Cell proliferation and hair cell addition in the ear of the goldfish, Carassius auratus

Lanford, Pamela J.; Presson, Joelle C.; Popper, Arthur N.

doi:10.1016/0378-5955(96)00110-4

Cited by 36 publications

(14 citation statements)

References 35 publications

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“…In mammals, age-related hearing loss usually occurs first at the high frequencies, while we see the greatest elevation in thresholds in the center of the zebrafish audiogram (400–1600 Hz). While a recent study suggested that zebrafish show signs of age-related hearing loss (Wang et al, 2015), fish continually produce hair cells throughout life, and the extent of possible age effects are not understood (Lombarte and Popper, 1994; Lanford et al, 1996). We therefore consider it unlikely that the auditory variability in our study results from age-related hearing loss.…”

Section: Discussionmentioning

confidence: 99%

Hearing sensitivity differs between zebrafish lines used in auditory research

et al. 2016

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Zebrafish are increasingly used in auditory studies, in part due to the development of several transgenic lines that express hair cell-specific fluorescent proteins. However, it is largely unknown how transgene expression influences auditory phenotype. We previously observed reduced auditory sensitivity in adult Brn3c:mGFP transgenic zebrafish, which express membrane-bound green fluorescent protein (GFP) in sensory hair cells. Here, we examine the auditory sensitivity of zebrafish from multiple transgenic and background strains. We recorded auditory evoked potentials in adult animals and observed significantly higher auditory thresholds in three lines that express hair cell-specific GFP. There was no obvious correlation between hair cell density and auditory thresholds, suggesting that reduced sensitivity was not due to a reduction in hair cell density. FM1-43 uptake was reduced in Brn3c:mGFP fish but not in other lines, suggesting that a mechanotransduction defect may be responsible for the auditory phenotype in Brn3c animals, but that alternate mechanisms underlie the increased AEP thresholds in other lines. We found reduced prepulse inhibition (a measure of auditory-evoked behavior) in larval Brn3c animals, suggesting that auditory defects develop early in this line. We also found significant differences in auditory sensitivity between adults of different background strains, akin to strain differences observed in mouse models of auditory function. Our results suggest that researchers should exercise caution when selecting an appropriate zebrafish transgenic or background strain for auditory studies.

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Section: Discussionmentioning

confidence: 99%

Hearing sensitivity differs between zebrafish lines used in auditory research

et al. 2016

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“…In our study, a low level of BrdU labeling was seen in the saccules of untreated control zebrafish, consistent with a previous study in which non-acoustically exposed adult zebrafish exhibited hair cells labeled for BrdU, five days after BrdU injection (Higgs et al, 2002). BrdU-labeled hair cells have also been identified in thin sections of control goldfish saccules (Lanford et al, 1996). In contrast, auditory hair cells and supporting cells in the post-embryonic chick basilar papilla/cochlea are quiescent in the absence of a damaging stimulus (Corwin and Cotanche, 1988; Katayama and Corwin, 1989).…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation follows acoustically-induced hair cell bundle loss in the zebrafish saccule

Schuck

Smith

2009

Hearing Research

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Fishes are capable of regenerating sensory hair cells in the inner ear after acoustic trauma. However, a time course of auditory hair cell regeneration has not been established for zebrafish. Adult zebrafish (Danio rerio) were exposed to a 100 Hz pure tone at 179 dB re 1 μPa RMS for 36 hours and then allowed to recover for 0 to 14 days before morphological analysis. Hair cell bundle loss and recovery were determined using phalloidin to visualize hair bundles. Cell proliferation was quantified through bromodeoxyuridine (BrdU) labeling. Immediately following sound exposure, zebrafish saccules exhibited significant hair bundle damage (eg., splayed, broken, and missing stereocilia) and loss (ie., missing bundles and lesions in the epithelia) in the caudal region. Hair bundle counts increased over the course of the experiment, reaching pre-treatment levels at 14 days post-sound exposure (dpse). Low levels of proliferation were observed in untreated controls, indicating that some cells of the zebrafish saccule are mitotically active in the absence of a damaging event. In sound-exposed fish, cell proliferation peaked two dpse in the caudal region, and to a lesser extent in the rostral region. This proliferation was followed by an increase in numbers of cuticular plates with rudimentary stereocilia and immature-like hair bundles at 7 and 14 dpse, suggesting that at least some of the saccular cell proliferation resulted in newly formed hair cells. This study establishes a time course of hair cell bundle regeneration in the zebrafish inner ear and demonstrates that cell proliferation is associated with the regenerative process.

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“…Such is the case in fish, amphibians, reptiles, and birds, where mechanoreceptive hair cells are produced throughout life and can be regenerated from supporting cells to restore lost hearing, balance, and lateral line functions (Corwin, 1981, 1985, 1986; Corwin and Cotanche, 1988; Jorgensen and Mathiesen, 1988; Ryals and Rubel, 1988; Popper and Hoxter, 1990; Lanford et al, 1996; Warchol, 2011). In those species, hair cell death leads to robust proliferation of supporting cells, whose progeny give rise to replacement hair cells that become innervated and restore sensory functions within weeks.…”

Section: Introductionmentioning

confidence: 99%

In VivoProliferative Regeneration of Balance Hair Cells in Newborn Mice

Burns

Cox²,

Thiede³

et al. 2012

J. Neurosci.

100

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The regeneration of mechanoreceptive hair cells occurs throughout life in non-mammalian vertebrates and allows them to recover from hearing and balance deficits that affect humans and other mammals permanently. The irreversibility of comparable deficits in mammals remains unexplained, but often has been attributed to steep embryonic declines in cellular production. However, recent results suggest that gravity-sensing hair cells in murine utricles may increase in number during neonatal development, raising the possibility that young mice might retain sufficient cellular plasticity for mitotic hair cell regeneration. To test for this we used neomycin to kill hair cells in utricles cultured from mice of different ages and found that proliferation increased ten-fold in damaged utricles from the youngest neonates. To kill hair cells in vivo, we generated a novel mouse model that uses an inducible, hair-cell-specific CreER allele to drive expression of diptheria toxin fragment A (DTA). In newborns, induction of DTA expression killed hair cells and resulted in significant, mitotic hair cell replacement in vivo, which occurred days after the normal cessation of developmental mitoses that produce hair cells. DTA expression induced in five-day-old mice also caused hair cell loss, but no longer evoked mitotic hair cell replacement. These findings show that regeneration limits arise in vivo during the postnatal period when the mammalian balance epithelium’s supporting cells differentiate unique cytological characteristics and lose plasticity, and they support the notion that the differentiation of those cells may directly inhibit regeneration or eliminate an essential, but as yet unidentified pool of stem cells.

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Cell proliferation and hair cell addition in the ear of the goldfish, Carassius auratus

Cited by 36 publications

References 35 publications

Hearing sensitivity differs between zebrafish lines used in auditory research

Hearing sensitivity differs between zebrafish lines used in auditory research

Cell proliferation follows acoustically-induced hair cell bundle loss in the zebrafish saccule

In VivoProliferative Regeneration of Balance Hair Cells in Newborn Mice

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