Cell Secretion: Current Structural and Biochemical Insights

Trikha, Saurabh; Lee, Elizabeth C.; Jeremić, Aleksandar

doi:10.1100/tsw.2010.193

Cited by 9 publications

(4 citation statements)

References 140 publications

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“…For example, recently we described the important role of zymophagy (selective macroautophagy of activated zymogen granules) to prevent intracellular activation of zymogen granules in pancreatic acinar cells ( 8 ). These cells have a metabolism almost completely dedicated to protein synthesis to accomplish their function ( 44 ). Therefore, we can hypothesize that the TASCC strategy can allow the zymogen synthesis and at the same time the prophylactic basal zymophagy activity.…”

Section: Macroautophagy As Senescence Effectormentioning

confidence: 99%

Macroautophagy and the Oncogene-Induced Senescence

Grasso

Vaccaro

2014

Front. Endocrinol.

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The oncogene-induced senescence is emerging as a potent tumor suppressor mechanism and as a possible therapeutic target. Macroautophagy is intimately linked to the senescence condition setup, although its role has not been elucidated yet. Here, we discuss up-to-date concepts of senescence-related macroautophagy and evaluate the current trend of this growing research field, which has relevance in future perspectives toward therapeutic options against cancer.

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Section: Macroautophagy As Senescence Effectormentioning

confidence: 99%

Macroautophagy and the Oncogene-Induced Senescence

Grasso

Vaccaro

2014

Front. Endocrinol.

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“…Cell secretion is a fundamental and ubiquitous cell function involved in the regulation of various physiological processes, such as the digestion of food, neurotransmission, hormonal control of cell-and reproductive-cycles, and many other life processes. 272 Investigation of cell secretions is of great value in diagnosing, monitoring, assessing and treating related disease. Routine assays using ensemble biochemical measurements from a large cell population with a low temporal resolution are unable to resolve the fast kinetics of cell secretion and detect trace amounts of released molecules.…”

Section: Determining Dynamic Secretion Of Living Cellsmentioning

confidence: 99%

Graphene and its derivatives for cell biotechnology

Yang

Yao

Duan

2013

Analyst

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Every few years, a novel material with salient and often unique properties emerges and attracts both academic and industrial interest from the scientific community. The latest blockbuster is graphene, an increasingly important nanomaterial with atomically thin sheets of carbon, which has become a shining star and has shown great promise in the field of material science and nanotechnology. In recent years, it has changed from being the exclusive domain of physicists to the new passion of chemists and biologists. Graphene and its derivatives are now at the forefront of nearly every rapidly developing field of science and engineering, including biochemistry, biomedicine and certain cutting-edge interdisciplines that have intense popularity. The aim of this review is, firstly, to provide readers with a comprehensive, systematic and in-depth prospective of graphene's band structure and properties, and secondly, to concentrate on the recent progress in producing graphene-based nanomaterials, including mechanical exfoliation, chemical vapor deposition, plasma enhanced chemical vapor deposition, chemical reduction of graphene oxide, total organic synthesis, electrochemical synthesis and other fabrication strategies widely accepted by research scientists. At the same time, important definitions related to graphene are also introduced. The focus of this Tutorial Review is to emphasize the current situation and significance of using this new kind of two-dimensional material in the hot and emerging fields that are closely related to human life quality, for instance, cell biochemistry, bioimaging along with other frontier areas. Finally, the latest developments and possible impact that affect the heart of the whole scientific community have been discussed. In addition, the future trends along with potential challenges of this rapidly rising layered carbon have been pointed out in this paper.

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“…It has been suggested that vesicle swelling is involved in the exocytotic process [1,2,3]. In secretory vesicles, a specific set of ion channels expressed in the membrane has been proposed to contribute to the secretory granule swelling [4,5,6,7,8].…”

Section: Introductionmentioning

confidence: 99%

Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles

Centrone

Santo

Nicotera

et al. 2018

Cells

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Vesicle fusion is a fundamental cell biological process similar from yeasts to humans. For secretory vesicles, swelling is considered a step required for the expulsion of intravesicular content. Here this concept is revisited providing evidence that it may instead represent a general mechanism. We report the first example that non-secretory vesicles, committed to insert the Aquaporin-2 water channel into the plasma membrane, swell and this phenomenon is required for fusion to plasma membrane. Through an interdisciplinary approach, using atomic force microscope (AFM), a fluorescence-based assay of vesicle volume changes and NMR spectroscopy to measure water self-diffusion coefficient, we provide evidence that Gi protein modulation of potassium channel TASK-2 localized in AQP2 vesicles, is required for vesicle swelling. Estimated intravesicular K+ concentration in AQP2 vesicles, as measured by inductively coupled plasma mass spectrometry, was 5.3 mM, demonstrating the existence of an inwardly K+ chemical gradient likely generating an osmotic gradient causing vesicle swelling upon TASK-2 gating. Of note, abrogation of K+ gradient significantly impaired fusion between vesicles and plasma membrane. We conclude that vesicle swelling is a potentially important prerequisite for vesicle fusion to the plasma membrane and may be required also for other non-secretory vesicles, depicting a general mechanism for vesicle fusion.

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Cell Secretion: Current Structural and Biochemical Insights

Cited by 9 publications

References 140 publications

Macroautophagy and the Oncogene-Induced Senescence

Macroautophagy and the Oncogene-Induced Senescence

Graphene and its derivatives for cell biotechnology

Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles

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