Cell responses to metallic nanostructure arrays with complex geometries

Jahed, Zeinab; Molladavoodi, Sara; Seo, Brandon B.; Gorbet, Maud; Tsui, Ting Y.; Mofrad, Mohammad R. K.

doi:10.1016/j.biomaterials.2014.07.022

Cited by 41 publications

(35 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, long NW have been associated with decreased cell viability through detrimental interactions with the cell nucleus . Other studies report that increasing NW height impairs cell function and division, and induces DNA damage . Thus, we first examined the influence of NW height on cell viability and proliferation (Figures S6 and S7, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, cell membrane curvature induced by interaction with the NW may trigger endocytic pathways that enhance DNA uptake. The accommodation of the NW topography by the cell membrane, combined with the filopodia protrusions contribute to the mechanical strength of the cell–NW interface, enhancing cellular adhesion . NW were largely embedded within the membrane without apparent membrane rupture (Figure d, red arrow).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Maximizing Transfection Efficiency of Vertically Aligned Silicon Nanowire Arrays

Elnathan

Delalat

Brodoceanu

et al. 2015

Adv Funct Materials

111

119

View full text Add to dashboard Cite

Vertically aligned silicon nanowire (VA‐SiNW) arrays are emerging as a powerful new tool for gene delivery by means of mechanical transfection. In order to utilize this tool efficiently, uncertainties around the required design parameters need to be removed. Here, a combination of nanosphere lithography and templated metal‐assisted wet chemical etching is used to fabricate VA‐SiNW arrays with a range of diameters, heights, and densities. This fabrication strategy allows identification of critical parameters of surface topography and consequently the design of SiNW arrays that deliver plasmid with high transfection efficiency into a diverse range of human cells whilst maintaining high cell viability. These results illuminate the cell‐materials interactions that mediate VA‐SiNW transfection and have the potential to transform gene therapy and underpin future treatment modalities.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Maximizing Transfection Efficiency of Vertically Aligned Silicon Nanowire Arrays

Elnathan

Delalat

Brodoceanu

et al. 2015

Adv Funct Materials

111

119

View full text Add to dashboard Cite

show abstract

“…Simultaneously, nanopatterning materials can fundamentally alter their mechanical properties (indeed this is one approach to creating mechanical metamaterials) . Hence, our perception of materials being hard or stiff is often inaccurate at the nanoscopic level, as illustrated by the many examples of cells deforming nanostructures made from macroscopically stiff materials . Even diamond nanoneedles undergo large elastic deformations at the nanoscale .…”

Section: Discussionmentioning

confidence: 99%

“…[14] Hence, our perception of materials being hard or stiff is often inaccurate at the nanoscopic level, as illustrated by the many examples of cells deforming nanostructures made from macroscopically stiff materials. [69,95,128,131,150,224,476] Even diamond nanoneedles undergo large elastic deformations at the nanoscale. [477] While stiffnessrelated effects have been studied extensively, [478] and shown to Adv.…”

Section: Open Questionsmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

View full text Add to dashboard Cite

Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

show abstract

“…3 Furthermore, silicon electrodes with vertical nanopillar arrays were successfully incorporated into a variety of high-performance energy devices, such as photovoltaics, 4 photo-electrochemical cells, 5 and lithium-ion batteries, 6 benefiting from minimized light reflection and tolerance towards lithiationinduced volume expansion. [7][8][9][10] In particular, vertical silicon nanowire-based biomolecular delivery 11,12 and stem cell fate control 13,14 have been intensively studied. [7][8][9][10] In particular, vertical silicon nanowire-based biomolecular delivery 11,12 and stem cell fate control 13,14 have been intensively studied.…”

Section: Introductionmentioning

confidence: 99%

Polyelectrolyte multilayer-assisted fabrication of non-periodic silicon nanocolumn substrates for cellular interface applications

Lee

Kim

et al. 2015

Nanoscale

View full text Add to dashboard Cite

Recent advances in nanostructure-based biotechnology have resulted in a growing demand for vertical nanostructure substrates with elaborate control over the nanoscale geometry and a high-throughput preparation. In this work, we report the fabrication of non-periodic vertical silicon nanocolumn substrates via polyelectrolyte multilayer-enabled randomized nanosphere lithography. Owing to layer-by-layer deposited polyelectrolyte adhesives, uniformly-separated polystyrene nanospheres were securely attached on large silicon substrates and utilized as masks for the subsequent metal-assisted silicon etching in solution. Consequently, non-periodic vertical silicon nanocolumn arrays were successfully fabricated on a wafer scale, while each nanocolumn geometric factor, such as the diameter, height, density, and spatial patterning, could be fully controlled in an independent manner. Finally, we demonstrate that our vertical silicon nanocolumn substrates support viable cell culture with minimal cell penetration and unhindered cell motility due to the blunt nanocolumn morphology. These results suggest that vertical silicon nanocolumn substrates may serve as a useful cellular interface platform for performing a statistically meaningful number of cellular experiments in the fields of biomolecular delivery, stem cell research, etc.

show abstract

Cell responses to metallic nanostructure arrays with complex geometries

Cited by 41 publications

References 26 publications

Maximizing Transfection Efficiency of Vertically Aligned Silicon Nanowire Arrays

Maximizing Transfection Efficiency of Vertically Aligned Silicon Nanowire Arrays

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Polyelectrolyte multilayer-assisted fabrication of non-periodic silicon nanocolumn substrates for cellular interface applications

Contact Info

Product

Resources

About