Cell sheet-based multilayered liver tumor models for anti-cancer drug screening

Yang, Jianing; Zhao, Shengjun; Ji, Yunfei; Zhao, Lili; Kong, Qingzhu; Zhang, Qing

doi:10.1007/s10529-017-2476-1

Cited by 11 publications

(7 citation statements)

References 10 publications

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“…[1] To overcome these limitations and to achieve better replication of the in vivo tumor characteristics, 3D tumor models based on cell aggregates have emerged as a promising alternative in mimicking the sophisticated spatial arrangements of the cells for predicting the efficacy of and resistance against antitumor agents toward drug evaluation and cancer research. [1e,2] To this end, numerous fabrication strategies have been reported for generating cell aggregates, such as cell sheet engineering, [3] hanging drop method, [4] and microwell plate culture. [5] More recently, tremendous progress has evidenced the applications of 3D scaffold-based models toward tumor tissue engineering and diagnostics.…”

Section: Introductionmentioning

confidence: 99%

“…To this end, numerous fabrication strategies have been reported for generating cell aggregates, such as cell sheet engineering, [ 3 ] hanging drop method, [ 4 ] and microwell plate culture. [ 5 ] More recently, tremendous progress has evidenced the applications of 3D scaffold‐based models toward tumor tissue engineering and diagnostics.…”

Section: Introductionmentioning

confidence: 99%

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Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

et al. 2020

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Compared to various traditional 2D approaches, the scaffold‐based 3D tumor models have emerged as an effective strategy to investigate the complex mechanisms behind cancer progression and responses to drug treatments, by providing biomimetic extracellular matrix and stromal‐like microenvironments including the vascular elements. Herein, the development of a 3D endothelialized hepatic tumor microtissue model based on the fusion of multicellular aggregates of human hepatocellular carcinoma cells and human umbilical vein endothelial cells cocultured in poly(lactic‐ co ‐glycolic acid)‐based porous microspheres (PLGA PMs) is reported. In contrast to the conventional 2D culture, the cells within the PLGA PMs exhibit significantly higher half‐maximal inhibitory concentration values against anticancer drugs, including doxorubicin and cisplatin. Furthermore, the feasibility of coculturing other cell types, such as fibroblasts (L929) and HepG2 cells, is investigated. Together, the findings emphasize the significance of engineered 3D hepatic tumor microtissue models using PLGA PM‐based multicellular aggregates for drug screening applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

et al. 2020

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“…Recently, 3D tissue models have been widely used for drug screening in research institutions and drug development companies. 67 However, cell necrosis can sometimes happen due to the limited oxygen and nutrients inside multi-layered cell sheets. 29…”

Section: F I G U R E 5 Reactive Oxygen Species (Ros)-induced Cell She...mentioning

confidence: 99%

Tailoring cell sheets for biomedical applications

Chen,

Nie,

Gan

et al. 2024

Smart Medicine

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Cell sheet technology has emerged as a novel scaffold‐free approach for cell‐based therapies in regenerative medicine. Techniques for harvesting cell sheets are essential to preserve the integrity of living cell sheets. This review provides an overview of fundamental technologies to fabricate cell sheets and recent advances in cell sheet‐based tissue engineering. In addition to the commonly used temperature‐responsive systems, we introduce alternative approaches, such as ROS‐induced, magnetic‐controlled, and light‐induced cell sheet technologies. Moreover, we discuss the modification of the cell sheet to improve its function, including stacking, genetic modification, and vascularization. With the significant advances in cell sheet technology, cell sheets have been widely applied in various tissues and organs, including but not limited to the lung, cornea, cartilage, periodontium, heart, and liver. This review further describes both the preclinical and clinical applications of cell sheets. We believe that the progress in cell sheet technology would further propel its biomedical applications.

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“…Cell types in this strategy, however, are limited to those that are adherent cell cultures. As cell sheets are harvested from confluent monolayer cultures, their resolutions are typically within the scale of single cell size, often producing membranes less than 100 μm in thickness. , Cell sheets have been applied to various tissues, including bone, cornea, cardiac, cartilage, liver, kidney, esophagus, lung, middle ear mucosa, urethra, small intestine, and tendon. − A summary of these applications is provided in Table , but some are further detailed in this section.…”

Section: Fabrication Strategiesmentioning

confidence: 99%

Fabrication Strategies for Engineered Thin Membranous Tissues

McLoughlin

McKenna

Fisher

2023

ACS Appl. Bio Mater.

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Thin membranous tissues (TMTs) are anatomical structures consisting of multiple stratified cell layers, each less than 100 μm in thickness. While these tissues are small in scale, they play critical roles in normal tissue function and healing. Examples of TMTs include the tympanic membrane, cornea, periosteum, and epidermis. Damage to these structures can be caused by trauma or congenital disabilities, resulting in hearing loss, blindness, dysfunctional bone development, and impaired wound repair, respectively. While autologous and allogeneic tissue sources for these membranes exist, they are significantly limited by availability and patient complications. Tissue engineering has therefore become a popular strategy for TMT replacement. However, due to their complex microscale architecture, TMTs are often difficult to replicate in a biomimetic manner. The critical challenge in TMT fabrication is balancing fine resolution with the ability to mimic complex target tissue anatomy. This Review reports existing TMT fabrication strategies, their resolution and material capabilities, cell and tissue response, and the advantages and disadvantages of each technique.

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Cell sheet-based multilayered liver tumor models for anti-cancer drug screening

Abstract: CSLTMs showed a thickness-dependent cytotoxic efficacy of DOX and greater drug resistance than the control, thereby providing useful information toward the development of improved biomimetic tumor models.

Cited by 11 publications

References 10 publications

Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

Modeling Endothelialized Hepatic Tumor Microtissues for Drug Screening

Tailoring cell sheets for biomedical applications

Fabrication Strategies for Engineered Thin Membranous Tissues

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