Cell renewal of the rat corneal epithelium

Håskjold, Erling; Refsum, Sigvald; Bjerknes, Rolf

doi:10.1111/j.1755-3768.1988.tb04376.x

Cited by 25 publications

(4 citation statements)

References 6 publications

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“…In general,corneal neoplasms are mostly reported to involve the limbus (Pizzarello & Jakobiec 1978). Transformed epithelial cells therefore might be replaced by limbal cells before tumor formation occur (Haaskjold 1988).…”

Section: Discussionmentioning

confidence: 99%

UVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium

Estil

Olsen

Huitfeldt

et al. 1997

Acta Ophthalmologica Scandinavica

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ABSTRACT. The induction of DNA photoproducts in rat corneal epithelium was studied after in vivo exposure to different doses of ultraviolet B light at 297 nm. Affinity-purified antibodies with a major specificity against UV-induced (6-4) photoproducts were used. The results indicate a dose dependent formation of (6-4) photoproducts. Even a minimal erythema dose (25 mJ/cm2) produced (6-4) photoproducts, demonstrating that DNA damage occurs in corneal tissue following exposure to biologically relevant doses of UVB light.

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Section: Discussionmentioning

confidence: 99%

UVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium

Estil

Olsen

Huitfeldt

et al. 1997

Acta Ophthalmologica Scandinavica

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“…In addition, the LI and the MR were registered for each vision field in both the corneal and the conjunctival epithelium of each eye. As the number of vision fields across the diameter of the cornea varies from specimen to specimen, we used a previously described mathematical method to correlate corresponding corneal areas from different eyes (Haaskjold et al 1988). By this method corneal sections were normalized to 32 'ideal' microscopical vision fields.…”

Section: Methodsmentioning

confidence: 99%

Cell kinetics of conjunctival and corneal epithelium during regeneration of different‐sized corneal epithelial defects

Sandvig

Haaskjold

Bjerknes

et al. 1994

Acta Ophthalmologica

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Rats with small (diam. 1.7 mm), medium sized (diam. 3.5 mm) or large (diam. 5.5 mm) corneal epithelial erosions in one eye were killed 1, 2 or 4 days after the injury. The proliferative response was evaluated by measuring the labelling index and the mitotic rate in the corneal epithelium and in the adjacent conjunctiva. The small erosions triggered a proliferative response in the cornea only with the maximum response occurring midperipherally. The medium sized erosions induced a higher and more extensive response in the cornea and also a slight increase of the labelling index in the limbal area. The large erosions induced an even more pronounced response in the peripheral cornea and an increase both of the labelling index and the mitotic rate well beyond the limbal part of the conjunctiva. It is concluded that the magnitude and the extent both of the conjunctival and the corneal regenerative response to a corneal abrasion is correlated to the size of the corneal defect. Temporary reduction in the conjunctival epithelial cell number shows that both cells in the limbal and the extralimbal conjunctiva migrate centripetally during healing of large corneal wounds. It is suggested that the stem cell theory should be modified. The limbal area is probably an area in which conjunctival epithelial cells or conjunctiva-derivated cells transform or differentiate to corneal epithelial cells.

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“…Известно, что в структурах конъюнктивальной оболочки глаза и в эпителии роговицы имеются обновляющиеся клеточные популяции, в которых процессы пролиферации и апоптоза являются строго уравновешенными. При нарушении баланса между этими процессами в результате действия различных повреждающих факторов могут возникать патологические изменения передней поверхности глаза [23]. Нами был разработан способ моделирования гипоксического поражения тканей глаза с активацией апоптоза, заключающийся в воздействии физического фактора на экспериментальное животное.…”

Section: обсуждение результатовunclassified

The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo

Рябцева

Akberova²,

Али-заде

et al. 2021

Oftalʹmologiâ

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Purpose: to evaluate the effects of acute hypoxic hypoxia on eye tissue cells in adult rats and determining the effectiveness of 0.007 % solution of para-aminobenzoic acid (PABA) for apoptotic damage to the tissues of the ocular surface in rats.Materials and methods. In the experiment, 27 male Wistar rats (54 eyes) were used, divided into 5 groups. Group I — intact control, Group II — hypoxic control after 1 hour, Group III — hypoxic control after 3 hours, Group IV — with the injection of the drug 1 hour after hypoxia, Group V — injection of the drug 24 hours before hypoxia. The identification of apoptotic cells in the tissues of the eye was performed by the TUNEL method on frozen sections of the eye stained with Hoechst 33342 nuclear fluorescent dye. The localization and fluorescence intensity of the damaged cells was analyzed under a fluorescence microscope using the Image J. computer program.Results. Under the conditions of simulated acute hypoxic hypoxia, apoptotic lesions of the conjunctiva, corneal epithelium, choroid, and photoreceptor layer of the retina were observed. In group II, the number of damaged cells in the conjunctiva was 67 %, and in group III it was 120 % more compared to group I — the norm (p < 0.05). With the injection of PABA before hypoxia, there were no significant differences compared with the norm (group I) in the state of the affected cells in the conjunctiva. In the group of animals (group III) with the injection of PABA after hypoxia after 1 h, the number of damaged cells does not statistically differ from group II (hypoxic control after 1 h), but significantly lower than in group III (hypoxic control after 3 hours). The same pattern is observed in the corneal epithelium.Conclusion. Para-aminobenzoic acid (0.007 %) has a prophylactic and therapeutic effect, preventing and stabilizing the development of apoptosis of conjunctival cells and anterior corneal epithelium induced in experimental acute hypoxic hypoxia.

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Cell renewal of the rat corneal epithelium

Cited by 25 publications

References 6 publications

UVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium

UVB‐induced formation of (6–4) photoproducts in the rat corneal epithelium

Cell kinetics of conjunctival and corneal epithelium during regeneration of different‐sized corneal epithelial defects

The Protective Effect of Para-Aminobenzoic Acid in HypoxiaInduced Apoptosis of Conjunctiva and Cornea Cells in vivo

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