Cell seeding in porous transplantation devices

Wald, Heidi L.; Sarakinos, Georgios; Lyman, Michelle D.; Mikos, Antonios G.; Vacanti, Joseph P.; Langer, Robert

doi:10.1016/0142-9612(93)90117-k

Cited by 117 publications

(51 citation statements)

References 6 publications

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“…Our experience agrees with literature data indicating that porous materials promote cell growth and induce the production of extracellular matrix components by the cells [21,25]. A uniform distribution and pore interconnections are important to facilitate the formation of tissues in the form of an organized network, with a wide range of applications in tissue reconstruction [3,26,27]. In vivo, porosity and pore interconnection are essential for the proliferation of vessels, facilitating tissue nutrition around the implant.…”

Section: Bioresorbable Polymers For Tissue Engineering 229supporting

confidence: 80%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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Polymeric biomaterials are used as substitutes for damaged tissue and for the stimulation of tissue regeneration. One class of polymeric biomaterials are bioresorbable polymers that degrade both in vitro and in vivo and are used as a temporary support for tissue regeneration. Among the various types of bioresorbable polymers, -hydroxy acids including the different forms of poly(lactic acid) (PLA), such as poly(L-lactic acid), poly(Dlactic acid) and poly(DL-lactic acid), as well as poly(glycolic acid) and polycaprolactone, have been extensively studied. These polymers are well known for their good biocompatibility, with their degradation products being eliminated from the body by metabolic pathways. Many reports have shown that the different PLA-based substrates do not present toxicity since the cells were found to differentiate over the different polymers, as demonstrated by the production of extracellular matrix components by various cell types. In this chapter, we describe the use of -hydroxy acids, highlighting the different forms of PLA scaffolds used as cell culture substrates and their applications in clinical practice. The chapter is divided into (1) Introduction; (2) Bioresorbable devices as cell culture substrates; (3) Cell adhesion to polymer substrates; (4) Tissue engineering and bioresorbable polymers; (5) Cell growth and proliferation on bioresorbable polymers; (6) Bioresorbable polymers for cartilage engineering; (7) Bioresorbable polymers for bone tissue engineering; (8) Bioresorbable polymers for skin tissue engineering, and (9) Conclusion.

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Section: Bioresorbable Polymers For Tissue Engineering 229supporting

confidence: 80%

Bioresorbable Polymers for Tissue Engineering

Santos¹

2010

Tissue Engineering

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“…They were also able to enter into pores, creating three-dimensional arrangements on the blend surface, even on those samples where cell affinity for the pores was smaller. The uniform distribution and interconnection of porous structures are important for cell migration and the formation of organized network structures similar to a tissue (26). A similar growth pattern of Vero cells on different polymers was previously reported.…”

Section: Discussionmentioning

confidence: 49%

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

Santos

Ferreira

Duek

et al. 2005

Braz J Med Biol Res

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Vero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37°C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, 40/60, and 0/100). The present study evaluated morphology of cells grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis, and stained with Toluidine blue, pH 4.0, and Xylidine ponceau, pH 2.5. Our results showed that cell adhesion was better when 60/40 and 50/50 blends were used although cells were able to grow and proliferate on all blends tested. When using PLLA/PHBV (50/50) slightly flattened cells were observed on porous and smooth areas. PLLA/PHBV (40/60) blends presented flattened cells on smooth areas. PLLA/PHBV (0/100), which presented no pores, also supported spreading cells interconnected by thin filaments. Histological sections showed that cells grew as a confluent monolayer on different substrates. Cytochemical analysis showed basophilic cells, indicating a large amount of RNA and proteins. Hence, we detected changes in cell morphology induced by alterations in blend proportions. This suggests that the cells changed their differentiation pattern when on various PLLA/PHBV blend surfaces. Correspondence

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“…Aliphatic polyesters, such as poly(lactic acid) (PLA), poly(glycolic acid), and their copolymers have been commonly used as matrices for cell cultures [107][108][109]. These conventional biodegradable polymers are usually highly hydrophobic that hinders penetration of the matrixes by the cell culture medium [110]. Furthermore, significant and non-specific protein adsorption on conventional polymer surfaces makes it difficult to regulate the functions of adherent cells.…”

Section: Cell-function Controllable Surfacesmentioning

confidence: 99%

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

Iwasaki

Ishihara

2012

Science and Technology of Advanced Materials

260

216

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This review article describes fundamental aspects of cell membrane-inspired phospholipid polymers and their usefulness in the development of medical devices. Since the early 1990s, polymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units have been considered in the preparation of biomaterials. MPC polymers can provide an artificial cell membrane structure at the surface and serve as excellent biointerfaces between artificial and biological systems. They have also been applied in the surface modification of some medical devices including long-term implantable artificial organs. An MPC polymer biointerface can suppress unfavorable biological reactions such as protein adsorption and cell adhesion -in other words, specific biomolecules immobilized on an MPC polymer surface retain their original functions. MPC polymers are also being increasingly used for creating biointerfaces with artificial cell membrane structures.

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Cell seeding in porous transplantation devices

Cited by 117 publications

References 6 publications

Bioresorbable Polymers for Tissue Engineering

Bioresorbable Polymers for Tissue Engineering

Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture

Cell membrane-inspired phospholipid polymers for developing medical devices with excellent biointerfaces

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