Cell structure and microtubule organisation during gametogenesis of Ulva mutabilis Føyn (Chlorophyta)

Katsaros, Christos; Weiss, Anne; Llangos, Ira; Théodorou, Ioannis; Wichard, Thomas

doi:10.1515/bot-2016-0073

Cited by 17 publications

(6 citation statements)

References 36 publications

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“…The microtubule cytoskeleton of somatic cells consists of parallel microtubule bundles arranged mainly in the cortical cytoplasm parallel to the plasmalemma. However, 36 h after induction, the microtubule bundles traverse the cortical cytoplasm converging on a particular area and then the microtubules starting from a pointed site at basal part of gametangium and forming a basket-like con guration with a circular opening at the top after 48 h [27]. Stratmann et al (1996) analyzed the DNA synthesis during gametogenesis and concluded that DNA replication starts from 25 hours after induction which is consistent with our RNA-seq data.…”

Section: Discussionsupporting

confidence: 84%

Transcriptional profiling of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP‐RK transcription factors linked to reproduction

Liu

Blomme

Bogaert

et al. 2021

Preprint

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Background The molecular mechanism underlying sexual reproduction in land plants is well understood in model plants and is a target for crop improvement. However, unlike land plants, the genetic basis involved in triggering reproduction and gamete formation remains elusive in most seaweeds, which are increasingly viewed as an alternative source of functional food and feedstock for energy applications. Results Gametogenesis of Ulva mutabilis, a model organism for green seaweeds, is studied. We analyze transcriptome dynamics at different time points during gametogenesis following induction of reproduction by fragmentation and removal of sporulation inhibitors. Analyses demonstrate that 45% of the genes in the genome are differentially expressed during gametogenesis. We identified several transcription factors that potentially play a key role in the early gametogenesis of Ulva given the function of their homologs in higher plants and microalgae. In particular, the detailed expression pattern of an evolutionary conserved transcription factor containing an RWP-RK domain suggests a key role during Ulva gametogenesis. Conclusions Transcriptomic analyses of gametogenesis in the green seaweed Ulva highlight the importance of a conserved RWP-RK transcription factor in induction of sexual reproduction. The identification of putative master regulators of gametogenesis provides a starting point for further functional characterization.

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Section: Discussionsupporting

confidence: 84%

Transcriptional profiling of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP‐RK transcription factors linked to reproduction

Liu

Blomme

Bogaert

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, 36 h after induction, the microtubule bundles traverse the cortical cytoplasm converging on a particular area. Then, the microtubules start from a pointed site at the basal part of gametangium and form a basketlike configuration with a circular opening at the top after 48 h [27]. Stratmann et al [15] analyzed DNA synthesis during gametogenesis and concluded that DNA replication starts from 25 h after induction, which is consistent with our RNA-seq data.…”

Section: Dynamic Changes In Gene Regulation During Gametogenesissupporting

confidence: 86%

“…Genes related to DNA replication and microtubulebased movement were significantly upregulated after this irreversible timepoint. Electron microscope observations indeed demonstrate drastic rearrangements in microtubule morphology between 36 h and 48 h after induction [27]. The microtubule cytoskeleton of somatic cells consists of parallel microtubule bundles arranged mainly in the cortical cytoplasm parallel to the plasmalemma.…”

Section: Dynamic Changes In Gene Regulation During Gametogenesismentioning

confidence: 85%

Transcriptional dynamics of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP-RK transcription factor linked to reproduction

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background The molecular mechanism underlying sexual reproduction in land plants is well understood in model plants and is a target for crop improvement. However, unlike land plants, the genetic basis involved in triggering reproduction and gamete formation remains elusive in most seaweeds, which are increasingly viewed as an alternative source of functional food and feedstock for energy applications. Results Gametogenesis of Ulva mutabilis, a model organism for green seaweeds, was studied. We analyzed transcriptome dynamics at different time points during gametogenesis following induction of reproduction by fragmentation and removal of sporulation inhibitors. Analyses demonstrated that 45% of the genes in the genome were differentially expressed during gametogenesis. We identified several transcription factors that potentially play a key role in the early gametogenesis of Ulva given the function of their homologs in higher plants and microalgae. In particular, the detailed expression pattern of an evolutionarily conserved transcription factor containing an RWP-RK domain suggested a key role during Ulva gametogenesis. Conclusions Transcriptomic analyses of gametogenesis in the green seaweed Ulva highlight the importance of a conserved RWP-RK transcription factor in the induction of sexual reproduction. The identification of putative master regulators of gametogenesis provides a starting point for further functional characterization.

show abstract

“…The microtubule cytoskeleton of somatic cells consists of parallel microtubule bundles arranged mainly in the cortical cytoplasm parallel to the plasmalemma. However, 36 h after induction, the microtubule bundles traverse the cortical cytoplasm converging on a particular area and then the microtubules starting from a pointed site at basal part of gametangium and forming a basket-like configuration with a circular opening at the top after 48 h (Katsaros et al , 2017). Stratmann et al (1996) analyzed the DNA synthesis during gametogenesis and concluded that DNA replication starts from 25 hours after induction which is consistent with our RNA-seq data.…”

Section: Discussionmentioning

confidence: 99%

Gametogenesis in the green seaweed Ulva mutabilis coincides with massive transcriptional restructuring

Liu

Blomme

Bogaert

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The molecular mechanism underlying sexual reproduction in land plants is well understood in model plants and is a target for crop improvement. However, unlike land plants, the genetic basis involved in triggering reproduction and gamete formation remains elusive in most seaweeds, which are increasingly viewed as an alternative source of functional food and feedstock for energy applications. Here, gametogenesis of Ulva mutabilis, a model organism for green seaweeds, is studied. We analyze transcriptome dynamics at different time points during gametogenesis following induction of reproduction by fragmentation and removal of sporulation inhibitors. Analyses demonstrate that 45% of the genes in the genome are differentially expressed during gametogenesis. We identified several transcription factors that potentially play a key role in the early gametogenesis of Ulva given the function of their homologs in higher plants and microalgae. In particular, the detailed expression pattern of an evolutionary conserved transcription factor containing an RWP-RK domain suggests a key role during Ulva gametogenesis. The identification of putative master regulators of gametogenesis provides a starting point for further functional characterization.

show abstract

Cell structure and microtubule organisation during gametogenesis of Ulva mutabilis Føyn (Chlorophyta)

Cited by 17 publications

References 36 publications

Transcriptional profiling of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP‐RK transcription factors linked to reproduction

Transcriptional profiling of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP‐RK transcription factors linked to reproduction

Transcriptional dynamics of gametogenesis in the green seaweed Ulva mutabilis identifies an RWP-RK transcription factor linked to reproduction

Gametogenesis in the green seaweed Ulva mutabilis coincides with massive transcriptional restructuring

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