Cell-substratum and cell-cell adhesion forces and single-cell mechanical properties in mono- and multilayer assemblies from robotic fluidic force microscopy

Nagy, A.; Székács, Inna; Bonyár, Attila; Horváth, Róbert

doi:10.1016/j.ejcb.2022.151273

Cited by 10 publications

(8 citation statements)

References 70 publications

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“…Consistent with conventional FluidFM-based SCFS data, 23–25,47 all conditions exhibited a positive linear regression and correlation between F adh – D max , W adh – D max , and W adh – F adh (Fig. S2a–c†); higher forces of adhesion required greater detachment distances and larger detachment energies.…”

Section: Resultssupporting

confidence: 83%

“…From a biological perspective, the resultant average S c suggests that cells grown on nano-roughened surfaces have smaller deformation capabilities when being pulled from the surface, and thus are stiffer compared to cells grown on smoother surfaces. However, cells with shorter D max are typically stiffer than cells retaining larger D max , 25 and accordingly, SH-SY5Y cells cultured on roughened PI surfaces appear to be more elastic because they have higher D max values compared to cells grown on smooth surfaces (Fig. 4c).…”

Section: Single-cell Force Measurements Of Sh-sy5y Neural Cells Cultu...mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

“…21,22 FluidFM-based SCFS has been used to assess adhesion forces of mammalian cell lines to underlying substrates enabling label-free, rapid, and reliable adhesion detachment recordings while providing a high signal-tonoise ratio without altering cell physiological properties. [23][24][25][26][27][28][29][30] In this work, we measure the impact of nanoscale surface roughness on single-cell neural adhesion and mechanical coupling to underlying substrates. We optimized plasma treatment to produce a reproducible process capable of tailoring precise polyimide surface roughness with no chemical modification.…”

Section: Introductionmentioning

confidence: 99%

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Single-cell fluid-based force spectroscopy reveals near lipid size nano-topography effects on neural cell adhesion

Habli,

Lahoud,

Zantout

et al. 2024

Lab Chip

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show abstract

Section: Resultssupporting

confidence: 83%

Section: Single-cell Force Measurements Of Sh-sy5y Neural Cells Cultu...mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Single-cell fluid-based force spectroscopy reveals near lipid size nano-topography effects on neural cell adhesion

Habli,

Lahoud,

Zantout

et al. 2024

Lab Chip

View full text Add to dashboard Cite

show abstract

“…In spite of these highly sophisticated methods, a very robust method to position cells for research purposes is to use a cantilever system with integrated microfluidics, which allows for picking up cells and releasing them at desired positions. [195] Also extrusion printing methods develop into systems to build more complex tissues and more precise deposition of different cell types. This can be achieved, e.g., in suspension as demonstrated by Daly et al by printing and positioning spheroids into hydrogels that then join into the desired tissue shape, [196] or by the use of microreactor structures as shown by Cho et al for a joint system of blood and lymph vessels for modelling more realistic setups in targeted cancer therapy.…”

Section: Engineering the Spatial Contact Of Cells With Bioelectronic ...mentioning

confidence: 99%

Printed Electronic Devices and Systems for Interfacing with Single Cells up to Organoids

Saghafi,

Vasantham,

Hussain

et al. 2023

Adv Funct Materials

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The field of bioelectronics with the aim to contact cells, cell clusters, biological tissues and organoids has become a vast enterprise. Currently, it is mainly relying on classical micro‐ and nanofabrication methods to build devices and systems. Very recently the field is highly pushed by the development of novel printable organic, inorganic and biomaterials as well as advanced digital printing technologies such as laser and inkjet printing employed in this endeavor. Recent advantages in alternative additive manufacturing and 3D printing methods enable interesting new routes, in particular for applications requiring the incorporation of delicate biomaterials or creation of 3D scaffold structures that show a high potential for bioelectronics and building of hybrid bio‐/inorganic devices. Here the current state of printed 2D and 3D electronic structures and related lithography techniques for the interfacing of electronic devices with biological systems are reviewed. The focus lies on in vitro applications for interfacing single cell, cell clusters, and organoids. Challenges and future prospects are discussed for all‐printed hybrid bio/electronic systems targeting biomedical research, diagnostics, and health monitoring.

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Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment

Li,

Sun,

Hou

et al. 2023

Small

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Understanding the signals from the physical microenvironment is critical for deciphering the processes of neurogenesis and neurodevelopment. The discovery of how surrounding physical signals shape human developing neurons is hindered by the bottleneck of conventional cell culture and animal models. Notwithstanding neural organoids provide a promising platform for recapitulating human neurogenesis and neurodevelopment, building neuronal physical microenvironment that accurately mimics the native neurophysical features is largely ignored in current organoid technologies. Here, it is discussed how the physical microenvironment modulates critical events during the periods of neurogenesis and neurodevelopment, such as neural stem cell fates, neural tube closure, neuronal migration, axonal guidance, optic cup formation, and cortical folding. Although animal models are widely used to investigate the impacts of physical factors on neurodevelopment and neuropathy, the important roles of human stem cell‐derived neural organoids in this field are particularly highlighted. Considering the great promise of human organoids, building neural organoid microenvironments with mechanical forces, electrophysiological microsystems, and light manipulation will help to fully understand the physical cues in neurodevelopmental processes. Neural organoids combined with cutting‐edge techniques, such as advanced atomic force microscopes, microrobots, and structural color biomaterials might promote the development of neural organoid‐based research and neuroscience.

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Cell-substratum and cell-cell adhesion forces and single-cell mechanical properties in mono- and multilayer assemblies from robotic fluidic force microscopy

Cited by 10 publications

References 70 publications

Single-cell fluid-based force spectroscopy reveals near lipid size nano-topography effects on neural cell adhesion

Single-cell fluid-based force spectroscopy reveals near lipid size nano-topography effects on neural cell adhesion

Printed Electronic Devices and Systems for Interfacing with Single Cells up to Organoids

Engineering the Physical Microenvironment into Neural Organoids for Neurogenesis and Neurodevelopment

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