Cell stress response to two different types of polymer coated cobalt ferrite nanoparticles

Lojk, Jasna; Strojan, Klemen; Miš, Katarina; Bregar, Boštjan Vladimir; Bratkovič, Iva Hafner; Bizjak, Maruša; Pirkmajer, Sergej; Pavlin, Mojca

doi:10.1016/j.toxlet.2017.02.010

Cited by 14 publications

(7 citation statements)

References 57 publications

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“…Generally, PEI or PEI-based particles can stimulate the immune system, accompanied by the induction of various sorts of cell stress and immune response-related transcription factors. 117 Yet, it is notoriously difficult to elucidate the mechanisms clearly. The evidence for adjuvant effect of PEI or PEI-based particles in different research is generalized and drawn out by our own understanding (Figure 8).…”

Section: Summary Of the Immunoactivation Mechanisms Of Peimentioning

confidence: 99%

“…25,97 Besides, ROS can be induced by the primary generation, or the product from cell stress, while NF-κB is the key suppressor of ROS-induced apoptosis. 117 Other intermediates (such as the dectin-1) intermediate bioactive cytokines and chemokines (such as the CCL family), and accessory immune cells (such as neutrophils and invariant NK T cells) are also involved in the network. 119…”

Section: Summary Of the Immunoactivation Mechanisms Of Peimentioning

confidence: 99%

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Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Shen¹,

Li²,

Zhang³

et al. 2017

IJN

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Vaccines have shown great success in treating and preventing tumors and infections, while adjuvants are always demanded to ensure potent immune responses. Polyethylenimine (PEI), as one of the well-studied cationic polymers, has been used as a transfection reagent for decades. However, increasing evidence has shown that PEI-based particles are also capable of acting as adjuvants. In this paper, we briefly review the physicochemical properties and the broad applications of PEI in different fields, and elaborate on the intracellular processes of PEI-based vaccines. In addition, we sum up the proof of their in vivo and clinical applications. We also highlight some mechanisms proposed for the intrinsic immunoactivation function of PEI, followed by the challenges and future perspectives of the applications of PEI in the vaccines, as well as some strategies to elicit the desirable immune responses.

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Section: Summary Of the Immunoactivation Mechanisms Of Peimentioning

confidence: 99%

Section: Summary Of the Immunoactivation Mechanisms Of Peimentioning

confidence: 99%

Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Shen¹,

Li²,

Zhang³

et al. 2017

IJN

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“…A continuous exposure simulated events of repeated or continuous exposure to NPs due to the presence of NPs in different consumer products or environmental pollution. We used four types of NPs: two biomedically relevant (magnetic PAA and PEI) NPs [ 8 , 56 , 57 , 58 , 59 ] and industrially relevant (SiO 2 and TiO 2 ) NPs as representative types of two very commonly used commercial NPs in various consumer products and materials. We analyzed the effects of NPs on the cell viability, morphology, and ultrastructure on both cell models.…”

Section: Discussionmentioning

confidence: 99%

“…NPs can induce lower proliferation rates, oxidative stress (ROS; reactive oxygen species formation), changes in cell metabolism and cell signaling, DNA damage, autophagy and lysosomal dysfunctions, and changes in the morphology, and they can also disrupt the cell cytoskeleton and hinder cell differentiation [ 5 , 6 , 7 ], depending on their properties, concentration, and exposure time. NPs can also trigger pro-inflammatory reactions through several mechanisms, including nuclear factor κB (NF-κB) activation, inflammasome activation, or others [ 8 , 9 ]. The toxicity of NPs can also be a result of ion leakage or a consequence of released toxic degradation products [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

Skočaj

Bizjak

Strojan

et al. 2020

IJMS

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Many studies evaluated the short-term in vitro toxicity of nanoparticles (NPs); however, long-term effects are still not adequately understood. Here, we investigated the potential toxic effects of biomedical (polyacrylic acid and polyethylenimine coated magnetic NPs) and two industrial (SiO2 and TiO2) NPs following different short-term and long-term exposure protocols on two physiologically different in vitro models that are able to differentiate: L6 rat skeletal muscle cell line and biomimetic normal porcine urothelial (NPU) cells. We show that L6 cells are more sensitive to NP exposure then NPU cells. Transmission electron microscopy revealed an uptake of NPs into L6 cells but not NPU cells. In L6 cells, we obtained a dose-dependent reduction in cell viability and increased reactive oxygen species (ROS) formation after 24 h. Following continuous exposure, more stable TiO2 and polyacrylic acid (PAA) NPs increased levels of nuclear factor Nrf2 mRNA, suggesting an oxidative damage-associated response. Furthermore, internalized magnetic PAA and TiO2 NPs hindered the differentiation of L6 cells. We propose the use of L6 skeletal muscle cells and NPU cells as a novel approach for assessment of the potential long-term toxicity of relevant NPs that are found in the blood and/or can be secreted into the urine.

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“…Interaction of NPs with mammalian cells depends on the size as well as the chemical and physical properties of the respective particle . Surface characteristics of NPs, like surface coating, are also decisive for toxicity . Previous studies have demonstrated that NPs can cause cell damage directly by passing through the cell membrane, or indirectly by inducing reactive oxygen species (ROS) or inflammation .…”

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confidence: 99%

Next‐Generation Magnetic Nanocomposites: Cytotoxic and Genotoxic Effects of Coated and Uncoated Ferric Cobalt Boron (FeCoB) Nanoparticles In Vitro

Netzer

Jordakieva

Girard

et al. 2017

Basic Clin Pharma Tox

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Metal nanoparticles (NPs) have unique physicochemical properties and a widespread application scope depending on their composition and surface characteristics. Potential biomedical applications and the growing diversity of novel nanocomposites highlight the need for toxicological hazard assessment of next-generation magnetic nanomaterials. Our study aimed to evaluate the cytotoxic and genotoxic properties of coated and uncoated ferric cobalt boron (FeCoB) NPs (5-15 nm particle size) in cultured normal human dermal fibroblasts. Cell proliferation was assessed via ATP bioluminescence kit, and DNA breakage and chromosomal damage were measured by alkaline comet assay and micronucleus test. Polyacryl acid-coated FeCoB NPs [polyacrylic acid (PAA)-FeCoB NPs) and uncoated FeCoB NPs inhibited cell proliferation at 10 μg/ml. DNA strand breaks were significantly increased by PAA-coated FeCoB NPs, uncoated FeCoB NPs and l-cysteine-coated FeCoB NPs (Cys-FeCoB NPs), although high concentrations (10 μg/ml) of coated NPs (Cys- and PAA-FeCoB NPs) showed significantly more DNA breakage when compared to uncoated ones. Uncoated FeCoB NPs and coated NPs (PAA-FeCoB NPs) also induced the formation of micronuclei. Additionally, PAA-coated NPs and uncoated FeCoB NPs showed a negative correlation between cell proliferation and DNA strand breaks, suggesting a common pathomechanism, possibly by oxidation-induced DNA damage. We conclude that uncoated FeCoB NPs are cytotoxic and genotoxic at in vitro conditions. Surface coating of FeCoB NPs with Cys and PAA does not prevent but rather aggravates DNA damage. Further safety assessment and a well-considered choice of surface coating are needed prior to application of FeCoB nanocomposites in biomedicine.

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Cell stress response to two different types of polymer coated cobalt ferrite nanoparticles

Cited by 14 publications

References 57 publications

Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Polyethylenimine-based micro/nanoparticles as vaccine adjuvants

Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

Next‐Generation Magnetic Nanocomposites: Cytotoxic and Genotoxic Effects of Coated and Uncoated Ferric Cobalt Boron (FeCoB) Nanoparticles In Vitro

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