Cell surface chondroitin sulfate proteoglycans in tumor cell adhesion, motility and invasion

Iida, Joji; Meijne, A. M. L.; Knutson, Jennifer R.; Furcht, Leo T.; McCarthy, James B.

doi:10.1006/scbi.1996.0021

Cited by 90 publications

(59 citation statements)

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“…The available evidence suggests that, in addition to being a useful target for immunotherapy of melanoma, HMW-MAA plays a critical role in tumor growth and metastasis (Mittelman et al, 1992;Iida et al, 1996;Burg et al, 1998). Despite the biological importance of HMW-MAA, to date there has been no studies of the are closely linked, suggesting that a transcriptional mechanism regulates HMW-MAA expression.…”

Section: Discussionmentioning

confidence: 99%

“…Several lines of evidence suggest that HMW-MAA, like the rat ortholog NG2, plays important roles in intracellular signal cascades important for cellular adhesion, spreading and invasion (Burg et al, 1998;Chekenya et al, 1999;Fang et al, 1999;Iida et al, 1996Iida et al, , 2001. These include the activation of small Rho family GTPase Cdc42 and of the adaptor protein p130cas (Eisenmann et al, 1999), as well as the association of HMW-MAA with membrane-type 3 matrix metalloproteinase on melanoma cells (Iida et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells

et al. 2006

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The human high molecular weight-melanoma associated antigen (HMW-MAA) is a membrane-bound chondroitin sulfate proteoglycan that is variably expressed in a high percentage of melanoma cell lines and tumors. Since the mechanism(s) regulating HMW-MAA expression has(ve) not been defined, in this study, we have examined whether promoter DNA methylation regulates the level of HMW-MAA expression. In melanoma cell lines, the level of HMW-MAA mRNA and protein expression is coordinately regulated, implicating a transcriptional control mechanism. Consistent with a role for regulation by DNA methylation, we have found that a dense CpG island flanks the human HMW-MAA gene transcriptional start site. Methylation-specific PCR and sodium bisulfite DNA sequencing analyses indicate that the HMW-MAA promoter is heavily methylated in melanoma cell lines, melanoma lesions and normal lymphocytes that do not express HMW-MAA; in contrast, the HMW-MAA promoter is not methylated in melanoma cell lines and tumors that express this antigen. In addition, HMW-MAA expression is markedly induced in HMW-MAAnegative melanoma cell lines by incubation with the DNA methyltransferase inhibitor 5-aza-2 0 -deoxycytidine. In summary, our results establish DNA methylation as a key regulator of HMW-MAA expression by human melanoma cells. This information represents a useful background to optimize immunotherapeutic strategies targeting HMW-MAA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells

et al. 2006

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“…Increasing evidence suggests that CS-PGs are also related to metastatic potential in addition to HS-PGs (10 -12). CS-PGs at the tumor cell surface and in the extracellular matrix facilitate tumor invasion by enhancing integrin-mediated cell adhesion, motility, and intracellular signaling (12). The expression of versican is up-regulated in many tumors including lung cancer, as a macrophage activator that acts through Toll-like receptor-2 (TLR2) and its co-receptors TLR6 and CD14 (13).…”

mentioning

confidence: 99%

Receptor for Advanced Glycation End Products (RAGE) Functions as Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or Sulfated Glycosaminoglycans Delivered in Vivo Inhibit Pulmonary Metastasis of Tumor Cells

Mizumoto¹,

Takahashi²,

Sugahara

2012

Journal of Biological Chemistry

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“…Melanoma cells have been shown to possess at least two distinct cell surface chondroitin sulfate proteoglycans, CD44 and the melanoma-associated proteoglycan/melanoma chondroitin sulfate proteoglycan (4,28). CD44 binds directly to type IV collagen, and this binding is dependent upon chondroitin sulfate (CS) (21).…”

mentioning

confidence: 99%

Differential Modulation of Human Melanoma Cell Metalloproteinase Expression by α2β1 Integrin and CD44 Triple-helical Ligands Derived from Type IV Collagen

Baronas-Lowell

Lauer‐Fields

Borgia³

et al. 2004

Journal of Biological Chemistry

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Tumor cell binding to components of the basement membrane is well known to trigger intracellular signaling pathways. Signaling ultimately results in the modulation of gene expression, facilitating metastasis. Type IV collagen is the major structural component of the basement membrane and is known to be a polyvalent ligand, possessing sequences bound by the ␣ 1 ␤ 1 , ␣ 2 ␤ 1 , and ␣ 3 ␤ 1 integrins, as well as cell surface proteoglycan receptors, such as CD44/chondroitin sulfate proteoglycan (CSPG). The role of ␣ 2 ␤ 1 integrin and CD44/CSPG receptor binding on human melanoma cell activation has been evaluated herein using triple-helical peptide ligands incorporating the ␣1(IV)382-393 and ␣1(IV)1263-1277 sequences, respectively. Gene expression and protein production of matrix metalloproteinases-1 (MMP-1), -2, -3, -13, and -14 were modulated with the ␣ 2 ␤ 1 -specific sequence, whereas the CD44-specific sequence yielded significant stimulation of MMP-8 and lower levels of modulation of MMP-1, -2, -13, and -14. Analysis of enzyme activity confirmed different melanoma cell proteolytic potentials based on engagement of either the ␣ 2 ␤ 1 integrin or CD44/CSPG. These results are indicative of specific activation events that tumor cells undergo upon binding to select regions of basement membrane collagen. Based on the present study, triple-helical peptide ligands provide a general approach for monitoring the regulation of proteolysis in cellular systems.Melanoma is one of the most rapidly increasing malignancies in the world in both young and old patients, with over 50,000 newly diagnosed patients each year (1). 1 Mortality from cancer is frequently due to metastasis, given that surgical excision of the primary tumor considerably enhances the prognosis of a patient and prolongs survival (2). Metastasis is a complex series of finely coordinated events that results in cancer cells circulating in lymph and blood vascular systems to invade remote tissues and establish secondary sites of tumor growth (3). Extravasation of the tumor cell into secondary tissues requires alterations in cellular behaviors, resulting from specific adhesion to components of the basement membrane. Tumor cells respond to each of the various components of the ECM, 2 including the collagens; noncollagenous glycoproteins, such as fibronectin and laminin; and proteoglycans, such as decorin and syndecan (4). These interactions are known to occur through several families of cell surface receptors, including the integrins and cell surface proteoglycans.Integrins are heterodimeric proteins composed of one ␣ and one ␤ subunit and are the best described of the cell surface adhesion molecules. To date, there are 18 different ␣ subunits and 8 distinct ␤ subunits identified that combine to form at least 24 heterodimers (5-7). Although the specific integrin expression profile can fluctuate with tumor type and stage of progression, highly metastatic melanoma cells are known to up-regulate expression of, and ␣ 6 ␤ 4 integrins while down-regulating the expression ...

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Cell surface chondroitin sulfate proteoglycans in tumor cell adhesion, motility and invasion

Cited by 90 publications

References 0 publications

Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells

Regulation of high molecular weight-melanoma associated antigen (HMW-MAA) gene expression by promoter DNA methylation in human melanoma cells

Receptor for Advanced Glycation End Products (RAGE) Functions as Receptor for Specific Sulfated Glycosaminoglycans, and Anti-RAGE Antibody or Sulfated Glycosaminoglycans Delivered in Vivo Inhibit Pulmonary Metastasis of Tumor Cells

Differential Modulation of Human Melanoma Cell Metalloproteinase Expression by α2β1 Integrin and CD44 Triple-helical Ligands Derived from Type IV Collagen

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