Cell Shape and Cell Lineage Conversion

Nakaya, Yukiko; Sheng, Guojun

doi:10.2141/jpsa.0140088

Cited by 3 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EMT is essential for embryonic development, although it induces fibrosis and carcinoma generation 45 , 46 . We observed expression of EMT regulators in ribosome-induced cell clusters; however, it remains unclear whether cellular regulation and function were affected by the HDF phenotype.…”

Section: Discussionmentioning

confidence: 99%

Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency

Ito

Katoh

Kushige

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Recently, we reported that bacterial incorporation induces cellular transdifferentiation of human fibroblasts. However, the bacterium-intrinsic cellular- transdifferentiation factor remained unknown. Here, we found that cellular transdifferentiation is caused by ribosomes. Ribosomes, isolated from both prokaryotic and eukaryotic cells, induce the formation of embryoid body-like cell clusters. Numerous ribosomes are incorporated into both the cytoplasm and nucleus through trypsin-activated endocytosis, which leads to cell-cluster formation. Although ribosome-induced cell clusters (RICs) express several stemness markers and differentiate into derivatives of all three germ layers in heterogeneous cell populations, RICs fail to proliferate, alter the methylation states of pluripotent genes, or contribute to teratoma or chimera formation. However, RICs express markers of epithelial–mesenchymal transition without altering the cell cycle, despite their proliferation obstruction. These findings demonstrate that incorporation of ribosomes into host cells induces cell transdifferentiation and alters cellular plasticity.

show abstract

Section: Discussionmentioning

confidence: 99%

Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency

Ito

Katoh

Kushige

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Though, the molecular mechanism by which the expression of Snail and TGF-β1 were reduced is unclear, it is certain that the cell clusters transdifferentiated into mesenchymal-like cells. However, the morphology of cell cluster constitutive cells were difficult to categorize as mesenchymal cells [Nakaya and Sheng, 2015]. It is also necessary to analyze the relationship between cell structure and ribosome incorporation.…”

Section: Discussionmentioning

confidence: 99%

Ribosome Incorporation Induces EMT-like Phenomenon with Cell Cycle Arrest in Human Breast Cancer Cell

Kudo

Anam

Istiaq

et al. 2021

Cells Tissues Organs

View full text Add to dashboard Cite

Although ribosomes are generally known to be a translational machinery, some ribosomal proteins also have accessory functions involving early development and differentiation. Previously, we reported that ribosome incorporation into human dermal fibroblasts generated embryoid body-like cell clusters, altered cellular fate, and differentiated into cells of all 3 germ layers. However, the molecular phenomena induced by ribosome incorporation in the cell remained unknown. Here, we demonstrate that ribosome incorporation into human breast cancer cell MCF7 leads to ribosome-induced cell clusters (RICs) formation accompanying with epithelial-mesenchymal transition (EMT)-like gene expression. Following ribosome incorporation, MCF7 cells cease proliferation, which is caused by inhibition of cell cycle transition from G0 to G1 phase. Further, MCF7 RICs show induced expression of EMT markers, TGF-β1 and Snail along with autophagy markers and tumor suppressor gene p53. These findings indicate that the incorporation of ribosome into cancer cells induces an EMT-like phenomenon and changes the cancer cell characteristics.

show abstract

“…), that possess stemness properties (Mani et al . ; Nakaya & Sheng ). To summarize this process, it is hypothesized that H. pylori induces CDX1 expression, which in turn causes dedifferentiation of epithelial cells, resulting in intestinal metaplasia that converts the gastric epithelial cells into intestine‐like cells.…”

Section: Cellular Reprogramming By Helicobacter Pylorimentioning

confidence: 99%

“…KLF5 and SALL4 are involved in the maintenance of pluripotent markers of stem cells (Zhang et al 2006). H. pylori infected gastric epithelial cells generate epithelial-mesenchymal transition (EMT) cells (Bessede et al 2014), that possess stemness properties (Mani et al 2008;Nakaya & Sheng 2014). To summarize this process, it is hypothesized that H. pylori induces CDX1 expression, which in turn causes dedifferentiation of epithelial cells, resulting in intestinal metaplasia that converts the gastric epithelial cells into intestine-like cells.…”

Section: Cellular Reprogramming By Helicobacter Pylorimentioning

confidence: 99%

Reprogramming of human somatic cells by bacteria

Ito

Ohta

2015

Dev Growth Differ

View full text Add to dashboard Cite

In general, it had been believed that the cell fate restriction of terminally differentiated somatic cells was irreversible. In 1952, somatic cell nuclear transfer (SCNT) was introduced to study early embryonic development in frogs. So far, various mammalian species have been successfully cloned using the SCNT technique, though its efficiency is very low. Embryonic stem (ES) cells were the first pluripotent cells to be isolated from an embryo and have a powerful potential to differentiate into more than 260 types of cells. The generation of induced pluripotent stem (iPS) cells was a breakthrough in stem cell research, and the use of these iPS cells has solved problems such as low efficiency and cell fate restriction. These cells have since been used for clinical application, disease investigation, and drug selection. As it is widely accepted that the endosymbiosis of Archaea into eukaryotic ancestors resulted in the generation of eukaryotic cells, we examined whether bacterial infection could alter host cell fate. We previously showed that when human dermal fibroblast (HDF) cells were incorporated with lactic acid bacteria (LAB), the LAB-incorporated HDF cells formed clusters and expressed a subset of common pluripotent markers. Moreover, LAB-incorporated cell clusters could differentiate into cells derived from each of the three germinal layers both in vivo and in vitro, indicating successful reprogramming of host HDF cells by LAB. In the current review, we introduce the existing examples of cellular reprogramming by bacteria and discuss their nuclear reprogramming mechanisms.

show abstract

Cell Shape and Cell Lineage Conversion

Cited by 3 publications

References 43 publications

Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency

Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency

Ribosome Incorporation Induces EMT-like Phenomenon with Cell Cycle Arrest in Human Breast Cancer Cell

Reprogramming of human somatic cells by bacteria

Contact Info

Product

Resources

About