Cell size and mutual cell adhesion

Abstract

HeLa cells harvested from density-inhibited or fast growing suspension cultures, were incubated in NaCl solutions of different tonicity. Cell size enlargement produced by hypotonicity is accompanied by an increased sedimentation rate of the density-inhibited cells, whereas no appreciable change is observed in the sedimentation rate of fast growing cells. Hypotonicity also has no effect on the sedimentation rate of density-inhibited cells which previously had been treated with neuraminidase or trypsin. It is shown that the effect of hypotonicity on density-inhibited cells cannot be ascribed to release of cell surface sialic acids during hypotonic incubation. Several arguments are presented which indicate that the changes in sedimentation rate, as measured in the rotating suspension system, are not the direct consequence of the alterations in cell size, but rather must be attributed to differences in intercellular adhesiveness resulting from the size alterations. Analogous changes in intercellular adhesiveness and cell size are shown to occur during growth in isotonic suspension culture. The results can be explained by assuming that changes in cell size affect the intercellular adhesiveness by modifying the extent to which cell surface sialic acids counteract adhesion.

Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System

“…These values are also in agreement with the results obtained by Deman et al. and Cartagena-Rivera et al, , of 14.5 ± 1.6 and 11.5 ± 1.5 μm, respectively.…”

Analytical and Numerical Modeling Methods for Electrochemical Impedance Analysis of Single Cells on Coplanar Electrodes

“…The gap between electrodes was 3 mm. The radius of a single HeLa cell in the simulations was assumed to be 10 mm, which is close to the size obtained from observations [29]. Figure 2a shows the FEM simulation model, including the glass substrate, gold electrodes, cell culture medium, and HeLa cell.…”

Effect of Electrode Shape on Impedance of Single HeLa Cell: A COMSOL Simulation

“…This work used the COMSOL software package for electrical characterizations of single HeLa cells with four different electrode shapes. In the simulations, the frequency range was 5 to 100 k Hz and the voltage range was 0.1 to 1 V. The assumptions in the simulations were a single HeLa cell with a diameter of 20 μ m, which approximates the actual size [ 19 , 20 ].…”