Cell recognition in plants: Determinants of the stigma surface and their pollen interactions

Knox, R. B.; Clarke, Adrienne E.; Harrison, S.; Smith, Patricia M.; Marchalonis, John J.

doi:10.1073/pnas.73.8.2788

Cited by 123 publications

(55 citation statements)

References 17 publications

(23 reference statements)

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“…Fewer E. nitens pollen tubes were also counted in the E. globulus style. However, this was consistent with the lower in vitro germination of the E. nitens pollen utilised and there is no evidence of stigmatic inhibition of pollen germination or initial penetration of the style (Knox et al 1976;Stettler et al 1980). Two distinct, although not mutually exclusive, groups of mechanisms (de Nettancourt 1984) have been proposed to account for interspecific incompatibility (sensu de Nettancourt 1977, p. 141).…”

Section: Discussionmentioning

confidence: 50%

Unilateral Cross-Incompatibility in Eucalyptus: the Case of Hybridisation Between E. globulus and E. nitens

Gore¹,

Potts²,

Volker³

et al. 1990

Aust. J. Bot.

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The growth of E. globulus and E. nitens pollen tubes in styles of E. globulus was examined in order to elucidate the site of the unilateral barrier to hybridisation. Pollen tubes of E. nitens failed to grow the full length of the larger E. globulus style. E. globulus pollen tubes grew an average of 1.4 mm per day for the first 4 days, compared with 0.8 mm per day for pollen tubes of E. nitens. From days 4 to 14, the growth of E. nitens pollen tubes slowed to an average of 0.2 mm per day and virtually no growth occurred after day 14. In contrast, E. globulus pollen tubes grew through the style and into the ovary between days 5 and 14. By day 28, at about the time of style abscission, E. nitens tubes had grown only 6 mm, well short of the full length of the E. globulus style (9-10 mm). A similar difference in growth was obtained in vitro where E. nitens pollen tubes were significantly shorter than those of E. globulus. A comparison also including E. ovata, E. urnigera and E. gunnii indicated a significant correlation between style length and in vitro pollen tube length. It is argued that the unilateral cross-incompatibility between E. globulus and E. nitens is due to a structural barrier arising from an inherent limit to pollen tube growth which is associated with pistil size.

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Section: Discussionmentioning

confidence: 50%

Unilateral Cross-Incompatibility in Eucalyptus: the Case of Hybridisation Between E. globulus and E. nitens

Gore¹,

Potts²,

Volker³

et al. 1990

Aust. J. Bot.

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“…Recently, it has been revealed that carbohydrates or glycoproteins serve as determinants of the specificity of various systems such as sexual mating of yeast (21), species-specific aggregation of sponge cells (17), and pollination in plants (11). The results in this paper indicated that the spore-agglutinating factor is a glycoprotein, of which the carbohydrate moiety has a role in its agglutinating activity (Table I).…”

Section: Resultsmentioning

confidence: 60%

Isolation and Characterization of Factors in Sweet Potato Root Which Agglutinate Germinated Spores of Ceratocystis fimbriata, Black Rot Fungus

Kojima¹,

Uritani²

1978

Plant Physiol.

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A factor which agglutinates the germinated spores of Ceratocystis fIunbriata was isolated from the sweet potato root. The factor is a glycoprotein with a molecular weight of 1.6 x 106 daltons and required divalent cations such as Ca2+, Mn2+, Ni2+, and Mg2 for activity. The activity of the factor was pH-dependent. The factor also agglutinated rabbit erythrocytes and is classified as a phytohemagglutinin or lectin. The factor agglutinated germinated spores of seven strains of C. fimbrista to almost the same degree. The factor showed differentimIl agglutinating activity toward the strains in the presence of unidentified low molecular weight factor(s) in the sweet potato root. These results support our earlier suggestion that the spore-agglutinating factors in host plants function as the determinants of specificity in some host-parasite interactions.It has been shown that many plants contain phytohemagglutinins or lectins (3,4,6,18). Also, much information has been accumulated on their nature and mode of action on animal cells (3,4,6,7,16,19). However, their role in the plant is unclear.Recently, some investigators (1,2,10,12, 23) implied that lectins are involved in the determination of plant host-parasite specificity. Bohlool and Schmidt (2)

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“…Golynskaya et al (7) found hemagglutinins in Primula stigmata which also effected pollen tube growth. Pretreatment of Gladiolus stigmata with Con A prevented compatible pollen tubes from penetrating (10). There is also considerable evidence that the specificity of host-symbiont interactions is determined by lectin binding (1,3,4,8,15,21).…”

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confidence: 99%

Plant Protoplast Agglutination by Lectins

Larkin¹

1978

Plant Physiol.

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Coicanavain A, soybean (Glyce max L.) ectin, castor bea (Rkhns communis L.) lectn, and peanut (Machis hypogaea L.) lectin were able to agglutinate protoplasts prepared from a variety of plant species. The seven other lectins tried were unable to agglutinate those protoplasts tested. Protoplasts prepared from 11 species were used. The lectins examined were not able to differentiate among protoplasts of different species.Recently there has been some interest in the effects of plant lectins on plant cell systems. Phaseolus vulgaris lectin was shown to have a mitogenic effect on tomato callus cells (13) and barley and pea root tip cells (17). Soybean lectin was mitogenic for soybean callus cells (9). PHA2 and Con A both stimulate Lilium pollen germination (18). Golynskaya et al. (7) found hemagglutinins in Primula stigmata which also effected pollen tube growth. Pretreatment of Gladiolus stigmata with Con A prevented compatible pollen tubes from penetrating (10). There is also considerable evidence that the specificity of host-symbiont interactions is determined by lectin binding (1,3,4,8,15,21). Gamborg and Miller (5) suggested that lectins may be useful for protoplast aggregation which in turn would facilitate fusion. Since then Glimelius et al. (6) reported the agglutination of carrot culture cell protoplasts by Con A. They observed inhibition of the agglutination by methyl-a-D-glucoside and a great reduction of agglutination using glutaraldehyde-fixed protoplasts. Low temperature (4 C) limited agglutination and higher cell densities favored higher agglutination percentages. Burgess and Linstead (2) confirmed the ability of Con A to interact with protoplast surfaces. Using Con A conjugated with colloidal gold they demonstrated both specific binding (inhibited by 0.1 M a-methyl mannoside) to plasmalemma of Vitis vinifera culture and tobacco leaf protoplasts, and also nonspecific binding to amorphous material near the plasmalemma. The ability of plant lectins to interact with plasmalemma may be of significance to their physiological role. Ruesink (16) and Williamson et aL (19,20) (200 g, uncooked) were milled, defatted with 500 ml of petroleum ether, and air-dried. A suspension in 0.9% (w/v) NaCl was stirred for 3 hr and the insoluble matter removed twice by centrifugation. (NH4)SO4 was added to 60% (w/v) saturation and the centrifuged pellet redissolved in 110 ml of 0.9%/v (w/v) NaCl and dialyzed against 0.9%o (w/v) NaCl for 24 hr with one change of saline. One sample was also dialyzed against OSI (pH 7.2) for 24 hr.Vicia faba beans (40 g) were milled, defatted with 300 ml of petroleum ether, and dried in air. The flour was suspended and stirred for I hr in 200 ml of 0.9% (w/v) NaCl. The centrifuged sediment was discarded. The supernatant was fractionated by (NH4)SO4 precipitation into the 0 to 48%, 48 to 65%, 65 to 80%o and 80 to 100%o (w/v)

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Cell recognition in plants: Determinants of the stigma surface and their pollen interactions

Cited by 123 publications

References 17 publications

Unilateral Cross-Incompatibility in Eucalyptus: the Case of Hybridisation Between E. globulus and E. nitens

Unilateral Cross-Incompatibility in Eucalyptus: the Case of Hybridisation Between E. globulus and E. nitens

Isolation and Characterization of Factors in Sweet Potato Root Which Agglutinate Germinated Spores of Ceratocystis fimbriata, Black Rot Fungus

Plant Protoplast Agglutination by Lectins

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