Cell proliferation is induced in renal cell carcinoma through miR‑92a‑3p upregulation by targeting FBXW7

Zeng, Rong; Huang, Jing; Sun, Yujie; Luo, Juan

doi:10.3892/ol.2020.11443

Cited by 15 publications

(12 citation statements)

References 44 publications

(45 reference statements)

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“…The roles of miR-92a-3p in cancers have been extensively investigated. In renal cell carcinoma, miR-92a-3p expression level is signi cantly upregulated and promotes cell proliferation by targeting FBXW7 (32). In gastric cancer (GC), the expression of miR-92a-3p is associated with tumor size, lymph node in ltration and distant metastasis, acting as an independent prognostic factor of poor survival in patients with GC.…”

Section: Discussionmentioning

confidence: 99%

25-hydroxycholesterol Regulates Migration, Invasion and EMT of Colorectal Cancer through miR-92a-3p/ACAA1/NF-κB Pathway

R²,

et al. 2021

Preprint

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Background: Colorectal cancer (CRC) is one of the most common malignances worldwide. Several studies suggest a positive association between high plasma cholesterol level and CRC. 25-hydroxycholesterol (25-HC) is enzymatically produced by cholesterol 25-hydorxylase in various organs and is involved in many processes. However, the critical role of 25-HC in the tumor growth and progression of CRC is largely unknown. Methods: CCK-8 assay, flow cytometry and Transwell migration and invasion assays were used to determine the effects of 25-HC on CRC cells proliferation, apoptosis and metastasis. Subcutaneous xenograft model and intra-splenic injection mouse model were established to investigate the effects of 25-HC on CRC in vivo. Immunohistochemistry staining was performed to determine the matrix metalloproteinases (MMPs) expressions in mice tumors and acetyl-CoA acyltransferase 1 (ACAA1) expression in human CRC tissues. The expressions of E-cadherin, N-cadherin and Vimentin were examined by immunofluorescent staining. MiR-92a-3p mimic, inhibitor and ACAA1 vector were constructed and transfected into LoVo cells. Results: 25-HC promotes CRC cells migration, invasion, and metastasis both in vitro and in vivo without affecting cells proliferation and apoptosis, accompanied by the upregulation of the expressions of MMPs and epithelial-mesenchymal transition (EMT) related markers. Mechanistically, miR-92a-3p expression is significantly elevated after 25-HC stimulation, while ACAA1 expression is down-regulated and negatively associated with tumor progression. Luciferase reporter assay confirms that miR-92a-3p could directly target ACAA1. Subsequent investigation indicates that nuclear factor (NF)-κB signaling is the downstream pathways of miR-92a-3p-ACAA1 axis in CRC cells. Conclusions: 25-HC promotes CRC cells metastasis by regulating cells migration, invasion and EMT through miR-92a-3p/ACAA1/NF-κB pathway.Trial registration: The current study was approved by the Ethics Committee of the First Affiliated Hospital, Zhejiang University on March22, 2018. The permission number was 2018-706 and 2020-1000.

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Section: Discussionmentioning

confidence: 99%

25-hydroxycholesterol Regulates Migration, Invasion and EMT of Colorectal Cancer through miR-92a-3p/ACAA1/NF-κB Pathway

R²,

et al. 2021

Preprint

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“…miR-92a-3p is known to be involved in apoptosis or necrosis, which is a member of the miR-17-92 cluster. In addition, miR‑92a‑3p has been shown to significantly increase cell growth and colony formation in renal cancer cells [ 77 ] ( Fig. 3 ).…”

Section: The Role Of Micrornas In Necroptotic Cell Death Pathwaysmentioning

confidence: 99%

The role of microRNAs in cell death pathways

Jang

Lee

2021

Yeungnam Univ J Med

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MicroRNAs (miRNAs) are a class of noncoding RNAs that negatively regulate target messenger RNAs. In multicellular eukaryotes, numerous miRNAs perform basic cellular functions, including cell proliferation, differentiation, and death. Abnormal expression of miRNAs weakens or modifies various apoptosis pathways, leading to the development of human cancer. Cell death occurs in an active manner that maintains tissue homeostasis and eliminates potentially harmful cells through regulated cell death processes, including apoptosis, autophagic cell death, and necroptosis. In this review, we discuss the involvement of miRNAs in regulating cell death pathways in cancers and the potential therapeutic functions of miRNAs in cancer treatment.

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“… 7 , 8 miR‐92a is a novel miRNA molecule discovered in recent years. It is considered to be a tumor promoter in colorectal cancer, 9 liver cancer, 10 gastric cancer, 11 and renal cell carcinoma 12 where it shows up‐regulated expression and appears to regulate cell functions such as tumor cell proliferation, migration, and apoptosis. However, the function of miR‐92a in cervical cancer and its mechanism of action are still unclear.…”

Section: Introductionmentioning

confidence: 99%

miR‐92a promotes cervical cancer cell proliferation, invasion, and migration by directly targeting PIK3R1

Wang¹,

Chen²,

Zheng³

et al. 2021

Clinical Laboratory Analysis

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Objective To clarify the role of miR‐92a in regulating the malignant progression of cervical cancer and its specific molecular mechanism. Methods qRT‐PCR was used to detect the differential expression of miR‐92a in cervical cancer and adjacent tissues. The effects of overexpression of miR‐92a on the proliferation, migration, and invasion of HeLa and SiHa cells were tested. Luciferase assays and rescue experiments were used to investigate the regulatory mechanism of miR‐92a on its downstream gene PIK3R1 and their interaction in the progression of cervical cancer. Results miR‐92a was significantly up‐regulated in cervical cancer tissues. Overexpression of miR‐92a significantly increased the ability of cervical cancer cells to proliferate, migrate, and invade. PIK3R1 was identified as a downstream gene of miR‐92a. In cervical cancer tissues, PIK3R1 was found to be down‐regulated and negatively correlated with the level of miR‐92a. Overexpression of PIK3R1 reversed the promotional effect of overexpressed miR‐92a on the proliferation, migration, and invasion of cervical cancer. Conclusion miR‐92a is up‐regulated in cervical cancer tissues. miR‐92a promotes the malignant development of cervical cancer by negatively regulating PIK3R1.

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Cell proliferation is induced in renal cell carcinoma through miR‑92a‑3p upregulation by targeting FBXW7

Cited by 15 publications

References 44 publications

25-hydroxycholesterol Regulates Migration, Invasion and EMT of Colorectal Cancer through miR-92a-3p/ACAA1/NF-κB Pathway

25-hydroxycholesterol Regulates Migration, Invasion and EMT of Colorectal Cancer through miR-92a-3p/ACAA1/NF-κB Pathway

The role of microRNAs in cell death pathways

miR‐92a promotes cervical cancer cell proliferation, invasion, and migration by directly targeting PIK3R1

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