Cell-seeded porous silk fibroin scaffolds promotes axonal regeneration and myelination in spinal cord injury rats

You, Kemin; Chang, Hongze; Zhang, Feng; Shen, Yixin; Yan, Zhang; Cai, Feng; Liu, Liang; Liu, Xiao Dong

doi:10.1016/j.bbrc.2019.04.137

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…In the study by You et al (2019)) and Kim et al (2004), the silk fibroin scaffolds prepared by salt leaching have an average pore size of more than 350 m. In contrast, the pores on the silk protein/136 ± 32 m RHLC scaffold. More importantly, the pores of the blend are highly interconnected, usually in the form of a network rather than an irregularity.…”

Section: Discussionmentioning

confidence: 99%

“…However, in order to apply to tissue engineering, the regeneration process is inevitable, which completely changes the performance of silk, especially its excellent mechanical properties. Although the technology of preparing porous silk scaffold materials such as gas phase forming method, salt immersion method, electrostatic spinning has been developed (Abhishek, Yeun, Eun, & Duong, 2013; Fini et al, 2005; Kim et al, 2004; Li et al, 2015; Lin, Jinying, & Mingqi, 2008; Qi, Mou, & Zhang, 2013; She, Zhang, & Jin, 2008; Wang, Zhang, Shao, & X C, 2005; You, Chang, & Zhang, 2019). However, in order to facilitate the application of tissue engineering, it is necessary to further improve the controllability and mechanical properties of pore distribution.…”

Section: Introductionmentioning

confidence: 99%

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Preparation recombination human‐like collagen/fibroin scaffold and promoting the cell compatibility with osteoblasts

Xiao

et al. 2020

J Biomedical Materials Res

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On this basis, a novel recombinant human‐like collagen (RHLC)/silk fibroin scaffold material with high porosity and controllable aperture was prepared. The compatibility of osteoblasts (OB) with the blends was tested in vitro. The morphology, adhesion and growth of scaffold cells were observed by scanning electron microscope and laser confocal microscope. Extensive measurements, including 3‐[4, 5‐dimethylthiazole‐2‐acyl]‐2, 5‐diphenyl tetrabrominate assays, intracellular total protein content, and alkaline phosphatase activity assays were performed after 7 days of culture. Survival and protein content increased in RHLC/fibroin stents. LSCM and SEM results confirmed that the cells grew better in the mixed scaffolds than in the pure silk scaffolds, and showed that the cells were easy to adhere and diffuse in the RHLC/silk scaffolds. RHLC/silk fibroin scaffolds are promising biomaterials for bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation recombination human‐like collagen/fibroin scaffold and promoting the cell compatibility with osteoblasts

Xiao

et al. 2020

J Biomedical Materials Res

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“…The CHSS alone was not utilized as a separate group, as CHSS transplantation alone without any functional modification into the lesion sites in rats and dogs has already been repeatedly validated to notably restrain deposition of chondroitin sulfate proteoglycans within the lesion center. 11,14,19,20 Furthermore, the scaffold alone showed very limited effect on promoting nerve regeneration. 21 Therefore, CHSS is a proper scaffold only to guide axonal regeneration and reduce glial scar formation.…”

Section: Methodsmentioning

confidence: 99%

Collagen/heparin sulfate scaffold combined with mesenchymal stem cells treatment for canines with spinal cord injury: A pilot feasibility study

Deng

Yang

Liang

et al. 2021

J Orthop Surg (Hong Kong)

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Background: Due to endogenous neuronal deficiency and glial scar formation, spinal cord injury (SCI) often leads to irreversible neurological loss. Accumulating evidence has shown that a suitable scaffold has important value for promoting nerve regeneration after SCI. Collagen/heparin sulfate scaffold (CHSS) has shown effect for guiding axonal regeneration and decreasing glial scar deposition after SCI. The current research aimed to evaluate the utility of the CHSSs adsorbed with mesenchymal stem cells (MSCs) on nerve regeneration, and functional recovery after acute complete SCI. Methods: CHSSs were prepared, and evaluated for biocompatibility. The CHSSs adsorbed with MSCs were transplanted into these canines with complete SCI. Results: We observed that MSCs had good biocompatibility with CHSSs. In complete transverse SCI models, the implantation of CHSS co-cultured with MSCs exhibited significant improvement in locomotion, motor evoked potential, magnetic resonance imaging, diffusion tensor imaging, and urodynamic parameters. Meanwhile, nerve fibers were markedly improved in the CHSS adsorbed with MSCs group. Moreover, we observed that the implantation of CHSS combined with MSCs modulated inflammatory cytokine levels. Conclusions: The results preliminarily demonstrated that the transplantation of MSCs on a CHSS could improve the recovery of motor function after SCI. Thus, implanting the MSCs-laden CHSS is a promising combinatorial therapy for treatment in acute SCI.

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“…You et al [119] utilized bone mesenchymal stem cells (BMSCs) seeded on a porous silk fibroin scaffold to enhance transplanted cells' survivability and promote nerve regeneration. The cell-seeded scaffold was noted to increase the markers for damaged axon regeneration and maintenance of the myelin structural and functional integrity, bridging the defected nerve with nerve fibers when applied to the transected spinal cord.…”

Section: Cell-seeded Scaffoldsmentioning

confidence: 99%

Novel Strategies for Spinal Cord Regeneration

Costăchescu

Niculescu

Dabija

et al. 2022

IJMS

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A spinal cord injury (SCI) is one of the most devastating lesions, as it can damage the continuity and conductivity of the central nervous system, resulting in complex pathophysiology. Encouraged by the advances in nanotechnology, stem cell biology, and materials science, researchers have proposed various interdisciplinary approaches for spinal cord regeneration. In this respect, the present review aims to explore the most recent developments in SCI treatment and spinal cord repair. Specifically, it briefly describes the characteristics of SCIs, followed by an extensive discussion on newly developed nanocarriers (e.g., metal-based, polymer-based, liposomes) for spinal cord delivery, relevant biomolecules (e.g., growth factors, exosomes) for SCI treatment, innovative cell therapies, and novel natural and synthetic biomaterial scaffolds for spinal cord regeneration.

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Cell-seeded porous silk fibroin scaffolds promotes axonal regeneration and myelination in spinal cord injury rats

Cited by 16 publications

References 30 publications

Preparation recombination human‐like collagen/fibroin scaffold and promoting the cell compatibility with osteoblasts

Preparation recombination human‐like collagen/fibroin scaffold and promoting the cell compatibility with osteoblasts

Collagen/heparin sulfate scaffold combined with mesenchymal stem cells treatment for canines with spinal cord injury: A pilot feasibility study

Novel Strategies for Spinal Cord Regeneration

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