Cell-Specific DNA Methylation Signatures in Asthma

Thibeault, Andrée-Anne Hudon; Laprise, Catherine

doi:10.3390/genes10110932

Cited by 35 publications

(29 citation statements)

References 152 publications

(226 reference statements)

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“…Despite the rapid progress in recent years, there are still numerous challenges for the interpretation of existing and future data. In epigenetics, it is not yet clear which tissue and cell types are best suited for analysis [114]. Most analyses have been performed in blood immune cells, but respiratory epithelial cells from the nose or bronchi have also been studied, show much better discrimination between asthmatics and controls.…”

Section: Discussionmentioning

confidence: 99%

Recent findings in the genetics and epigenetics of asthma and allergy

Kabesch¹,

Tost

2020

Semin Immunopathol

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In asthma and allergy genetics, a trend towards a few main topics developed over the last 2 years. First, a number of studies have been published recently which focus on overlapping and/or very specific phenotypes: within the allergy spectrum but also reaching beyond, looking for common genetic traits shared between different diseases or disease entities. Secondly, an urgently needed focus has been put on asthma and allergy genetics in populations genetically different from European ancestry. This acknowledges that the majority of new asthma patients today are not white and asthma is a truly worldwide disease. In epigenetics, recent years have seen several large-scale epigenome-wide association studies (EWAS) being published and a further focus was on the interaction between the environment and epigenetic signatures. And finally, the major trends in current asthma and allergy genetics and epigenetics comes from the field of pharmacogenetics, where it is necessary to understand the susceptibility for and mechanisms of current asthma and allergy therapies while at the same time, we need to have scientific answers to the recent availability of novel drugs that hold the promise for a more individualized therapy.

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Section: Discussionmentioning

confidence: 99%

Recent findings in the genetics and epigenetics of asthma and allergy

Kabesch¹,

Tost

2020

Semin Immunopathol

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“…Clearly, epigenetic regulation of gene expression is highly relevant to the translation of disease susceptibility into altered biology of the airway epithelium. Epigenetic marks are highly responsive to environmental exposures relevant to asthma inception or exacerbations, further underscoring the relevance of epigenetics for understanding asthma pathophysiology 88‐91 . Three main types of epigenetic marks can be distinguished: CpG methylation, histone modifications and small, noncoding RNAs.…”

Section: Epigenetic Factors and The Epithelial Barrier In Asthmamentioning

confidence: 99%

Epithelial cell dysfunction, a major driver of asthma development

Heijink

Kuchibhotla

Roffel

et al. 2020

Allergy

166

145

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Asthma is a chronic inflammatory airway disease characterized by coughing, wheezing, chest tightness, variable airflow limitation and airway hyper-responsiveness (AHR) 1 to environmental specific (allergens such as house dust mite (HDM), pollen and animal dander) and nonspecific (eg tobacco smoke, air pollution) stimuli. Asthma is a heterogeneous disease with a complex aetiology. Allergen-induced asthma is the most common form, with atopy and allergic sensitization being identified as major risk factors. 2 Other risk factors include increased viral infections during early childhood, exposure to tobacco smoke and air pollution. 3 In

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“…Epigenetic regulation is exerted through a plethora of chemical modifications including histones modifications and DNA methylation and plays a prominent role in a number of complex diseases 22 . Epigenetic analysis of human circulating eosinophils 23 , 24 has been reported and comparisons of DNA methylation patterns in asthmatic 25 and diabetic 26 patients has been studied, suggesting this avenue would be insightful for adipose tissue-derived eosinophils. In the current study, we describe a process to successfully analyze the genome of eosinophils derived from human adipose tissue via assessment of DNA methylation using whole genome bisulfite sequencing 27 (WGBS).…”

Section: Introductionmentioning

confidence: 99%

A FACS-based approach to obtain viable eosinophils from human adipose tissue

Hernandez

Tew

et al. 2020

Sci Rep

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Eosinophils have been widely investigated in asthma and allergic diseases. More recently, new insights into the biology of these cells has illustrated eosinophils contribute to homeostatic functions in health such as regulation of adipose tissue glucose metabolism. Human translational studies are limited by the difficulty of obtaining cells taken directly from their tissue environment, relying instead on eosinophils isolated from peripheral blood. Isolation techniques for tissue-derived eosinophils can result in unwanted cell or ribonuclease activation, leading to poor cell viability or RNA quality, which may impair analysis of effector activities of these cells. Here we demonstrate a technique to obtain eosinophils from human adipose tissue samples for the purpose of downstream molecular analysis. From as little as 2 g of intact human adipose tissue, greater than 10 4 eosinophils were purified by fluorescence-activated cell sorting (FACS) protocol resulting in ≥ 99% purity and ≥ 95% viable eosinophils. We demonstrated that the isolated eosinophils could undergo epigenetic analysis to determine differences in DNA methylation in various settings. Here we focused on comparing eosinophils isolated from human peripheral blood vs human adipose tissue. Our results open the door to future mechanistic investigations to better understand the role of tissue resident eosinophils in different context.

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Cell-Specific DNA Methylation Signatures in Asthma

Cited by 35 publications

References 152 publications

Recent findings in the genetics and epigenetics of asthma and allergy

Recent findings in the genetics and epigenetics of asthma and allergy

Epithelial cell dysfunction, a major driver of asthma development

A FACS-based approach to obtain viable eosinophils from human adipose tissue

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