Cell proliferation in skeletal muscle following denervation or tenotomy

Abstract

Autoradiographic experiments using 3H-thymidine were designed to analyse cell proliferation which occurs in skeletal muscle after denervation and after tenotomy. In mouse tibialis anterior and tongue muscles during the first 24 h after denervation or tenotomy labelling levels were low and did not differ significantly from sham operated control muscles. By 48 h after denervation and tenotomy of tibialis anterior muscles, increased levels of labelling occurred in both muscle and connective tissue nuclei. Daily pulse labelling for 7 days after denervation produced a labelling level which was 8 times that of sham operated controls, 25--30% of the total nuclear population being labelled. Denervated muscles had twice the level of labelling compared to tenotomised muscles. These results provide conclusive evidence that both denervation and tenotomy stimulate cell proliferation in skeletal muscle and it is suggested that the increased numbers of labelled muscle nuclei are likely to be the result of mitotic activity in muscle satellite cells.

Aging of Skeletal Muscle Does Not Affect the Response of Satellite Cells to Denervation

“…However, these studies have generated some controversy concerning the timing of increases of the number of SCs in denervated muscles. Despite the fact that several authors reported a marked increase in the number of SCs during the first week following denervation (Hanzlikova et al 1975;McGeachie and Allbrook 1978), a few other studies demonstrated such increases between 2 and 4 weeks after nerve transection (Ontell 1974;Snow 1983). These findings are in contrast to those obtained in our laboratory.…”

Expression pattern of M‐cadherin in normal, denervated, and regenerating mouse muscles

“…For denervated muscles this appeared surprising since enhanced satellite cell proliferation, as compared with normal muscle, had been previously documented (Schultz et al, 1978;McGeachie, 1985;Murray and Robbins, 1982a). Moderate labelling indices (e.g., cummulative index of 11% over a period of 7 days after denervation, McGeachie and Allbrook, 1978) and large interanimal variability (Murray and Robbins, 1982a) may account for the failure to detect labelled satellite cells in the present study. However, in the same muscle sections BrDU-labelled cells in interstitial spaces (Fig.…”

Propriétés des fibres musculaires squelettiques. 1. Influence de l'innervation motrice

“…La dénervation stimule la prolifération cellulaire, en particulier celle des cellules satellites (McGeachie et Allbrook, 1978 ;Murray et Robbins, 1982 ;Snovv, 1983 ;McGeachie, 1985). Des phénomènes de dégénérescence affectant à la fois le métabolisme, la structure des myofilaments et des terminaisons nerveuses apparaissent.…”