Cell supports of chitosan/hyaluronic acid and chondroitin sulphate systems. Morphology and biological behaviour

Abstract

Films and sponges of chitosan (CHI), chitosan/hyaluronic acid (CHI-HA) and chitosan/chondroitin sulphate (CHI-CHOS), were prepared by film deposition or lyophilization (sponges), avoiding the formation of interpolyelectrolyte complexes. The biological behaviour of the systems was analysed by studying the cell behaviour using a fibroblast cell line and standard biological MTT and Alamar Blue tests. The morphology of films, sponges and cell seeded samples was analysed by ESEM. The results obtained indicate that all the systems can be considered as good supports for cell adhesion and proliferation, but there is specific activation of the proliferative process in the presence of hyaluronic acid and chondroitin sulphate.

Poly(N‐isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three‐dimensional stem cell culture and cartilage tissue engineering

“…However, poor cell adhesion on chitosan scaffolds has been previously reported . Functionalization of chitosan by incorporating collagen type I or II, hyaluronic acid, and other molecules is the widely accepted means to improve the cell seeding and adhension . However, it is very challenging to obtain uniform cell seeding into the scaffold.…”

Untitled

“…In addition, the use of hydrophilic bases within the required range allows regulating the biopharmaceutical and structural and mechanical properties of dental gels. [27][28][29][30][31][32] Cellulose derivatives are widely used as hydrophilic bases for dental products: Methylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, and others. Gels formed by these compounding are compatible with many medicinal substances, do not cause irritation, can form homogeneous mixtures with the secrets of the mucous membranes, have adsorption properties absorbing exudates.…”

Chitosan Derivatives

“…The surface chemical composition of PECs is important in cell events because the cell could directly recognize the PECs and adheres to them without the aid of protein. A higher adhesion number of cells on the chitosan/chondroitin sulfate surface are better than that on pure chitosan films [17]. CS-PECs containing carboxymethyl groups as anionic sites cause the human periodontal ligament fibroblast (HPLF) to aggregate and promote differentiation because the carboxymethyl groups offer similar conditions as in vivo to HPLF.…”