Cell sheet engineering using the stromal vascular fraction of adipose tissue as a vascularization strategy

Costa, M. Fernanda P.; Cerqueira, Mariana T.; Santos, T. C.; Sampaio‐Marques, Belém; Ludovico, Paula; Marques, Alexandra P.; Pirraco, Rogério P.; Reis, Rui L.

doi:10.1016/j.actbio.2017.03.034

Cited by 36 publications

(31 citation statements)

References 57 publications

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“…Establishment of a clonogenic/angiogenic microenvironment is a very complex problem whose solving would provide great progress in constructing implantable regeneration-supporting devices or supporting survival of grafted cells in vivo . Several attempts have been made to produce human SVF in vitro by placing ASCs and ASCs-supporting cells (pericytes, endothelial cells) on perfused scaffolds ( Scherberich et al, 2010 ; Cerino et al, 2017 ; Costa et al, 2017 ). The results confirmed strong angiogenic potential of ASCs and release of proangiogenic factors and development of vascular network ( Cerino et al, 2017 ; Costa et al, 2017 ).…”

Section: Conclusion and Perspectives: Novel Vascular Engineering Stramentioning

confidence: 99%

“…Several attempts have been made to produce human SVF in vitro by placing ASCs and ASCs-supporting cells (pericytes, endothelial cells) on perfused scaffolds ( Scherberich et al, 2010 ; Cerino et al, 2017 ; Costa et al, 2017 ). The results confirmed strong angiogenic potential of ASCs and release of proangiogenic factors and development of vascular network ( Cerino et al, 2017 ; Costa et al, 2017 ). Recently, tissue-engineered vascular grafts with BADSCs have been proposed as promising novel alternatives to replace diseased vessels in cardiovascular and cerebrovascular diseases ( Wang et al, 2016 , 2017 ).…”

Section: Conclusion and Perspectives: Novel Vascular Engineering Stramentioning

confidence: 99%

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Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

et al. 2018

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Adipose tissue is recognized as an important organ with metabolic, regulatory, and plastic roles. Adipose tissue-derived stem cells (ASCs) with self-renewal properties localize in the stromal vascular fraction (SVF) being present in a vascular niche, thereby, contributing to local regulation of angiogenesis and vessel remodeling. In the past decades, ASCs have attracted much attention from biologists and bioengineers, particularly, because of their multilineage differentiation potential, strong proliferation, and migration abilities in vitro and high resistance to oxidative stress and senescence. Current data suggest that the SVF serves as an important source of endothelial progenitors, endothelial cells, and pericytes, thereby, contributing to vessel remodeling and growth. In addition, ASCs demonstrate intriguing metabolic and interlineage plasticity, which makes them good candidates for creating regenerative therapeutic protocols, in vitro tissue models and microphysiological systems, and tissue-on-chip devices for diagnostic and regeneration-supporting purposes. This review covers recent achievements in understanding the metabolic activity within the SVF niches (lactate and NAD+ metabolism), which is critical for maintaining the pool of ASCs, and discloses their pro-angiogenic potential, particularly, in the complex therapy of cardiovascular and cerebrovascular diseases.

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Section: Conclusion and Perspectives: Novel Vascular Engineering Stramentioning

confidence: 99%

Section: Conclusion and Perspectives: Novel Vascular Engineering Stramentioning

confidence: 99%

Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

et al. 2018

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“…It is interesting to note that the SVF from which ASCs are isolated also contains angiogenic cell populations such as endothelial cells, endothelial progenitor cells and pericytes (72). These constitute a perfect cocktail of cells to create functional vasculature in bone TE constructs, therefore addressing one of the main current obstacles in TE (73,74). The possibility of using the whole fraction both for osteogenesis and angiogenesis is extremely exciting and will allow from one simple procedure to obtain patient-specific cell population for a complete bone TE strategy.…”

Section: Stem Cells For Engineering Bonementioning

confidence: 99%

Stem Cells for Osteochondral Regeneration

Canadas

Pirraco

Oliveira

et al. 2018

Osteochondral Tissue Engineering

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Stem cell research plays a central role in the future of medicine, which is mainly dependent on the advances on regenerative medicine (RM), specifically in the disciplines of tissue engineering (TE) and cellular therapeutics. All RM strategies depend upon the harnessing, stimulation, or guidance of endogenous developmental or repair processes in which cells have an important role. Among the most clinically challenging disorders, cartilage degeneration, which also affects subchondral bone becoming an osteochondral (OC) defect, is one of the most demanding. Although primary cells have been clinically applied, stem cells are currently seen as the promising tool of RM-related research because of its availability, in vitro proliferation ability, pluri- or multipotency, and immunosuppressive features. Being the OC unit, a transition from the bone to cartilage, mesenchymal stem cells (MSCs) are the main focus for OC regeneration. Promising alternatives, which can also be obtained from the patient or at banks and have great differentiation potential toward a wide range of specific cell types, have been reported. Still, ethical concerns and tumorigenic risk are currently under discussion and assessment. In this book chapter, we revise the existing stem cell-based approaches for engineering bone and cartilage, focusing on cell therapy and TE. Furthermore, 3D OC composites based on cell co-cultures are described. Finally, future directions and challenges still to be faced are critically discussed.

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“…Regarding angiogenesis, the authors previously reported that the transplantation of artificial skin with aldehyde dehydrogenase‐positive cells in SVF to rat skin defects, and the cells were found to differentiate into blood vessels in vivo . Furthermore, Costa et al reported that (1) improved blood flow was found in a mouse hind‐limb ischemia model after angiogenic cell sheets developed from SVF were transplanted and (2) increased secretion of angiogenic factors was observed by SVF under hypoxic conditions . Conversely, SVF shows immunosuppressive activity through macrophages.…”

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confidence: 99%

Adipose‐derived stem cells and the stromal vascular fraction in polyglycolic acid‐collagen nerve conduits promote rat facial nerve regeneration

Shimizu

Matsumine

Osaki

et al. 2018

Wound Repair Regeneration

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Adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF) promote nerve regeneration. Biodegradable nerve conduits are used to treat peripheral nerve injuries, but their efficiencies are lower than those of autologous nerve grafts. This study developed biodegradable nerve conduits containing ADSCs and SVF and evaluated their facial nerve regenerating abilities in a rat model with a 7-mm nerve defect. SVF and ADSCs were individually poured into nerve conduits with polyglycolic acid-type I collagen as a scaffold (ADSCs and SVF groups). The conduits were grafted on to the nerve defects. As the control, the defect was bridged with polyglycolic acid-collagen nerve conduits without cells. At 13 weeks, after transplantation, the regenerated nerves were evaluated physiologically and histologically. The compound muscle action potential of the SVF group was significantly higher in amplitude than that of the control group. Electron microscopy showed that the axon diameter of the SVF group was the largest, followed by the ADSC group and control group with significant differences among them. The SVF group had the largest fiber diameter, followed by the ADSC group and control group with significant differences among them. The ADSC group had the highest myelin thickness, followed by the SVF group and control group with significant differences among them. Identical excellent promoting effects on nerve regeneration were observed in both the ADSC and SVF groups. Using SVF in conduits was more practical than using ADSCs because only the enzymatic process was required to prepare SVF, indicating that SVF could be more suitable to induce nerve regeneration.

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Cell sheet engineering using the stromal vascular fraction of adipose tissue as a vascularization strategy

Cited by 36 publications

References 57 publications

Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

Plasticity of Adipose Tissue-Derived Stem Cells and Regulation of Angiogenesis

Stem Cells for Osteochondral Regeneration

Adipose‐derived stem cells and the stromal vascular fraction in polyglycolic acid‐collagen nerve conduits promote rat facial nerve regeneration

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