Cell sheet approach for tissue engineering and regenerative medicine

Matsuura, Kiyotaka; Utoh, Rie; Nagase, Kenichi; Okano, Teruo

doi:10.1016/j.jconrel.2014.05.024

Cited by 207 publications

(167 citation statements)

References 129 publications

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“…6,7 The cell sheets could be applied layer by layer and could attach well to defective areas of tissues, allowing for either direct in vivo transplantation or combination with biomaterials in tissue engineering. 8 This technique has proved effective in treating esophageal ulcerations, 9 periodontal tissue destruction, 3 corneal dysfunction, 10 myocardial infarction, 2 and so on.…”

mentioning

confidence: 99%

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Wang

Wu²,

Wei

et al. 2016

IJN

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Cell sheet engineering has emerged as a novel approach to effectively deliver seeding cells for tissue regeneration, and developing human bone marrow mesenchymal stem cell (hBMMSC) sheets with high osteogenic ability is a constant requirement from clinics for faster and higher-quality bone formation. In this work, we fabricated biocompatible and safe chitosan (CS)/hyaluronic acid (HA) nanoparticles (NPs) to deliver microRNA-21 (miR-21), which has been proved to accelerate osteogenesis in hBMMSCs; then, the CS/HA/miR-21 NPs were cross-linked onto the surfaces of culture plates with 0.2% gel solution to fabricate miR-21-functionalized culture plates for reverse transfection. hBMMSC sheets were induced continuously for 14 days using a vitamin C-rich method on the miR-21-functionalized culture plates. For the characterization of CS/HA/miR-21 NPs, the particle size, zeta potential, surface morphology, and gel retardation were sequentially investigated. Then, the biological effects of hBMMSC sheets on the miR-21-functionalized culture plates were evaluated. The assay results demonstrated that the hBMMSC sheets could be successfully induced via the novel reverse transfection approach, and miR-21 delivery significantly enhanced the in vitro osteogenic differentiation of hBMMSC sheets in terms of upregulating calcification-related gene expression and enhancing alkaline phosphatase production, collagen secretion, and mineralized nodule formation. The enhanced osteogenic activity of hBMMSC sheets might promisingly lead to more rapid and more robust bone regeneration for clinical use.

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mentioning

confidence: 99%

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Wang

Wu²,

Wei

et al. 2016

IJN

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“…It leads to promotion of tissue repair. 12 Further, process of integration of cells and sufficient vascularization opened so many possibilities for fabrication of human 3-dimensional vascularized dense and intact tissue grafts for regenerative medicine to parenchymal organs. 12 This new technology has increased rate of clinical successes and made curing patients with difficult-to-treat diseases and physically impaired function.…”

Section: Biological Scaffoldsmentioning

confidence: 99%

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Upadhyay¹

2017

ATROA

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Present review article emphasizes role of biological scaffolds, hydrogels and stem cells in tissue engineering mainly in regeneration or repairing of damaged tissues. Highly porous scaffold biomaterials are developed which act as templates for tissue regeneration and potentially guide the growth of new tissue. Present article also describes de-cellularization, cell printing, vascularization, integration of cross linking of methods for scaffolding of biomaterials to reproduce and recapitulate complexity in engineered tissues. It also explains different scaffold types, polymer hydrogels which are necessary for formation of microstructure, cell attachment, differentiation, tissue vascularization and integration. It also explains use of induced pluripotent stem cells (iPSCs) and engineered MSCs as new diagnostic and potential therapeutic tools to remove sustained damage and complications from organ failures. These engineered MSCs assist in making self-assembling supramolecular hydrogels, which have larger applications in cell therapy of intractable diseases and tissue regeneration. No doubt development of more advanced biomaterials, growth factors and stem cell derived products/factors and tissue transplantation methods based on cell regeneration programming will revolutionize the clinical therapeutics. This article suggests identification of new biomaterials/biomolecules such as growth factors, scaffolds, integration, adhesion and regulatory molecules and cell secreted factors which can induce major metabolic and signaling pathways during phase of tissue repairing and induction of regeneration. This innovative research area needs many conceptual improvements in organ therapies, methods and technological advancement to scale more services to the human society.

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“…CS can be used to cover a signiicant surface making it a good technique for supericial lesions, cardiomyoplasty and ophthalmologic and microsurgical manipulations. Numerous clinical trials are being run in Japan these years to reveal their full potential in a wide array of disorders [109].…”

Section: Cell Sheets: Minimal Tissue-engineered Constructsmentioning

confidence: 99%

Therapeutic Angiogenesis: Foundations and Practical Application

Макаревич¹,

Parfyonova²

2017

Physiologic and Pathologic Angiogenesis - Signaling Mechanisms and Targeted Therapy

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Angiogenesis as therapeutic target has emerged since early works by Judah Folkman, yet his "holy grail" was inhibiting vascular growth to block tumor nutrition. However, in modern biomedicine, "therapeutic angiogenesis" became a large ield focusing on stimulation of blood vessel growth for ischemia relief to reduce its detrimental efects in the tissues. In this review, we introduce basic principles of tissue vascularization in response to ischemia exploited in this ield. An overview of recent status in therapeutic angiogenesis is given with introduction to emerging technologies, including gene therapy, genetic modiication of cells ex vivo and tissue engineering.

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Cell sheet approach for tissue engineering and regenerative medicine

Cited by 207 publications

References 129 publications

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Improving the osteogenesis of human bone marrow mesenchymal stem cell sheets by microRNA-21-loaded chitosan/hyaluronic acid nanoparticles via reverse transfection

Role of Biological Scaffolds, Hydro Gels and Stem Cells in Tissue Regeneration Therapy

Therapeutic Angiogenesis: Foundations and Practical Application

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