Cell Respiration of Rat Cardiomyocytes and Soleus Muscle Fibers under Ultra-Short-Term Antiorthostatic Suspension

Biryukov, Nikolay S.; Veselova, Oksana; Ларина, И. М.

doi:10.21103/article4_3_er1

Cited by 3 publications

(3 citation statements)

References 53 publications

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“…This transient accumulation could lead to the activation of STAT3 and an increase in the rate of cellular respiration (Figure 5). Moreover, we observed the dissociation of alpha-actinin-1 and an increase in the rate of cellular respiration in the heart muscle when simulating microgravity in rats [22,39]. Furthermore, the content of alpha-actinin decreased with the decreasing content of actin, which we also observed in the present study.…”

Section: Discussionsupporting

confidence: 89%

Mitochondrial Respiration in Drosophila Ovaries after a Full Cycle of Oogenesis under Simulated Microgravity

Ogneva,

Usik

2021

CIMB

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Studies of the function of the female reproductive system in zero gravity are urgent for the future exploration of deep space. Female reproductive cells, oocytes, are rich in mitochondria, which allow oocytes to produce embryos. The rate of cellular respiration was determined to assess the functional state of the mitochondrial apparatus in Drosophila melanogaster ovaries in which the full cycle of oogenesis took place under simulated microgravity. Since cellular respiration depends on the state of the cytoskeleton, the contents of the main cytoskeletal proteins were determined by Western blotting. To modulate the structure of the cytoskeleton, essential phospholipids were administered per os at a dosage of 500 mg/kg in medium. The results of this study show that after a full cycle of oogenesis under simulated microgravity, the rate of cellular respiration in the fruit fly ovaries increases, apparently due to complex II of the respiratory chain. At the same time, we did not find any changes in the area of oocytes or in the content of proteins in the respiratory chain. However, changes were found in the relative contents of proteins of the actin cytoskeleton. There were no changes of essential phospholipids and no increase in the rate of cellular respiration of the ovaries after exposure to simulated microgravity. However, in the control, the administration of essential phospholipids led to a decrease in the efficiency of oxygen consumption in the flies’ ovaries due to complexes IV–V.

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Section: Discussionsupporting

confidence: 89%

Mitochondrial Respiration in Drosophila Ovaries after a Full Cycle of Oogenesis under Simulated Microgravity

Ogneva,

Usik

2021

CIMB

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“…The motor activity of sperm is determined by the state of the microtubule cytoskeleton, which provides motility, and its energy supply. Previous studies showed that a decrease in the mechanical load on skeletal muscle cells leads to a decrease in the efficiency of cell respiration [ 12 , 13 , 14 , 15 , 16 ] and an increase in the volume mechanical load, particularly on cardiomyocytes, to an increase in the rate of energy production [ 14 ]. Therefore, we hypothesized that the observed changes in motility may be associated with changes in cell respiration.…”

Section: Discussionmentioning

confidence: 99%

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Biryukov

Zhdankina

2020

IJMS

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For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility.

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“…Currently, there are data demonstrating that alpha-actinin-4 dissociates from the cortical cytoskeleton into the cytoplasm in the soleus muscle cells at the earliest stages (6 hours) of hindlimb suspension [ 17 ]. Furthermore, subsequently, there are alterations in the mRNA content of the alpha-actinin-4 and decrease in gene expression of cytochrome c (one of the basic in cell respiration) [ 18 ]. However, we can suggest that such dynamics may be typical not only for the alpha-actinin-4, but also for other actin-binding proteins like as proteins of the Arp2/3 complexes, tropomodulin, supervillin, L-plastin.…”

Section: Introductionmentioning

confidence: 99%

Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse

Biryukov

2016

PLoS ONE

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The aim of this study was to prevent the cortical cytoskeleton reorganization of rat soleus muscle fibers under short-term gravitational disuse. Once a day, we injected the right soleus muscle with 0.5 ml lecithin at a concentration of 200 mg/ml and the left soleus muscle with a diluted solution in an equal volume for 3 days prior to the experiment. To simulate microgravity conditions in rats, an anti-orthostatic suspension was used according to the Ilyin-Novikov method modified by Morey-Holton et al. for 6 hours. The following groups of soleus muscle tissues were examined: «C», «C+L», «HS», and «HS+L». The transversal stiffness of rat soleus muscle fibers after 6 hours of suspension did not differ from that of the control group for the corresponding legs; there were no differences between the groups without lecithin «C» and «HS» or between the groups with lecithin «C+L» and «HS+L». However, lecithin treatment for three days resulted in an increase in cell stiffness; in the «C+L» group, cell stiffness was significantly higher by 22.7% (p < 0.05) compared with that of group «C». The mRNA content of genes encoding beta- and gamma-actin and beta-tubulin did not significantly differ before and after suspension in the corresponding groups. However, there was a significant increase in the mRNA content of these genes after lecithin treatment: the beta-actin and gamma-actin mRNA content in group «C+L» increased by 200% compared with that of group «C», and beta-tubulin increased by 100% (as well as the mRNA content of tubulin-binding proteins Ckap5, Tcp1, Cct5 and Cct7). In addition, desmin mRNA content remained unchanged in all of the experimental groups. As a result of the lecithin injections, there was a redistribution of the mRNA content of genes encoding actin monomer- and filament-binding proteins in the direction of increasing actin polymerization and filament stability; the mRNA content of Arpc3 and Lcp1 increased by 3- and 5-fold, respectively, but the levels of Tmod1 and Svil decreased by 2- and 5-fold, respectively. However, gravitational disuse did not result in changes in the mRNA content of Arpc3, Tmod1, Svil or Lcp1. Anti-orthostatic suspension for 6 hours resulted in a decrease in the mRNA content of alpha-actinin-4 (Actn4) and alpha-actinin-1 (Actn1) in group «HS» compared with that of group «C» by 25% and 30%, respectively, as well as a decrease and increase in the ACTN4 protein content in the membrane and cytoplasmic fractions, respectively. Lecithin injection resulted in an increase in the Actn1 and Actn4 mRNA content in group «C+L» by 1.5-fold and more than 2-fold, respectively, compared with the levels in group «C». Moreover, in group «HS+L», the mRNA content did not change in these genes compared with the levels in group «C+L», and the ACTN4 protein content in the membrane and cytoplasmic fractions also remained unchanged. Thus, lecithin prevented the reduction of Actn1 and Actn4 mRNA and the migration of ACTN4 from the cortical cytoskeleton to the cytoplasm.

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Cell Respiration of Rat Cardiomyocytes and Soleus Muscle Fibers under Ultra-Short-Term Antiorthostatic Suspension

Cited by 3 publications

References 53 publications

Mitochondrial Respiration in Drosophila Ovaries after a Full Cycle of Oogenesis under Simulated Microgravity

Mitochondrial Respiration in Drosophila Ovaries after a Full Cycle of Oogenesis under Simulated Microgravity

Sperm Motility of Mice under Simulated Microgravity and Hypergravity

Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse

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