Cell Proteomic Footprinting: Advances in the Quality of Cellular and Cell-Derived Cancer Vaccines

Abstract

In omics sciences, many compounds are measured simultaneously in a sample in a single run. Such analytical performance opens up prospects for improving cellular cancer vaccines and other cell-based immunotherapeutics. This article provides an overview of proteomics technology, known as cell proteomic footprinting. The molecular phenotype of cells is highly variable, and their antigenic profile is affected by many factors, including cell isolation from the tissue, cell cultivation conditions, and storage procedures. This makes the therapeutic properties of cells, including those used in vaccines, unpredictable. Cell proteomic footprinting makes it possible to obtain controlled cell products. Namely, this technology facilitates the cell authentication and quality control of cells regarding their molecular phenotype, which is directly connected with the antigenic properties of cell products. Protocols for cell proteomic footprinting with their crucial moments, footprint processing, and recommendations for the implementation of this technology are described in this paper. The provided footprints in this paper and program source code for their processing contribute to the fast implementation of this technology in the development and manufacturing of cell-based immunotherapeutics.

Pyridinium-Based Strategy for a Bioorthogonal Conjugation-Assisted Purification Method for Profiling Cell Surface Proteome

“…9 These studies have significant implications for targeted therapy, immunotherapy, and vaccine development. 10 Therefore, activity-based protein profiling (ABPP) is emerging as a reliable chemoproteomic technology associated with an analytical and synthetic toolbox for chemically profiling the proteome. Unfortunately, selectively enriching the CSPs remains technically challenging because of their low abundance and the absence of specific capture motifs.…”

“…Surface proteome studies are crucial for understanding the diversity and function of cell surface proteins. − Recent studies have utilized advanced techniques to identify and quantify cell surface proteins in different cell types, including immune and cancer cells . These studies have significant implications for targeted therapy, immunotherapy, and vaccine development . Therefore, activity-based protein profiling (ABPP) is emerging as a reliable chemoproteomic technology associated with an analytical and synthetic toolbox for chemically profiling the proteome.…”