Cell surface antigens of murine leukemias induced by radiation leukemia virus. Recognition of individually distinct cell surface antigens by cytotoxic T cells on leukemias expressing crossreactive transplantation antigens.

Abstract

Antigens specific for individual tumors were first discovered in experiments that showed the rejection of chemically induced sarcomas in mice and rats that had been immunized with the same tumor (1). Subsequently, individually distinct antigens were also found in transplantation studies of murine reticulum cell sarcomas and mammary tumors. Detailed study of these unique antigens has long been hampered by the considerable difficulties encountered in attempts to detect them in vitro. Success was first reported in a study of the methylcholanthrene-induced BALB/c sarcoma Meth A. Hyperimmunization ofsyngeneic mice with this tumor resulted in the production of antibodies recognizing a cell surface antigen whose expression showed the same restriction as the antigen detected in transplantation experiments (2). More recent serological studies of feline leukemias, and of murine sarcomas induced by the Rous sarcoma virus, have also defined individually distinct cell surface antigens expressed only on the tumor used for immunization. With the advances in the serological analysis of human tumors brought about by the development of autologous typing, unique cell surface antigens have also been found on human cancers--melanoma, astrocytoma, and renal cancer (2).Although individually distinct tumor antigens were initially shown in transplantation experiments generally considered to reflect cellular immunity, there have only been a few reports describing recognition of unique tumor antigens by cytotoxic T cells in vitro (3, 4). We report here that leukemias induced in mice by the radiation leukemia virus (RadLV) express individually distinct antigens recognized by cytotoxic T cells, in addition to crossreacting tumorspecific antigens detected in transplantation experiments.

Induction of in vitro and in vivo anti‐tumor responses by sensitization of mice with liposomes containing a crude butanol extract of leukemia cells and transferred inter‐membranously with cell‐surface proteins

“…6 , but not 5 other RadLV-induced leukemias of BALBIc origin, 3 Ra-dLV-induced leukemias of B6 origin, a radiation leukemia of BALBJc origin, a chemically induced leukemia of B6 origin, or normal spleen Con A blasts. Morishita et al (1986) have reported that when the BALBRVD tumor was inoculated into female CB6Fl mice, CTL reactive against only BALBRVD were generated in spleen cells from regressor mice following in vitro sensitization. However, the CTL similarly generated in this study were cross-reactive (Table 111).…”

“…Tumors RVE, BALBRV1, BALBRV2 and BALBRV3 are leukemias induced by injection of radiation leukemia virus (RadLV) into neonatal BALB/c mice (Nakayama et al, 1984(Nakayama et al, , 1986Morishita et al, 1986). B6RV1, B6RV2 and B6RV4 are leukemias similarly induced in B6 mice (Nakayama et al, 1984(Nakayama et al, , 1986.…”

“…Tumor-rejection antigens were first found on methylcholanthrene-induced sarcomas in mice (Gross, 1943;Foley, 1953;Prehn and Main, 1957) and later on a variety of other tumors. Tumor-rejection antigens on leukemias appear to be associated with murine leukemia virus (MuLV) (Sato et al, 1973;Nakayama et al, 1984Nakayama et al, , 1986Morishita et al, 1986). Analysis of cellular immune responses in syngeneic or semi-syngeneic mice revealed that CD8 + cytotoxic T lymphocytes (CTL) were efficiently generated in vitro against leukemias on which tumor-rejection antigens had been demonstrated (Nakayama et al, 1979(Nakayama et al, , 1984Morishita et al, 1986).…”

Therapeutic use of a long-term cytotoxic T cell line recognizing a common tumour-associated antigen: The pattern of in vitro reactivity predicts the in vivo effect on different tumours

“…Common TAA have been detected by transplantation studies and by specific cytotoxic T lymphocytes (CTL) in mouse melanomas [6], chemically induced sarcomas [7][8][9], radiation-induced leukemias [ 10] and colonic carci-54 nomas [14,20]. Common TAA of human tumours, as defined by T cell clones that specifically lyse the autologous tumour as well as allogeneic tumours that express the same restricting human leucocyte antigen A, have been found among melanomas and soff tissue and bone sarcomas [4,22].…”