Cell spread area and traction forces determine myosin-II-based cortex thickness regulation

Kumar, Rinku; Saha, Sajjita; Sinha, Bidisha

doi:10.1016/j.bbamcr.2019.07.011

Cited by 27 publications

(23 citation statements)

References 42 publications

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“…In addition to the bulk elasticity, the ES bilayer decay method allows to calculate the parameters of the AC. Some measurements of the thickness of the AC are reported, indicating a value of about 100-200nm for mitotic cells [10,23] that increases to 300-400nm in the adherent phase [60]. The measured values of d 0 where in the range 290-470nm (see Fig.5c), in line with previous results (even though obtained on different cells, and so not directly comparable).…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelsupporting

confidence: 87%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

show abstract

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelsupporting

confidence: 87%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition to the bulk elasticity, the ES bilayer decay method allows to calculate the parameters of the AC. Some measurements of the thickness of the AC are reported, indicating a value of about 100-200nm for mitotic cells [10,23] that increases to 300-400nm in the adherent phase [63]. The measured values of d 0 where in the range 290-470nm (see Fig.5c), in line with previous results (even though obtained on different cells, and so not directly comparable).…”

Section: Characterizing the Actin Cortex With The Bilayer Decay Modelsupporting

confidence: 87%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results: Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions: The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

show abstract

“…Treatment of widespread cells such as cultured muscle cells with nonmuscle myosin II inhibitors, such as blebbistatin and Y27632, was found to enhance the cortical actin network and increase its thickness ( Cai et al. , 2010 ; Kumar et al. , 2019 ).…”

Section: Resultsmentioning

confidence: 99%