Cell proliferation, viability, and in vitro differentiation of equine mesenchymal stem cells seeded on bacterial cellulose hydrogel scaffolds

Favi, Pelagie; Benson, Robert D.; Neilsen, Nancy R.; Hammonds, Ryan L.; Bates, Cassandra C; Stephens, C.P.; Dhar, Madhu

doi:10.1016/j.msec.2012.12.100

Cited by 101 publications

(71 citation statements)

References 51 publications

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“…The surfaces of BNC scaffolds were uneven with a porous structure, which allowed the cells to penetrate into the biopolymer structure. Therefore, pure BNC scaffolds slightly enhanced the differentiation process [14,58]. What is more, some findings proved that the negatively charged surfaces of scaffolds could promote cell dedifferentiation [64].…”

Section: Relative Gene Expressionmentioning

confidence: 91%

“…The effect of the scaffold on the viability of ATDC5 cells is presented in Figure 8. As the biocompatibility of pure BNC produced by K. xylinus E25 is well known [58] the significant differences in the viability of cells cultivated on the BNC control and nearly all BNC/κ-Car composites were promising. After 21 days of growth on various BNC/κ-Car composites ATDC5 cells exhibited the increased cell viability in comparison to the BNC control sample.…”

Section: The Viability Of Atdc5 Cellsmentioning

confidence: 99%

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Novel Bionanocellulose/κ-Carrageenan Composites for Tissue Engineering

et al. 2018

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Featured Application: Bionanocellulose/κ-carrageenan composites developed within this study meet the specific tissue engineering requirements, including high tensile and compression strength, water holding capacity, and water retention ratio, suitable swelling properties as well as the positive effect on cell differentiation. As the key properties of these composites may be easily modified during their fabrication, the established procedure may lead to the production of customized scaffolds.Abstract: In this work, novel bacterial cellulose/κ-carrageenan (BNC/κ-Car) composites, being potential scaffolds for tissue engineering (TE), and outperforming the two polymers when used as scaffolds separately, were for the first time obtained using an in situ method, based on the stationary culture of bacteria Komagateibacter xylinus E25. The composites were compared with native BNC in terms of the morphology of fibers, chemical composition, crystallinity, tensile and compression strength, water holding capacity, water retention ratio and swelling properties. Murine chondrogenic ATDC5 cells were applied to assess the utility of the BNC/κ-Car composites as potential scaffolds. The impact of the composites on the cells viability, chondrogenic differentiation, and expression patterns of Col1α1, Col2α1, Runx2, and Sox9, which are indicative of ATDC5 chondrogenic differentiation, was determined. None of the composites obtained in this study caused the chondrocyte hypertrophy. All of them supported the differentiation of ATDC5 cells to more chondrogenic phenotype.

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Section: Relative Gene Expressionmentioning

confidence: 91%

Section: The Viability Of Atdc5 Cellsmentioning

confidence: 99%

Novel Bionanocellulose/κ-Carrageenan Composites for Tissue Engineering

et al. 2018

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“…BC has been proposed for application in tissue engineering as a scaffold for cartilage [66,67], bone repair [68][69][70][71], vascular grafts [64,[72][73][74][75] and neural repair [75][76][77][78], or as a barrier membrane for guided bone regeneration [57,58,60,70,71,79]. In addition several composite materials have been developed with the purpose of rendering BC bioactive such as hydroxyapatite (HA) [79][80][81][82][83][84][85], collagen [70,[86][87][88], gelatin [85,89], peptides [71,90] and some polymers such as chitosan [35], silk [90][91][92][93], polyhydroxybutyrate [94,95] and poly(2-hydroxyethyl methacrylate) [96].…”

Section: Scaffoldmentioning

confidence: 99%

“…Results have shown that the combination of a BC hydrogel and equine MSCs are promising for musculoskeletal tissue engineering applications, allowing osteogenic and chondrogenic differentiation of equine MSCs [67].…”

Section: Scaffoldmentioning

confidence: 99%

Bacterial Cellulose

Barud

Gutierrez²,

Lustri

et al. 2016

Biomaterials From Nature for Advanced Devices and Therapies

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“…Our research group have developed and characterized the in vitro properties of a polymer-ceramic composite, using bacterial cellulose (BC) and physiological calcium-deficient hydroxyapatite (CdHA), an osteoconductive and bioresorbable mineral, for bone tissue regeneration [9,10]. Furthermore, additional work from our group has shown that BC polymer scaffolds promote mesenchymal stem cell adhesion, proliferation and differentiation into osteocytes in vitro [11].…”

Section: Introductionmentioning

confidence: 99%

Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Biodegradable Calcium-deficient Hydroxyapatite Tubular Bacterial Cellulose Composites

et al. 2014

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Advanced biomaterials that mimic the structure and function of native tissues and permit stem cells to adhere and differentiate is of paramount importance in the development of stem cell therapies for bone defects. Successful bone repair approaches may include an osteoconductive scaffold that permits excellent cell adhesion and proliferation, and cells with an osteogenic potential. The objective of this study was to evaluate the cell proliferation, viability and osteocyte differentiation of equine-derived bone marrow mesenchymal stem cells (EqMSCs) when seeded onto biocompatible and biodegradable calcium-deficient hydroxyapatite (CdHA) tubular-shaped bacterial cellulose scaffolds (BC-TS) of various sizes. The biocompatible gel-like BC-TS was synthesized using the bacterium Gluconacetobacter sucrofermentans under static culture in oxygen-permeable silicone tubes. The BC-TS scaffolds were modified using a periodate oxidation to yield biodegradable scaffolds. Additionally, CdHA was deposited in the scaffolds to mimic native bone tissues. The morphological properties of the resulting BC-TS and its composites were characterized using scanning electron microscopy. The ability of the BC-TS and its composites to support and maintain EqMSCs growth, proliferation and osteogenic differentiation in vitro was also assessed. BC-TS and its composites exhibited aligned nanofibril structures. MTS assay demonstrated increasing proliferation and viability with time (days 1, 2 and 3). Cell-scaffold constructs were cultured for 8 days under osteogenic conditions and the resulting osteocytes were positive for alizarin red. In summary, biocompatible and biodegradable CdHA BC-TS composites support the proliferation, viability and osteogenic differentiation of EqMSCs cultured onto its surface in vitro, allowing for future potential use for tissue engineering therapies.

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Cell proliferation, viability, and in vitro differentiation of equine mesenchymal stem cells seeded on bacterial cellulose hydrogel scaffolds

Cited by 101 publications

References 51 publications

Novel Bionanocellulose/κ-Carrageenan Composites for Tissue Engineering

Novel Bionanocellulose/κ-Carrageenan Composites for Tissue Engineering

Bacterial Cellulose

Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Biodegradable Calcium-deficient Hydroxyapatite Tubular Bacterial Cellulose Composites

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