Cell separation: a review

Kumar, Rakesh; Lykke, A. W. J.

doi:10.3109/00313028409067911

Cited by 29 publications

(13 citation statements)

References 91 publications

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“…Many groups over the past decades have proposed unique microfluidic geometries designed to improve the magnetic separation of cells (Melville et al 1975; Kumar and Lykke 1984; Zborowski et al 1999), particles (Gerber 1984; Lien et al 2007; Wu et al 2011; Zhu et al 2011; Verbruggen et al 2015) and other suspensions. A common practice involves attaching magnetic particles to target cells, proteins, fungi, or viruses to improve separation efficiency (Hayes et al 2001; Furdui and Harrison 2004; Inglis et al 2004; Kim and Park 2005; Pamme et al 2006; Xia et al 2006; Chen et al 2007; Hahn et al 2007; Earhart et al 2009; Yung et al 2009; Kang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Design of microfluidic channels for magnetic separation of malaria-infected red blood cells

Martin

Gandini

et al. 2016

Microfluid Nanofluid

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This study is motivated by the development of a blood cell filtration device for removal of malaria-infected, parasitized red blood cells (pRBCs). The blood was modeled as a multi-component fluid using the computational fluid dynamics discrete element method (CFD-DEM), wherein plasma was treated as a Newtonian fluid and the red blood cells (RBCs) were modeled as soft-sphere solid particles which move under the influence of drag, collisions with other RBCs, and a magnetic force. The CFD-DEM model was first validated by a comparison with experimental data from Han et al. 2006 (Han and Frazier 2006) involving a microfluidic magnetophoretic separator for paramagnetic deoxygenated blood cells. The computational model was then applied to a parametric study of a parallel-plate separator having hematocrit of 40% with a 10% of the RBCs as pRBCs. Specifically, we investigated the hypothesis of introducing an upstream constriction to the channel to divert the magnetic cells within the near-wall layer where the magnetic force is greatest. Simulations compared the efficacy of various geometries upon the stratification efficiency of the pRBCs. For a channel with nominal height of 100 µm, the addition of an upstream constriction of 80% improved the proportion of pRBCs retained adjacent to the magnetic wall (separation efficiency) by almost 2 fold, from 26% to 49%. Further addition of a downstream diffuser reduced remixing, hence improved separation efficiency to 72%. The constriction introduced a greater pressure drop (from 17 to 495 Pa), which should be considered when scaling-up this design for a clinical-sized system. Overall, the advantages of this design include its ability to accommodate physiological hematocrit and high throughput – which is critical for clinical implementation as a blood-filtration system.

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Section: Discussionmentioning

confidence: 99%

Design of microfluidic channels for magnetic separation of malaria-infected red blood cells

Martin

Gandini

et al. 2016

Microfluid Nanofluid

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“…1). The supernatant was then carefully poured off, leaving the cell pellet in the bottom of the tube 12−17 …”

Section: Methodsmentioning

confidence: 99%

New technique to assess the axilla for breast cancer metastases using cell separation technology

Edwards¹,

Twin²,

Wilkinson³

2002

ANZ Journal of Surgery

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“…The principle of operation of this device is similar to that used for sorting cells in a variety of medical applications, including diagnostics, experimental analyses and the removal of other biohazards from blood [5][6][7]. This principle was first applied to malaria-infected blood by Paul et al [8] which has motivated the investigations of magnetic isolation of in-vitro grown malaria-infected cells (pRBCs) [8][9][10][11].…”

Section: Introductionmentioning

confidence: 97%

Evaluation of optimization algorithms for the design of a magnetic cell separator for malaria-infected blood

Kim

2015

J Mech Sci Technol

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Recently, a dialysis-like filtration device has been devised to remove the red blood cells infected with the malaria parasite from whole blood using magnetic separation techniques. The capturing efficiency of this process depends on many interrelated design variables and constraints such as magnetic pole array pitch, channel height, length and flow rate. In this study, these parameters are varied in several common optimization algorithms, so as to minimize the channel length while maximizing the capturing efficiency. The equations of motion are solved numerically to predict the trajectories of the infected cells within an idealized rectangular micro-channel incorporating periodic high gradient magnetic poles. The channel length of the optimized design is obtained, together with the associated capturing efficiency. The optimization of the channel geometry and flow rate improves the efficiency of the device.

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Cell separation: a review

Cited by 29 publications

References 91 publications

Design of microfluidic channels for magnetic separation of malaria-infected red blood cells

Design of microfluidic channels for magnetic separation of malaria-infected red blood cells

New technique to assess the axilla for breast cancer metastases using cell separation technology

Evaluation of optimization algorithms for the design of a magnetic cell separator for malaria-infected blood

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