Cell‐Penetrating Peptides for Nucleic Acid Delivery – An Update

Vivès, Éric; Josse, Emilie; Konate, Karidia; Deshayes, Sébastien; Boisguérin, Prisca

doi:10.1002/9783527835997.ch14

Cited by 1 publication

(1 citation statement)

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“…Interestingly, intra-hemocoelic injection of siRNAs complexed with EB1 induces a silencing response (Figure 12). This is in line with the well-known capacity of some CPPs to perform intracellular delivery of nucleic acids [67].…”

Section: Discussionsupporting

confidence: 84%

The Study of Cell-Penetrating Peptides to Deliver dsRNA and siRNA by Feeding in the Desert Locust, Schistocerca gregaria

Vogel

Santos

Huygens

et al. 2023

Insects

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RNA(i) interference is a gene silencing mechanism triggered by double-stranded (ds)RNA, which promises to contribute to species-specific insect pest control strategies. The first step toward the application of RNAi as an insecticide is to enable efficient gene silencing upon dsRNA oral delivery. The desert locust, Schistocerca gregaria is a devastating agricultural pest. While this species is responsive to dsRNA delivered by intra-hemocoelic injection, it is refractory to orally delivered dsRNA. In this study, we evaluated the capacity of five cell-penetrating peptides (CPPs) to bind long dsRNA and protect it from the locust midgut environment. We then selected the CPP EB1 for further in vivo studies. EB1:dsRNA complexes failed to induce RNAi by feeding. Interestingly, we observed that intra-hemocoelic injection of small-interfering (si)RNAs does not result in a silencing response, but that this response can be obtained by injecting EB1:siRNA complexes. EB1 also protected siRNAs from midgut degradation activity. However, EB1:siRNA complexes failed as well in triggering RNAi when fed. Our findings highlight the complexity of the dsRNA/siRNA-triggered RNAi in this species and emphasize the multifactorial nature of the RNAi response in insects. Our study also stresses the importance of in vivo studies when it comes to dsRNA/siRNA delivery systems.

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Section: Discussionsupporting

confidence: 84%