Cell proliferation and expression of connexins differ in melanotic and amelanotic canine oral melanomas

Teixeira, Tarso Felipe; Gentile, Luciana Boffoni; Silva, Tereza Cristina da; Mennecier, Gregory; Chaible, Lucas Martins; Cogliati, Bruno; Roman, Marco Antonio Leon; Gioso, Marco Antônio; Dagli, M.L.

doi:10.1007/s11259-013-9580-z

Cited by 20 publications

(29 citation statements)

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“…HMB45, molecular weight of 7000 kDa, is a specific protein marker for melanoma cells, and mainly distributed in the cytoplasm [ 17 ]. Further, the expression of HMB45 in the lung tissue of tumor-bearing C57BL/6 mice was assessed by the immunohistochemical staining method.…”

Section: Resultsmentioning

confidence: 99%

Antitumor and antimetastatic activities of chloroform extract of medicinal mushroom Cordyceps taii in mouse models

Liu

Zhang

Liang

et al. 2015

BMC Complement Altern Med

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BackgroundCordyceps taii, an entomogenous fungus native to south China, is a folk medicine with varieties of pharmacological activities including anticancer effect. To validate the ethnopharmacological claim against cancer, the antitumor and antimetastatic activities of chloroform extract of C. taii (CFCT) were investigated in vivo.MethodsThe in vitro cytotoxic activities of CFCT against human lung cancer (A549) and gastric cancer (SGC-7901) cells were evaluated using the Sulforhodamine B (SRB) assay. In vivo anti tumor and antimetastatic activities, Kunming mice bearing sarcoma 180 and C57BL/6 mice bearing melanoma B16F10 were employed, respectively. The antitumor effects of CFCT were completely evaluated on the basis of the tumor weight, survival time, histologic analysis, and immune organ indices. The histopathological change, metastatic foci and malignant melanoma specific marker HMB45 in the lung tissue were detected for the evaluation of the antimetastatic activity of CFCT.ResultsCFCT exhibited dose- and time-dependent cytotoxicities against A549 and SGC-7901 cells with the IC50 values of 30.2 and 65.7 μg/mL, respectively. Furthermore, CFCT at a dose of 50 or 100 mg/kg could significantly inhibit the tumor growth in vivo and prolonged the survival time in two different models as compared with the model group, especially when combined with the CTX at a low dose rate. And it also increased spleen index of Kunming mice and thymus index of C57BL/6 mice. Meanwhile, histologic analysis illustrated that CFCT alone or in combination with CTX could induce tumor tissue necrosis of both models. In addition, CFCT at a dose of 50 or 100 mg/kg inhibited the lung metastasis of melanoma B16F10 in tumor-bearing C57BL/6 mice. The antimetastatic effect was also observed when CFCT was used in combination with CTX. In comparison to any other groups, CFCT at a dose of 100 mg/kg could effectively enhance the GSH-Px activities of various tissues in tumor-bearing C57BL/6 mice.ConclusionsThese findings demonstrate that CFCT has potent in vivo antitumor and antimetastatic activities, and may be helpful to the development of anticancer chemopreventive agents from C. taii.

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Section: Resultsmentioning

confidence: 99%

Antitumor and antimetastatic activities of chloroform extract of medicinal mushroom Cordyceps taii in mouse models

Liu

Zhang

Liang

et al. 2015

BMC Complement Altern Med

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“…Histopathologically, melanoma is categorized into melanotic and amelanotic types according to melanin pigmentation (11). MM tumors are composed of a variety of cell types: Epithelioid, spindled, clear-cell, plasmacytoid and mixed-cell (12).…”

Section: Discussionmentioning

confidence: 99%

Undifferentiated sinonasal malignant melanoma: A case report

Huang

et al. 2018

Oncol Lett

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Undifferentiated sinonasal malignant melanoma (MM) is a rare type of tumor, which can be easily misdiagnosed. The present study reports a 41-year-old male patient who presented with a 4-day history of epistaxis. Clinical examination and radiological imaging lead to the detection of a mass in the right sinonasal region. Histopathological examination revealed that the mass was composed of malignant epithelioid cells arranged in nests and sheets. These cells displayed a hemangiopericytoma-like pattern with antler-like branching vessels. Immunohistochemical staining revealed that the tumor cells exhibited negative expression of melanocytic markers. This increased the difficulty of distinguishing undifferentiated MM from other malignant tumors located in the sinonasal area, particularly undifferentiated nasopharyngeal carcinoma. The diagnosis of undifferentiated MM was determined by ultrastructures, including the mature melanosomes and premelanosomes, in tumor cells by transmission electron microscopy. The present study suggests that the analysis of cancer stem cell marker and vasculogenic mimicry may be an important auxiliary tool for the diagnosis of MM.

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“…Conforme Teixeira et al (2014), observou-se que animais portadores de melanomas amelanocíticos apresentaram uma sobrevida mais curta e proliferação celular mais elevada em comparação aos animais com melanomas melanóticos. No melanoma maligno a lesão não é encapsulada, tendo como consequência um crescimento muito agressivo e uma rápida infiltração local dos tecidos adjacentes, incluindo as amígdalas e o osso (em mais de 50% dos casos), causando a sua lise e a esfoliação dentária (Ettinger and Feldman, 2004).…”

Section: Discussionunclassified

Melanoma amelanótico oral en perro joven: Reporte de un caso

Moreira

Rodrigues

Silva

et al. 2017

Pubvet

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RESUMO.A cavidade oral é bastante acometida por neoplasias e corresponde ao quarto local mais frequente em pequenos animais. O melanoma é uma neoplasia maligna caracterizada com muita ou pouca produção de melanina, tendo o último um comportamento mais agressivo. O presente trabalho objetiva relatar o caso de um cão, macho, da raça Dachshund, de dois anos de idade que apresentava leve aumento da região infraorbitária e na cavidade oral uma massa de coloração avermelhada, com áreas enegrecidas, de consistência firme, localizada na região maxilar direita. O exame histopatológico sugeriu melanoma amelanótico. O paciente foi submetido a tratamento quimioterápico com carboplatina (300 mg/m², EV) a cada 3 semanas totalizando 3 sessões e corticoterapia com prednisolona (2,2 mg/kg, SID, VO) uso contínuo, seguido de 3 sessões de criocirurgia com intervalo de 15 dias. Devido à agressividade da neoplasia e o péssimo estado geral do animal, foi realizada a eutanásia seguida à necropsia, que constatou metástase em linfonodo submandibular direito. O melanoma amelanótico é frequentemente diagnosticado na clínica de pequenos animais, necessitando de mais estudos para desenvolvimento de tratamentos eficazes para este tipo de neoplasia. Palavras chave: Cavidade oral, neoplasia, melanoma, cãoOral amelanotic melanoma in young dog: Case report ABSTRACT. The oral cavity is severely affected by neoplasias and corresponds to the fourth most frequent site in small animals. Melanoma is a malignant neoplasm characterized with much or little production of melanin, the latter having a more aggressive behavior. The present work aims to report the case of a 2 year old dachshund male dog with a slight increase in the infraorbital region and in the oral cavity a mass of reddish color with blackish areas of firm consistency located in the region Right jaw Histopathologic examination suggested amelanotic melanoma. The patient underwent chemotherapy with carboplatin (300 mg / m², EV) every 3 weeks for 3 sessions and corticosteroid therapy with prednisolone (2.2 mg / kg, SID, VO) continuous use, followed by 3 sessions of cryosurgery with Interval of 15 days. Due to the aggressiveness of the neoplasia and the poor general state of the animal, euthanasia followed by necropsy, which revealed metastasis in the right submandibular lymph node. Amelanotic melanoma is frequently diagnosed in the small

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Cell proliferation and expression of connexins differ in melanotic and amelanotic canine oral melanomas

Cited by 20 publications

References 33 publications

Antitumor and antimetastatic activities of chloroform extract of medicinal mushroom Cordyceps taii in mouse models

Antitumor and antimetastatic activities of chloroform extract of medicinal mushroom Cordyceps taii in mouse models

Undifferentiated sinonasal malignant melanoma: A case report

Melanoma amelanótico oral en perro joven: Reporte de un caso

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