Cell size and the blockage of electron transfer in photosynthesis: Proposed endpoints for algal assays and its application to soil alga Chlorococcum infusionum

Nam, Sun-Hwa; An, Youn‐Joo

doi:10.1016/j.chemosphere.2015.01.005

Cited by 25 publications

(6 citation statements)

References 61 publications

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“…However, the change in granularity was significant in strain MAS3 as compared to strain MAS1. Such a decrease in cell granularity in response to metals such as Cu and Ni was also observed in a microalga, Chlorococcum infusionum . Metals in high concentrations alter the cell membrane by increasing permeability leading to enlargement of cells due to the accumulation of photosynthetic products .…”

Section: Resultsmentioning

confidence: 60%

Sustainable Iron Recovery and Biodiesel Yield by Acid-Adapted Microalgae, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, Grown in Synthetic Acid Mine Drainage

et al. 2020

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Sustainable resource recovery is the key to manage the overburden of various waste entities of mining practices. The present study demonstrates for the first time a novel approach for iron recovery and biodiesel yield from two acid-adapted microalgae, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, grown in synthetic acid mine drainage (SAMD). Virtually, there was no difference in the growth of the strain MAS3 both in Bold’s basal medium (control) and SAMD. Using the IC50 level (200 mg L–1) and a lower concentration (50 mg L–1) of iron in SAMD, the cell granularity, exopolysaccharide (EPS) secretion, iron recovery, and biodiesel were assessed in both the strains. Both cell granularity and accumulation of EPS were significantly altered under metal stress in SAMD, resulting in an increase in total accumulation of iron. Growth of the microalgal strains in SAMD yielded 12–20% biodiesel, with no traces of heavy metals, from the biomass. The entire amount of iron, accumulated intracellularly, was recovered in the residual biomass. Our results on the ability of the acid-adapted microalgal strains in iron recovery and yield of biodiesel when grown in SAMD indicate that they could be the potential candidates for use in bioremediation of extreme habitats like AMD.

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Section: Resultsmentioning

confidence: 60%

Sustainable Iron Recovery and Biodiesel Yield by Acid-Adapted Microalgae, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, Grown in Synthetic Acid Mine Drainage

et al. 2020

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“…Similar observations have been documented for algae exposed to pollutants. After pollutant treatment (e.g., Cu; nickel, Ni), the cell size of algal cells Chlorococcum infusionum was reduced during 6 days of culture (Nam and An 2015) and the cell size was far more sensitive than other parameters of algal viability such as cell granularity and autofluorescence of chlorophyll. Similarly, Jamers et al (2009) reported that Chlamydomonas reinhardtii exhibited a decline in cell size under 5 and 100 mM Cd exposure during 72 h of culture.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, Jamers et al (2009) reported that Chlamydomonas reinhardtii exhibited a decline in cell size under 5 and 100 mM Cd exposure during 72 h of culture. The decrease in cell volume in response to Pb may be connected with toxic effect of heavy metals on the structure of cell wall and the leakage of ions or cell components through plasma membrane leading to irreversible cell damage and necrosis (Jamers et al 2009;Nam and An 2015).…”

Section: Discussionmentioning

confidence: 99%

The effect of lead on the growth, content of primary metabolites, and antioxidant response of green alga Acutodesmus obliquus (Chlorophyceae)

Piotrowska‐Niczyporuk

Bajguz

Talarek

et al. 2015

Environ Sci Pollut Res

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Green unicellular alga Acutodesmus obliquus (Turpin) Hegewald et Hanagata (SAG strain no. 276-6) (Chlorophyceae) was used for determination of phytotoxicity of lead (Pb) at the range of concentrations 0.01-500 μM during 7 days of culture. The accumulation of Pb in algal cells was found to be increased in a concentration- and duration-dependent manner. The highest Pb uptake value was obtained in response to 500 μM Pb on the seventh day of cultivation. The decrease in the number and the size of cells and the contents of selected primary metabolites (photosynthetic pigments, monosaccharides, and proteins) in A. obliquus cells were observed under Pb stress. Heavy metal stimulated also formation of reactive oxygen species (hydrogen peroxide) and oxidative damage as evidenced by increased lipid peroxidation. On the other hand, the deleterious effects of Pb resulting from the cellular oxidative state can be alleviated by enzymatic (superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase) and non-enzymatic (ascorbate, glutathione) antioxidant systems. These results suggest that A. obliquus is a promising bioindicator of heavy metal toxicity in aquatic environment, and it has been identified as good scavenger of Pb from aqueous solution.

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“…reinhardtii , an algal suspension was allowed to adapt to the dark for 15 min. The Handy Plant Efficiency Analyzer (PEA; Hansatech Instruments Ltd., Kings Lynn, Norfolk, UK) was then used to analyze Photosystem II parameters: total complementary area (area), maximum quantum yield of primary photochemistry (at t = 0) (Fv/Fm), reaction center per absorption flux (RC/ABS), reaction center per trapped energy flux (at t = 0) (RC/TRo), electron transport flux per reaction center (at t = 0) (ETo/RC), and reaction center per dissipated energy flux (at t = 0) (RC/Dio) 25 , 26 .…”

Section: Methodsmentioning

confidence: 99%

Quantification of silver nanoparticle toxicity to algae in soil via photosynthetic and flow-cytometric analyses

Nam

Kwak

2018

Sci Rep

Self Cite

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Soil algae, which have received attention for their use in a novel bioassay to evaluate soil toxicity, expand the range of terrestrial test species. However, there is no information regarding the toxicity of nanomaterials to soil algae. Thus, we evaluated the effects of silver nanoparticles (0–50 mg AgNPs/kg dry weight soil) on the soil alga Chlamydomonas reinhardtii after six days, and assessed changes in biomass, photosynthetic activity, cellular morphology, membrane permeability, esterase activity, and oxidative stress. The parameters measured were markedly affected by AgNP-induced stress at 50 mg AgNPs/kg dry weight soil, where soil algal biomass, three measures of photosynthetic activity (area, reaction center per absorption flux, and reaction center per trapped energy flux), and esterase activity decreased. AgNPs also induced increases in both cell size and membrane permeability at 50 mg AgNPs/kg dry weight soil. In addition to the increase in cell size observed via microscopy, a mucilaginous sheath formed as a protective barrier against AgNPs. Thus, the toxicity of AgNPs can be effectively quantified based on the physiological, biochemical, and morphological responses of soil algae, where quantifying the level of toxicity of AgNPs to soil algae could prove to be a useful method in terrestrial ecotoxicology.

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Cell size and the blockage of electron transfer in photosynthesis: Proposed endpoints for algal assays and its application to soil alga Chlorococcum infusionum

Cited by 25 publications

References 61 publications

Sustainable Iron Recovery and Biodiesel Yield by Acid-Adapted Microalgae, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, Grown in Synthetic Acid Mine Drainage

Sustainable Iron Recovery and Biodiesel Yield by Acid-Adapted Microalgae, Desmodesmus sp. MAS1 and Heterochlorella sp. MAS3, Grown in Synthetic Acid Mine Drainage

The effect of lead on the growth, content of primary metabolites, and antioxidant response of green alga Acutodesmus obliquus (Chlorophyceae)

Quantification of silver nanoparticle toxicity to algae in soil via photosynthetic and flow-cytometric analyses

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