Cell–scaffold mechanical interplay within engineered tissue

Dado, Dekel; Levenberg, Shulamit

doi:10.1016/j.semcdb.2009.02.001

Cited by 139 publications

(103 citation statements)

References 72 publications

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“…VEGF is responsible for initiating vessel formation and inducing molecular and cellular events leading to network stabilization and maturation (39). VEGF inhibition in a coculture of ECs and fibroblasts was predicted to damage the network only whereas the level of forces could be maintained by the fibroblasts (19,40,41). However, the presented results suggest that the force measured during network assembly is applied by both cell types, which provide a synergistic effect on overall force when collaborating to form elongated tubes.…”

Section: Discussionmentioning

confidence: 77%

“…Both external tensile forces and cell-induced forces act on the cell cytoskeleton and are mostly transmitted to the cell through the actomyosin pathway, adhesion sites, and cell stress fibers (18). In addition, several studies have reported measurement of cell-induced forces on their substrates (19) but have hardly focused on identifying a correlation between the level of cell-induced force and tubular network organization.…”

mentioning

confidence: 99%

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Morphogenesis of 3D vascular networks is regulated by tensile forces

Rosenfeld

Landau

Shandalov

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Understanding the forces controlling vascular network properties and morphology can enhance in vitro tissue vascularization and graft integration prospects. This work assessed the effect of uniaxial cell-induced and externally applied tensile forces on the morphology of vascular networks formed within fibroblast and endothelial cell-embedded 3D polymeric constructs. Force intensity correlated with network quality, as verified by inhibition of force and of angiogenesis-related regulators. Tensile forces during vessel formation resulted in parallel vessel orientation under static stretching and diagonal orientation under cyclic stretching, supported by angiogenic factors secreted in response to each stretch protocol. Implantation of scaffolds bearing network orientations matching those of host abdominal muscle tissue improved graft integration and the mechanical properties of the implantation site, a critical factor in repair of defects in this area. This study demonstrates the regulatory role of forces in angiogenesis and their capacities in vessel structure manipulation, which can be exploited to improve scaffolds for tissue repair.

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Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

Morphogenesis of 3D vascular networks is regulated by tensile forces

Rosenfeld

Landau

Shandalov

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

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“…In addition, cells in a wound site will exert contractile forces on the scaffold material. 90,91 Therefore, the response of scaffold architecture to deformation should also be considered carefully.…”

Section: Limitations and Future Directions Tissue Engineering Environmentioning

confidence: 99%

Quantitative architectural description of tissue engineering scaffolds

Ashworth

Best

Cameron

2014

Materials Technology

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Arguably one of the most specialised subtopics in porous materials research is that of tissue engineering scaffolds. The porous architecture of these scaffolds is a key variable in determining biological response. However, techniques for characterising these materials tend to vary widely in the literature. There is a need for a set of transferable and effective methods for architectural characterisation. In this review, four key areas of importance are addressed. First, the definition and interpretation of pore size are considered in relation to fluid transport properties, by analogy with filtration research. Second, the definition of interconnectivity is discussed using insight obtained from cement and concrete research. Third, the issue of data scalability is addressed by consideration of percolation theory, as implemented for the study of geological materials. Finally, emerging techniques such as confocal and multiphoton microscopy are discussed. These methods allow the three-dimensional observation of pore strut arrangement, as well holding great potential for understanding changes in pore architecture under dynamic conditions.

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“…Several topical reviews covering microfluidic tissue engineering have been published [190][191][192][193]. The most recent review by Huang et al [194] summarises the developments in the fabrication of microfluidic hydrogels for using in tissue engineering.…”

Section: Tissue Engineering In Microbioreactorsmentioning

confidence: 99%