Cell-stage-specific enhancement by caffeine of the frequency of chromatid aberrations induced by X-rays in neural ganglia of Drosophila melanogaster

Marco, A. De; Polani, Stefania

doi:10.1016/0027-5107(81)90052-x

Cited by 9 publications

(1 citation statement)

References 20 publications

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“…Many checkpoints of this type monitor DNA damage and are mediated by the ATM and ATR kinases; when activated, these checkpoints inhibit cyclin B-CDK via the inhibition of Cdc25 phosphatase (for review see Abraham, 2001 ). Thus, we filmed live gwl neuroblasts treated with levels of caffeine that disrupt these checkpoints ( De Marco and Polani, 1981 ). If such a checkpoint was activated in gwl mutants, caffeine should shorten the period of chromosome condensation before NEB.…”

Section: Resultsmentioning

confidence: 99%

Greatwall kinase

Fleming

Williams

et al. 2004

The Journal of Cell Biology

141

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Mutations in the Drosophila gene greatwall cause improper chromosome condensation and delay cell cycle progression in larval neuroblasts. Chromosomes are highly undercondensed, particularly in the euchromatin, but nevertheless contain phosphorylated histone H3, condensin, and topoisomerase II. Cells take much longer to transit the period of chromosome condensation from late G2 through nuclear envelope breakdown. Mutant cells are also subsequently delayed at metaphase, due to spindle checkpoint activity. These mutant phenotypes are not caused by spindle aberrations, by global defects in chromosome replication, or by activation of a caffeine-sensitive checkpoint. The Greatwall proteins in insects and vertebrates are located in the nucleus and belong to the AGC family of serine/threonine protein kinases; the kinase domain of Greatwall is interrupted by a long stretch of unrelated amino acids.

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Section: Resultsmentioning

confidence: 99%