alone and in combination at 1/2×MIC, 1×MIC and 2×MIC at 37°C for 24 h. After incubations, the biofilms were washed gently by sterile PBS to remove planktonic cells and adhered loosely cells.…”

Section: Methodsmentioning

confidence: 99%

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Synergistic Activity of Berberine with Azithromycin against Pseudomonas Aeruginosa Isolated from Patients with Cystic Fibrosis of Lung In Vitro and In Vivo

Huang

et al. 2017

Cell Physiol Biochem

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Background/Aims: Pseudomonas aeruginosa (PA) is one of the major opportunistic pathogens which can cause chronic lung infection of cystic fibrosis (CF). The formation of PA biofilm promotes CF development and restricts the antimicrobial efficacies of current antibiotics. Methods: The antimicrobial effects of azithromycin (AZM) and berberine (BER) alone and in combination were evaluated using microdilution method, checkerboard assay, time-kill test, qRT-PCR analysis and absorption method. The treatments of AZM and/or BER were further evaluated in an animal lung infection model via observing survival rate, bacterial burden and histopathology of lung, the levels of pro-/anti-inflammatory cytokines. Results: AZM-BER were demonstrated to be synergistic against ten clinical PA isolates as well as the standard reference PA ATCC27853, in which PA03 was the most susceptible isolate to AZM-BER with FICI of 0.13 and chosen for subsequent experiments. The synergism of AZM-BER was further confirmed against PA03 in time-kill test and scanning electron microscope (SEM) at their concentrations showing synergism. In PA03, we found that AZM-BER could significantly attenuate productions of a series of virulence factors including alginate, LasA protease, LasB protease, pyoverdin, pyocyanin, chitinase as well as extracellular DNA, and remarkably inhibit the levels of quorum sensing (QS) molecules and the expressions of lasI, lasR, rhlI, rhlR at 1/2×MIC, 1×MIC and 2×MIC. In the infection model, the mice survival were increased markedly, the inflammations of infected lungs were improved greatly along with reduced IL-6, IL-8 and ascended IL-10 at 0.8 mg/kg of AZM combined with 3.2 mg/kg of BER. Conclusion: BER might be a promising synergist to enhance the antimicrobial activity of AZM in vitro and in vivo.

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Section: Methodsmentioning

confidence: 99%

“…The alginates of PA03 biofilms were extracted and quantified as described previously with minor modifications [31]. Briefly, the preformed PA03 biofilms treated as above were scratched and washed by sterile PBS for several times.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Activity of Berberine with Azithromycin against Pseudomonas Aeruginosa Isolated from Patients with Cystic Fibrosis of Lung In Vitro and In Vivo

Huang

et al. 2017

Cell Physiol Biochem

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“…Pseudomonas sp. AKS2 cell surface hydrophobicity is important to the interaction between the cell and hydrophobic surface of polyethylene succinate (Liu et al 2012;Qiu et al 2003;Tribedi and Sil 2013b). Biostimulation (in situ bioremediation) has constructive effects on PESu degradation by establishing a new community and shows a founder effect (Tribedi and Sil 2013c).…”

Section: Polyethylene Succinatementioning

confidence: 99%

Review on the current status of polymer degradation: a microbial approach

Pathak

Bithel

2017

Bioresour. Bioprocess.

557

241

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Inertness and the indiscriminate use of synthetic polymers leading to increased land and water pollution are of great concern. Plastic is the most useful synthetic polymer, employed in wide range of applications viz. the packaging industries, agriculture, household practices, etc. Unpredicted use of synthetic polymers is leading towards the accumulation of increased solid waste in the natural environment. This affects the natural system and creates various environmental hazards. Plastics are seen as an environmental threat because they are difficult to degrade. This review describes the occurrence and distribution of microbes that are involved in the degradation of both natural and synthetic polymers. Much interest is generated by the degradation of existing plastics using microorganisms. It seems that biological agents and their metabolic enzymes can be exploited as a potent tool for polymer degradation. Bacterial and fungal species are the most abundant biological agents found in nature and have distinct degradation abilities for natural and synthetic polymers.

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“…Single factor test was designed in this study under different conditions of temperature (20,25,30,35, 40°C), pH (5,6,7,8,9) and initial concentrations of fenpropathrin (50, 100, 200, 400, 600, 800 mg l -1 ). Residual fenpropathrin concentration was determined at 24 h interval for 7 days.…”

Section: Degradation Experiments Of Fenpropathrinmentioning

confidence: 99%

“…The n-hexadecane was used for CSH determination of strain JQ-41. The concrete operation process of BATH had been described with some modifications [30]. The CSH value was calculated using Eq.…”

Section: Evaluation Of Bacterial Cell Surface Hydrophobicitymentioning

confidence: 99%

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

et al. 2015

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A soil bacterium designated strain JQ-41, capable of growth on fenpropathrin as the sole carbon source and energy source, was isolated from a long-term pyrethroid insecticide-treated orchard. Based on the morphology, physio-biochemical characteristics, and 16S rDNA gene analysis, as well as the G+C content of the genomic DNA, the strain JQ-41 was identified as Pseudomonas aeruginosa. Up to 92.3% of 50 mg l(-1) fenpropathrin was degraded by P. aeruginosa strain at 30°C and pH 7 within 7 days. The kinetic parameters q max, K s, and K i were established to be 1.14 day(-1), 38.41 mg l(-1), and 137.67 mg l(-1), respectively, and the critical inhibitor concentration was determined to be 72.72 mg l(-1). Cell surface hydrophobicity of P. aeruginosa strain was enhanced during growth on fenpropathrin. Three metabolites from fenpropathrin degradation were identified by gas chromatography mass spectrometry, and then a possible degradation pathway was proposed. In addition, this isolate was also able to degrade a wide range of synthetic pyrethroid insecticides including cypermethrin, deltamethrin, bifenthrin, and cyhalothrin with the degradation process following the first-order kinetic model. Taken together, our results provide insights into the kinetics and mechanism of fenpropathrin degradation by P. aeruginosa strain and also highlight its promising potential in bioremediation of pyrethroid-contaminated environment.

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Cell surface hydrophobicity: a key component in the degradation of polyethylene succinate byPseudomonassp. AKS2

Cited by 121 publications

References 18 publications

Synergistic Activity of Berberine with Azithromycin against Pseudomonas Aeruginosa Isolated from Patients with Cystic Fibrosis of Lung In Vitro and In Vivo

Synergistic Activity of Berberine with Azithromycin against Pseudomonas Aeruginosa Isolated from Patients with Cystic Fibrosis of Lung In Vitro and In Vivo

Review on the current status of polymer degradation: a microbial approach

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

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