Cell separation by expanded bed adsorption: use of ion exchange chromatography for the separation of E. coli and S. cerevisiae

Ujam, L. B.; Clemmitt, R. H.; Chase, Howard A.

doi:10.1007/s004499900160

Cited by 2 publications

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“…Molecular imprinting is a new potential separation technique for preparing specialized recognition polymers and it has received much attention in recent years (Chang and Chase, 1996;Hjorth, 1997;Ujam et al, 2000;Demchenko, 2001). This potential technology is a method for making selective binding sites in synthetic polymers by using a molecular template.…”

Section: Introductionmentioning

confidence: 99%

Application of surface molecular imprinting adsorbent in expanded bed for the adsorption of Ni2+ and adsorption model

et al. 2007

Journal of Environmental Management

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Section: Introductionmentioning

confidence: 99%

Application of surface molecular imprinting adsorbent in expanded bed for the adsorption of Ni2+ and adsorption model

et al. 2007

Journal of Environmental Management

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“…The advantages of expanded beds, including the high interparticular voidage, high adsorbent surface area, and low shear hydrodynamic environment, make this contactor particularly well suited to the separation of delicate cells. Indeed, an expanded-bed procedure for the selective separation of E. coli and S. cerevisiae cells using ion-exchange chromatography has recently been reported (Ujam et al, 2000). However, the adsorption of microbial cells avoids many of the cellular processes such as receptor diffusion, cell membrane fragility, receptor up-and downregulation, and complex secondary responses following binding for which engineering solutions must be developed for any successful device.…”

Section: Introductionmentioning

confidence: 99%

Isolation of monocytes from human peripheral blood using immuno‐affinity expanded‐bed adsorption

Ujam

Clemmitt

Clarke

et al. 2003

Biotech & Bioengineering

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A novel technique for the separation of monocytes from human peripheral blood preparations has been developed. The technique is based on the use of expanded-bed adsorption and a solid perfluorocarbon derivatized with avidin or streptavidin for the indirect positive or negative capture of cells labeled with biotinylated monoclonal antibodies. The perfluorocarbon support was prepared and characterized and the contactor design and operating conditions, that enable cells to be selectively isolated, were investigated. Experiments consisted of applying an immunolabeled pulse of 1 x 10(8) peripheral blood mononuclear cells (PBMCs), isolated by density gradient centrifugation, directly onto a refrigerated expanded bed. The major cell types remaining were T-lymphocytes, B-lymphocytes, and monocytes. Monocytes could be positively adsorbed, following labeling with anti-CD14 mAb, with a clearance of up to 89% and a depletion factor of 7.6. They could also be "eluted" using mechanical shear, with a 77% yield of the applied cells at a purity of 90% and >/= 65% viability. Negative isolation of monocytes, following labeling of the other cells present with anti-CD2, CD7, CD16, CD19, and CD56 mAbs, resulted in lymphocyte depletions of up to 81% with a monocyte enrichment factor of 3.8 and purity of 71%. The monocyte viability in the flowthrough was assessed to be > 95%. This combination of expanded-bed adsorption and fluidizable affinity supports shows significant potential for the intensification of cell separations.

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Cell separation by expanded bed adsorption: use of ion exchange chromatography for the separation of E. coli and S. cerevisiae

Cited by 2 publications

References 0 publications

Application of surface molecular imprinting adsorbent in expanded bed for the adsorption of Ni2+ and adsorption model

Application of surface molecular imprinting adsorbent in expanded bed for the adsorption of Ni2+ and adsorption model

Isolation of monocytes from human peripheral blood using immuno‐affinity expanded‐bed adsorption

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