Cell proliferation of silk proteins obtained from Bombyx mori silkworm varieties

Chung, Daeun; Kim, Sung-Kook; Jo, You‐Young; Kweon, HaeYong; Lee, Kwang-Gill; Kim, Hyun-Bok

doi:10.7852/jses.2015.53.2.92

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…Particularly, Heuros of Australia is focusing on synthesizing recombinant proteins to develop culture media that do not contain antibiotics, hormones, and serum [ 21 ]. However, in the case of Korea, both FBS-based and serum-free media rely mostly on imports; therefore, the development of its own product is a priority for Korea [ 22 ]. For the industrialization of cultivated meat, persistent mass production is essential; therefore, cultivating meat in a three-dimensional (3D) space instead of a two-dimensional (2D) space can help further increase production because cells grow better when attached to a surface [ 23 ].…”

Section: Summary Of the Development Of Cultivated Meat Technologiesmentioning

confidence: 99%

Current technology and industrialization status of cell-cultivated meat

Lee,

Yun

et al. 2024

J Anim Sci Technol

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Interest and investment in cultivated meat are increasing because of the realization that it can effectively supply sufficient food resources and reduce the use of livestock. Nevertheless, accurate information on the specific technologies used for cultivated meat production and the characteristics of cultivated meat is lacking. Authorization for the use of cultivated meat is already underway in the United States, Singapore, and Israel, and other major countries are also expected to approve cultivated meat as food once the details of the intricate process of producing cultivated meat, which encompasses stages such as cell proliferation, differentiation, maturation, and assembly, is thoroughly established. The development and standardization of mass production processes and safety evaluations must precede the industrialization and use of cultivated meat as food. However, the technology for the industrialization of cultivated meat is still in its nascent stage, and the mass production process has not yet been established. The mass production process of cultivated meat may not be easy to disclose because it is related to the interests of several companies or research teams. However, the overall research flow shows that equipment development for mass production and cell acquisition, proliferation, and differentiation, as well as for three-dimensional production supports and bioreactors have not yet been completed. Therefore, additional research on the mass production process and safety of cultivated meat is essential. The consumer’s trust in the cultivated meat products and production technologies recently disclosed by some companies should also be analyzed and considered for guiding future developments in this industry. Furthermore, close monitoring by academia and the government will be necessary to identify fraud in the cultivated meat industry.

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Section: Summary Of the Development Of Cultivated Meat Technologiesmentioning

confidence: 99%

Current technology and industrialization status of cell-cultivated meat

Lee,

Yun

et al. 2024

J Anim Sci Technol

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“…Also, silk fibroin was recognized by the US Food and Drug Administration (FDA) as a biomaterial in 1993 [32]. Previous studies have shown that silk protein (sericin and fibroin) treatment is effective in cell proliferation and that there is a significant difference between silkworm varieties [33]. Sericin and fibroin from silkworm cocoons of five varieties were made to observe FBS substitution ability of silk proteins.…”

Section: Effect Of Silk Sericin and Fibroinmentioning

confidence: 99%

Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture

Jo¹,

Kweon²,

Ji³

et al. 2019

Microbiology and Biotechnology Letters

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Fetal Bovine Serum (FBS) is an essential substance added to animal cell culture medium. However, its composition is unclear causing problems such as development of an immune response when cultured cells are transplanted into the human body. In this study, silk sericin, silk fibroin, and hemolymph obtained from silkworms were added to the cell culture medium in order to determine if it can replace FBS. After establishment of the cell culture, cell proliferation and expression levels of cell growth-related genes were compared with those of control cells (cells cultured in the medium with 10% FBS). Results showed that the test group treated with silk fibroin extracted from a Korean silkworm variety, Kumokjam could replace 10% FBS. In addition, expression levels of cell growth related genes such as Fibronectin and TGF-β1 increased significantly in cells cultured using silk fibroin, depending on the concentration used in cell adhesion and cell proliferation [24]. To date, no studies have been conducted to find a replacement for FBS. Thus, this study was carried out to develop a substitute for FBS by using silkworm-derived alternatives such as silkworm hemolymph, silk sericin, and silk fibroin, which are cheap and have various physiological effects, cell promoting effects, and can be mass produced.

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“…The annual plant P. tinctorium has long been employed in the medical and dye industries (Cooksey, 2007;Hirota et al, 2014). It is also often used in many nations, particularly Korea and Japan, for traditional fabric staining as a source of blue color (Chung et al, 2005;Hirota et al, 2016). A substance called indigo, which was discovered from P. tinctorium, is widely used in traditional Chinese medicine and as a natural color in denim manufacturing (Kukuła-Koch et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium

Choi,

Kim,

Seo

et al. 2024

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Polygonum tinctorium has long been employed in the medical and dye industries in many nations, particularly in Korea and Japan, for traditional fabric staining as a source of blue color. The plant tissue culture method has proven to be a rapid and sustainable approach for the regeneration of various plant species, particularly those with medicinal or ornamental value. In this study, we explored the in vitro root regeneration and growth of P. tinctorium in response to different growth media and gelling agents. Among the tested media, Schenk and Hildebrandt medium (SH medium) demonstrated superior performance, resulting in the highest number and length of roots per explant. SH media produced about 53 and 18% higher root per explant and a 40% and 14% increase in root length compared to B5 and MS media, respectively. Half strength of SH medium proved to be the optimal condition for both root number (7.46 roots per explant) and root length (3.81 mm). Among the gelling agents, 2 g/L of Gelrite medium was most effective in promoting the highest and tallest roots. These findings have the potential to enhance rooting abilities in various crops, particularly in the case of medicinal and ornamental plants, and may offer valuable insights for future industrial-scale root production of P. tinctorium.

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Cell proliferation of silk proteins obtained from Bombyx mori silkworm varieties

Cited by 4 publications

References 5 publications

Current technology and industrialization status of cell-cultivated meat

Current technology and industrialization status of cell-cultivated meat

Silk Protein as a Fetal Bovine Serum Substitute for Animal Cell Culture

Influence of medium and gelling agents concentration on in vitro rooting of Polygonum tinctorium

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