Cell proliferation and migration in silk fibroin 3D scaffolds

Mandal, Biman B.; Kundu, Subhas C.

doi:10.1016/j.biomaterials.2009.02.006

Cited by 497 publications

(356 citation statements)

References 30 publications

Supporting

Mentioning

340

Contrasting

Unclassified

Order By: Relevance

“…Lyophilization has been used to fabricate porous scaffolds for tissue engineering 32) . Organic solvents such as acetic acid and cytotoxic chemicals such as glutaraldehyde 20) for chemical crosslink reaction were mainly used.…”

Section: Discussionmentioning

confidence: 99%

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

et al. 2017

View full text Add to dashboard Cite

This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (−30, −80 and −196 o C), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro. Freezing temperature did not affect porosity while high freezing temperature (−30 o C) increased pore size. The high gelatin concentration group (0.6%AC+2.4%G) had decreased porosity and pore size. Freezing time and 500 μM fluvastatin did not affect pore structures. The cytotoxicity and cell proliferation assays revealed that ACG sponges had no cytotoxic effects on human mesenchymal stromal cell growth and proliferation. These results indicate that ACG sponge may be a good biomaterial scaffold for bone regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Characteristics of scaffolds (1) Biocompatibility, to avoid unwanted host tissue responses to the implant (Ma, & Langer, 1999). (2) It should have the excellent surface chemistry to allow attachment, migration, proliferation, and differentiation of the cells (Mandal and Kundu, 2008b;2009a;2009b). (3) Interconnected pores with proper pore size to support cell infiltration and vascularization (Hutmacher 2001;Karageorgiou & Kaplan, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biomaterial Scaffold Fabrication Techniques for Potential Tissue Engineering Applications

Subia¹,

Kundu²,

Kundu³

2010

Tissue Engineering

116

View full text Add to dashboard Cite

“…A number of studies have been done to reveal the effects of 2D versus 3D cell culture on differentiation, [21][22][23][24][25] drug metabolism, [26][27][28][29][30] gene expression and protein synthesis, [31][32][33][34][35] general cell function, [36][37][38][39][40] increase of in vivo relevance, 31,[41][42][43] morphology, [44][45][46][47] proliferation, 26,[48][49][50][51] response to stimuli, 41,[52][53][54] viability, 36,[55][56][57] and migration. [58][59][60][61] Adding the third dimension to cellular environment provides them with more in vivo-like morphology, behaviors, and intercellular interactions for understanding cytology in more physiological conditions. 3D scaffolds are introduced to mimic cells' in vivo environment while cultured in vitro.…”

Section: Introductionmentioning

confidence: 99%

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

et al. 2012

View full text Add to dashboard Cite

In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells' migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate

show abstract

Cell proliferation and migration in silk fibroin 3D scaffolds

Cited by 497 publications

References 30 publications

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

Biomaterial Scaffold Fabrication Techniques for Potential Tissue Engineering Applications

Electrotaxis of lung cancer cells in ordered three-dimensional scaffolds

Contact Info

Product

Resources

About