Cell surface charge, sialic acid content and metabolic behaviour of two tumour sublines. A comparative study

Bohn, B.; Thies, Cameron G.; Brossmer, Reinhard

doi:10.1016/0014-2964(77)90013-5

Cited by 6 publications

(1 citation statement)

References 22 publications

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“…The Ehrlich ascites tumor cell has a negative zeta potential as determined by cell electrophoresis and the cell membrane contains negative charges related in part to the carboxyl group of sialic acid [4,51]. Accordingly, cells were incubated with or without 10 units of neuraminidase per ml as described in Materials and Methods for 30 min and washed twice in saline.…”

Section: Relationship Between Cell Surface Charge and The Effects Of mentioning

confidence: 99%

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Fry

Goldman

1982

J. Membrain Biol.

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Interaction of positively (phosphatidylcholine/stearylamine 5:1) or negatively (phosphatidylcholine/stearic acid 5:1) charged liposomes with Ehrlich ascites tumor cells for 1-5 min increases or decreases, respectively, the bidirectional fluxes of the folic acid analog, methotrexate. These effects on influx and efflux appear to be symmetrical since the liposomes do not change the intracellular level of methotrexate at the steady state. Influx kinetics show that these alterations result from an increase or decrease in the Vmax with no change in the Kinm. These effects appear to be specific for the methotrexate-tetrahydrofolate carrier system since the transport of other compounds which utilize this carrier, aminopterin, 5-methyltetrahydrofolate, and 5-formyltetrahydrofolate, is affected similarly to methotrexate, whereas, the transport of folic acid, a compound similar in structure and charge but not significantly transported by this carrier is unaffected by liposomes. Once cells are exposed to charged liposomes, the effects on methotrexate transport cannot be reversed by washing the cells free of the extracellular liposomes. If, however, cells are exposed to liposomes of one charge, washed and then exposed to liposomes of the opposite charge, methotrexate influx is reversed to control rates. The effects of charged liposomes on methotrexate influx were not abolished by treating the cells with neuraminidase, metabolic inhibitors or lowering the temperature to 4 degrees C. Studies on the uptake of [14C] liposomes show that these effects are not proportional to the total amount of lipid associated with the cell but result from an initial rapid liposome-cell association that is not dependent on temperature or energy metabolism nor related to cell surface charge.

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Section: Relationship Between Cell Surface Charge and The Effects Of mentioning

confidence: 99%

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Fry

Goldman

1982

J. Membrain Biol.

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Difference in plasma membrane structure between two sublines of ehrlich-lettrè ascites tumor cells

Haeffner

Heck

Kolbe

1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Modification of tumour cells by covalent attachment of N-acetyl-d-neuraminic acid to the cell surface

Brossmer¹,

Bohn²,

Thies³

1977

European Journal of Cancer (1965)

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Cell surface charge, sialic acid content and metabolic behaviour of two tumour sublines. A comparative study

Cited by 6 publications

References 22 publications

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Further studies on the charge-related alterations of methotrexate transport in Ehrlich ascites tumor cells by ionic liposomes: Correlation with liposome-cell association

Difference in plasma membrane structure between two sublines of ehrlich-lettrè ascites tumor cells

Modification of tumour cells by covalent attachment of N-acetyl-d-neuraminic acid to the cell surface

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