Cell Polarity in <i>Saccharomyces cerevisiae</i> Depends on Proper Localization of the Bud9 Landmark Protein by the EKC/KEOPS Complex

Kato, Yu; Kawasaki, Hiroshi; Ohyama, Yoshifumi; Morishita, Takashi; Iwasaki, Hiroshi; Kokubo, Tetsuro; Hirano, Hisashi

doi:10.1534/genetics.111.128231

Cited by 7 publications

(7 citation statements)

References 38 publications

(71 reference statements)

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“…Pcc1, Gon7, Kae1 and Bud32, for example, are recruited to several genomic loci and affect gene transcription [65]. Kae1 contributes to faithful chromosome segregation [64] while Bud32, Cgi121, Gon7 regulate cell polarity in bud-site selection [78]. Additionally, Bud32, Kae1 and Pcc1 are essential for a universal tRNA modification called threonyl carbamoyl adenosine (t6A), for which Cgi121 is dispensable [66].…”

Section: Discussionmentioning

confidence: 99%

Telomerase-Null Survivor Screening Identifies Novel Telomere Recombination Regulators

Tang

Liu

et al. 2013

PLoS Genet

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Telomeres are protein–DNA structures found at the ends of linear chromosomes and are crucial for genome integrity. Telomeric DNA length is primarily maintained by the enzyme telomerase. Cells lacking telomerase will undergo senescence when telomeres become critically short. In Saccharomyces cerevisiae, a very small percentage of cells lacking telomerase can remain viable by lengthening telomeres via two distinct homologous recombination pathways. These “survivor” cells are classified as either Type I or Type II, with each class of survivor possessing distinct telomeric DNA structures and genetic requirements. To elucidate the regulatory pathways contributing to survivor generation, we knocked out the telomerase RNA gene TLC1 in 280 telomere-length-maintenance (TLM) gene mutants and examined telomere structures in post-senescent survivors. We uncovered new functional roles for 10 genes that affect the emerging ratio of Type I versus Type II survivors and 22 genes that are required for Type II survivor generation. We further verified that Pif1 helicase was required for Type I recombination and that the INO80 chromatin remodeling complex greatly affected the emerging frequency of Type I survivors. Finally, we found the Rad6-mediated ubiquitination pathway and the KEOPS complex were required for Type II recombination. Our data provide an independent line of evidence supporting the idea that these genes play important roles in telomere dynamics.

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Section: Discussionmentioning

confidence: 99%

Telomerase-Null Survivor Screening Identifies Novel Telomere Recombination Regulators

Tang

Liu

et al. 2013

PLoS Genet

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“…Another hint in this puzzle is the fact that mutants of EKC/KEOPS complex components in yeast have random budding patterns 15 , 22 . This could also contribute with the EKC/KEOPS mutant slow-growth phenotype.…”

Section: Bud32/prpk Is Part Of the Ancient Ekc/keops Complexmentioning

confidence: 99%

“…KEOPS/EKC complex has been implicated in telomeres maintenance, 13 transcriptional regulation, 14 budding site selection 15 and chemical modification of tRNAs (tRNAs). 16 , 17 The core constituents of the KEOPS complex are present in Drosophila , but their physical interactions have not been reported yet.…”

Section: Bud32/prpk Is Part Of the Ancient Ekc/keops Complexmentioning

confidence: 99%

TheDrosophilaEKC/KEOPS complex

Rojas-Benítez

Ibar²,

Glavic³

2013

Fly

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The TOR signaling pathway is crucial in the translation of nutritional inputs into the protein synthesis machinery regulation, allowing animal growth. We recently identified the Bud32 (yeast)/PRPK (human) ortholog in Drosophila, Prpk (p53-related protein kinase), and found that it is required for TOR kinase activity. Bud32/PRPK is an ancient and atypical kinase conserved in evolution from Archeae to humans, being essential for Archeae. It has been linked with p53 stabilization in human cell culture and its absence in yeast causes a slow-growth phenotype. This protein has been associated to KEOPS (kinase, putative endopeptidase and other proteins of small size) complex together with Kae1p (ATPase), Cgi-121 and Pcc1p. This complex has been implicated in telomere maintenance, transcriptional regulation, bud site selection and chemical modification of tRNAs (tRNAs). Bud32p and Kae1p have been related with N6-threonylcarbamoyladenosine (t6A) synthesis, a particular chemical modification that occurs at position 37 of tRNAs that pair A-starting codons, required for proper translation in most species. Lack of this modification causes mistranslations and open reading frame shifts in yeast. The core constituents of the KEOPS complex are present in Drosophila, but their physical interaction has not been reported yet. Here, we present a review of the findings regarding the function of this complex in different organisms and new evidence that extends our recent observations of Prpk function in animal growth showing that depletion of Kae1 or Prpk, in accordance with their role in translation in yeast, is able to induce the unfolded protein response (UPR) in Drosophila. We suggest that EKC/KEOPS complex could be integrating t6A-modified tRNA availability with translational rates, which are ultimately reflected in animal growth.

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“…Bud3 and Bud4 are important for establishing axial budding pattern by recruiting Axl1 and Axl2. The third group contains Bud7, Bud8, Bud9, Rax1, and Rax2 that are specific for the bipolar pattern (Zahner et al 1996;Kato et al 2011). Bud8 marks the distal pole while Bud9 marks the proximal pole (Zahner et al 1996;Schenkman et al 2002).…”

Section: Division-site Selection and Actomyosin Contractile-ring Assementioning

confidence: 99%

Molecular mechanisms of contractile-ring constriction and membrane trafficking in cytokinesis

Gerien

2018

Biophys Rev

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In this review, we discuss the molecular mechanisms of cytokinesis from plants to humans, with a focus on contribution of membrane trafficking to cytokinesis. Selection of the division site in fungi, metazoans, and plants is reviewed, as well as the assembly and constriction of a contractile ring in fungi and metazoans. We also provide an introduction to exocytosis and endocytosis, and discuss how they contribute to successful cytokinesis in eukaryotic cells. The conservation in the coordination of membrane deposition and cytoskeleton during cytokinesis in fungi, metazoans, and plants is highlighted.

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Cell Polarity in Saccharomyces cerevisiae Depends on Proper Localization of the Bud9 Landmark Protein by the EKC/KEOPS Complex

Cited by 7 publications

References 38 publications

Telomerase-Null Survivor Screening Identifies Novel Telomere Recombination Regulators

Telomerase-Null Survivor Screening Identifies Novel Telomere Recombination Regulators

TheDrosophilaEKC/KEOPS complex

Molecular mechanisms of contractile-ring constriction and membrane trafficking in cytokinesis

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