Cell proliferation in the developing lateral line system of zebrafish embryos

Laguerre, Laurent; Soubiran, Fabien; Ghysen, Alain; König, Norbert; Dambly‐Chaudière, Christine

doi:10.1002/dvdy.20343

Cited by 44 publications

(50 citation statements)

References 19 publications

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“…It has previously been noted that the progenitor cells of the primary primordium, primI, enter a phase of mitotic quiescence around 10 hpf and do not resume proliferation until 16 -18 hpf (Laguerre et al, 2005). These authors also noted that, whereas exposure to bromodeoxyuridine between 8 and 10 hpf labels most PLL neurons, exposure starting at 10 hpf labels only a few neurons, refining a previous finding that PLL neurons become postmitotic at some time between 8 and 13 hpf (Metcalfe, 1983).…”

Section: Discussionsupporting

confidence: 52%

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Sarrazin¹,

Nuñez²,

Sapède³

et al. 2010

Journal of Neuroscience

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The lateral line system of teleosts has recently become a model system to study patterning and morphogenesis. However, its embryonic origins are still not well understood. In zebrafish, the posterior lateral line (PLL) system is formed in two waves, one that generates the embryonic line of seven to eight neuromasts and 20 afferent neurons and a second one that generates three additional lines during larval development. The embryonic line originates from a postotic placode that produces both a migrating sensory primordium and afferent neurons. Nothing is known about the origin and innervation of the larval lines. Here we show that a "secondary" placode can be detected at 24 h postfertilization (hpf), shortly after the primary placode has given rise to the embryonic primordium and ganglion. The secondary placode generates two additional sensory primordia, primD and primII, as well as afferent neurons. The primary and secondary placodes require retinoic acid signaling at the same stage of late gastrulation, suggesting that they share a common origin. Neither primary nor secondary neurons show intrinsic specificity for neuromasts derived from their own placode, but the sequence of neuromast deposition ensures that neuromasts are primarily innervated by neurons derived from the cognate placode. The delayed formation of secondary afferent neurons accounts for the capability of the fish to form a new PLL ganglion after ablation of the embryonic ganglion at 24 hpf.

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Section: Discussionsupporting

confidence: 52%

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Sarrazin¹,

Nuñez²,

Sapède³

et al. 2010

Journal of Neuroscience

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“…Finally, we observed that the sox3-expressing p4 subdomain migrates posteriorly from its original domain along the body axis after the 20-somite stage (data not shown). Given that this migration trend of the p4 subdomain is similar to that of the posterior lateral line primordium, as revealed by morphological observation (Laguerre et al, 2005) and expression of the chemokine receptor cxcr4 (Chong et al, 2001;David et al, 2002), we suggest that the p4 subdomain represents the anlage of the posterior lateral line system.…”

Section: Early Development Of the Epibranchial Placode In Zebrafishsupporting

confidence: 56%

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Nikaido

Doi

Shimizu³

et al. 2006

Developmental Dynamics

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In vertebrates, cranial sensory ganglia are mainly derived from ectodermal placodes, which are focal thickenings at characteristic positions in the embryonic head. Here, we provide the first description of the early development of the epibranchial placode in zebrafish embryos using sox3 as a molecular marker. By the one-somite stage, we saw a pair of single sox3-expressing domains appear lateral to the future hindbrain. The sox3 domain, which is referred to here as the early lateral placode, is segregated during the early phase of segmentation to form a pax2a-positive medial area and a pax2a-negative lateral area. The medial area subsequently developed to form the otic placode, while the lateral area was further segregated along the anteroposterior axis, giving rise to four sox3-positive subdomains by 26 hr postfertilization. Given their spatial relationship with the expression of the markers for the epibranchial ganglion, as well as their positions and temporal changes, we propose that these four domains correspond to the facial, glossopharyngeal, vagal, and posterior lateral line placodes in an anterior-to-posterior order. The expression of sox3 in the early lateral placode was absent in mutants lacking functional fgf8, while implantation of fibroblast growth factor (FGF) beads restored the sox3 expression. Using SU5402, which inhibits the FGF signal, we were able to demonstrate that formation of both the early lateral domains and later epibranchial placodes depends on the FGF signal operating at the beginning of somitogenesis. Together, these data provide evidence for the essential role of FGF signals in the development of the epibranchial placodes. Developmental Dynamics 236:564 -571, 2007.

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“…We found that lef1 activity is required for the wave of cell proliferation that takes place in the PLL placode before the onset of migration, after a long period of quiescence extending from around 10 hpf to around 18 hpf (Laguerre et al, 2005). This early burst of DNA replication is Error bars indicate standard deviations, n ¼ 31 for the control, 27 for the morphants (5 lef1-MO injected embryos showed no phenotype at 3 days postfertilization [dpf] and were not included in the calculations).…”

Section: Discussionmentioning

confidence: 86%

“…Contrary to this early burst, DNA replication is asymmetrically distributed in the migrating primordium, with leading cells showing BrdU incorporation at a constant rate, whereas incorporation in the trailing cells only occurs in phasic pulses synchronized with events of neuromast deposition (Laguerre et al, 2005). It seems conceivable that, much like ubiquitous lef1 activity is required for ubiquitous proliferation in the presumptive tissue, Wnt signaling acting through lef1 is responsible for the continuous DNA replicating activity in the leading cells of the migrating primordium.…”

Section: Discussionmentioning

confidence: 99%

“…Because the early, ubiquitous expression of lef1 at 18-20 hpf nearly coincides with a wave of DNA replication throughout the presumptive primordium (Laguerre et al, 2005), we examined the effect of lef1 inactivation on BrdU incorporation at this stage. We observed a marked difference between control and morphant embryos (Fig.…”

Section: Lef1 Is Required For Cell Proliferation In the Pll Placodementioning

confidence: 99%

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lef1 controls patterning and proliferation in the posterior lateral line system of zebrafish

Gamba¹,

Cubedo²,

Lutfalla³

et al. 2010

Developmental Dynamics

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The embryonic development of the posterior lateral line of zebrafish involves the migration from head to tail of a primordium comprising approximately 100 cells, and the deposition at regular intervals of presumptive mechanosensory organs (neuromasts). Migration depends on the presence of chemokine SDF1 along the pathway, and on the asymmetrical distribution of chemokine receptors CXCR4 and CXCR7 in the primordium. Primordium polarization depends on Wnt signaling in the leading region. Here, we examine the role of a major effector of Wnt signaling, lef1, in this system. We show that, although its inactivation has no overt effect on the expression of cxcr4b and cxcr7b, lef1 contributes to their control. We also show that cell proliferation, which ensures constant primordium size despite successive rounds of cell deposition, is reduced upon lef1 inactivation. Because of this defect, the primordium runs short of cells and vanishes before the line has been completed. We conclude that lef1-mediated Wnt signaling is involved in various aspects of primordium migration, although part of this implication is masked by a high level of developmental redundancy. Developmental Dynamics 239:3163-3171,

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Cell proliferation in the developing lateral line system of zebrafish embryos

Cited by 44 publications

References 19 publications

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

lef1 controls patterning and proliferation in the posterior lateral line system of zebrafish

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